Plasma metabolomic profiling in workers with noise-induced hearing loss: a pilot study

Miao, Long; Wang, Boshen; Zhang, Juan; Yin, Lihong; Pu, Yuepu

doi:10.1007/s11356-021-15468-z

Cited by 17 publications

(31 citation statements)

References 66 publications

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“…Blood plasma was collected in three studies to investigate cisplatin-induced changes at the systemic level [ 20 ], the metabolomic blood profile in a patient with hearing loss due to mitochondrial mutation [ 21 ], and changes in the metabolomic analysis of plasma following noise exposure in humans [ 22 ]. The volumes used for analysis were larger but required collection in a heparinized tube followed by centrifugation before freezing [ 20 , 21 , 22 ].…”

Section: Resultsmentioning

confidence: 99%

“…The analytical methods used to perform the metabolomic analyses were NMR spectrometry in one study [ 7 ], GCMS in four studies [ 16 , 23 , 24 , 25 ], and LCMS in eight studies [ 15 , 17 , 18 , 19 , 20 , 21 , 22 ]. Mass spectrometry (GCMS and LCMS) has a greater sensitivity than NMR spectrometry; however, this technique suffers from limited reproducibility [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…Ceramide accumulation was observed, which provided a better understanding of the pathophysiological mechanism involved [ 21 ]. Another study analyzed the metabolomic profile of plasma in noise-exposed patients [ 22 ]. The analysis was performed by LCMS in 62 patients and 62 controls.…”

Section: Resultsmentioning

confidence: 99%

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Metabolomic Studies in Inner Ear Pathologies

et al. 2022

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Sensorineural hearing loss is the most common sensory deficit. The etiologies of sensorineural hearing loss have been described and can be congenital or acquired. For congenital non-syndromic hearing loss, mutations that are related to sites of cochlear damage have been discovered (e.g., connexin proteins, mitochondrial genes, etc.). For cytomegalovirus infection or auditory neuropathies, mechanisms are also well known and well researched. Although the etiologies of sensorineural hearing loss may be evident for some patients, the damaged sites and pathological mechanisms remain unclear for patients with progressive post-lingual hearing loss. Metabolomics is an emerging technique in which all metabolites present in a sample at a given time are analyzed, reflecting a physiological state. The objective of this study was to review the literature on the use of metabolomics in hearing loss. The findings of this review suggest that metabolomic studies may help to develop objective tests for diagnosis and personalized treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Metabolomic Studies in Inner Ear Pathologies

et al. 2022

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“…Further analysis revealed that DEPs involving in the significant signaling pathways consisted of some proteins (ITGA1, KNG1, CFI, FGF1, AKT2, and ATG5) closely related to autophagy and inflammatory response. Our recent studies indicated that autophagy and inflammatory response may be a key pathological mechanism involving in NIHL [ 15 ]. Thus, these six DEPs were selected for validation ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…In this current study, we found that a set of DEPs related to autophagy and inflammation including three upregulated DEPs (ITGA1, KNG1 and CFI) and three downregulated DEPs (FGF1, AKT2, and ATG5) were involved in the PI3K/AKT pathway, ECM-receptor interaction, and focal adhesion pathway signaling pathways and may be important biomarkers of NIHL. Our previous studies reported that autophagy and inflammation may be noticeable pathological mechanisms involved in the risk of NIHL [ 15 , 32 ]. Western blotting suggested that the expression levels of FGF1, AKT2, and ATG5 were decreased, but the ITGA1, KNG1 and CFI expression levels were upregulated in noise-exposed mice, compared to control mice.…”

Section: Discussionmentioning

confidence: 99%

TMT-Based Quantitative Proteomics Reveals Cochlear Protein Profile Alterations in Mice with Noise-Induced Hearing Loss

Miao

Zhang

Yin

et al. 2021

IJERPH

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Noise-induced hearing loss (NIHL) is a global occupational disease affecting health. To date, genetic polymorphism studies on NIHL have been performed extensively. However, the proteomic profiles in the cochleae of mice suffering noise damage remain unclear. The goal of this current study was to perform a comprehensive investigation on characterizing protein expression changes in the cochlea based on a mouse model of NIHL using tandem mass tag (TMT)-labeling quantitative proteomics, and to reveal the potential biomarkers and pathogenesis of NIHL. Male C57BL/6J mice were exposed to noise at 120 dB SPL for 4 h to construct the NIHL mouse model. The levels of MDA and SOD, and the production of proinflammatory cytokines including TNF-α and IL-6 in the mice cochleae, were determined using chemical colorimetrical and ELISA kits. Moreover, differentially expressed proteins (DEPs) were validated using Western blotting. The mouse model showed that the ABR thresholds at frequencies of 4, 8, 12, 16, 24 and 32 kHz were significantly increased, and outer hair cells (HCs) showed a distinct loss in the noise-exposed mice. Proteomics analysis revealed that 221 DEPs were associated with NIHL. Bioinformatics analysis showed that a set of key inflammation and autophagy-related DEPs (ITGA1, KNG1, CFI, FGF1, AKT2 and ATG5) were enriched in PI3K/AKT, ECM-receptor interaction, and focal adhesion pathways. The results revealed that the MDA level was significantly increased, but the activity of SOD decreased in noise-exposed mice compared to the control mice. Moreover, TNF-α and IL-6 were significantly increased in the noise-exposed mice. Western blotting revealed that the expression levels of ITGA1, KNG1, and CFI were upregulated, but FGF1, AKT2, and ATG5 were significantly downregulated in noise-exposed mice. This study provides new scientific clues about the future biomarkers and pathogenesis studies underlying NIHL. Furthermore, the findings suggest that the validated DEPs may be valuable biomarkers of NIHL, and inflammation and autophagy may be pivotal mechanisms that underlie NIHL.

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Association of Plasma Metabolomic Biomarkers With Persistent Tinnitus

Zeleznik

Welling

Stanković

et al. 2023

JAMA Otolaryngol Head Neck Surg

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ImportancePersistent tinnitus is common, disabling, and difficult to treat.ObjectiveTo evaluate the association between circulating metabolites and persistent tinnitus.Design, Setting, and ParticipantsThis was a population-based case-control study of 6477 women who were participants in the Nurses’ Health Study (NHS) and NHS II with metabolomic profiles and tinnitus data. Information on tinnitus onset and frequency was collected on biennial questionnaires (2009-2017). For cases, metabolomic profiles were measured (2015-2021) in blood samples collected after the date of the participant’s first report of persistent tinnitus (NHS, 1989-1999 and 2010-2012; NHS II, 1996-1999). Data analyses were performed from January 24, 2022, to January 14, 2023.ExposuresIn total, 466 plasma metabolites from 488 cases of persistent tinnitus and 5989 controls were profiled using 3 complementary liquid chromatography tandem mass spectrometry approaches.Main Outcomes and MeasuresLogistic regression was used to estimate odds ratios (ORs) of persistent tinnitus (per 1 SD increase in metabolite values) and 95% CIs for each individual metabolite. Metabolite set enrichment analysis was used to identify metabolite classes enriched for associations with tinnitus.ResultsOf the 6477 study participants (mean [SD] age, 52 [9] years; 6477 [100%] female; 6121 [95%] White individuals) who were registered nurses, 488 reported experiencing daily persistent (≥5 minutes) tinnitus. Compared with participants with no tinnitus (5989 controls), those with persistent tinnitus were slightly older (53.0 vs 51.8 years) and more likely to be postmenopausal, using oral postmenopausal hormone therapy, and have type 2 diabetes, hypertension, and/or hearing loss at baseline. Compared with controls, homocitrulline (OR, 1.32; (95% CI, 1.16-1.50); C38:6 phosphatidylethanolamine (PE; OR, 1.24; 95% CIs, 1.12-1.38), C52:6 triglyceride (TAG; OR, 1.22; 95% CIs, 1.10-1.36), C36:4 PE (OR, 1.22; 95% CIs, 1.10-1.35), C40:6 PE (OR, 1.22; 95% CIs, 1.09-1.35), and C56:7 TAG (OR, 1.21; 95% CIs, 1.09-1.34) were positively associated, whereas α-keto-β-methylvalerate (OR, 0.68; 95% CIs, 0.56-0.82) and levulinate (OR, 0.60; 95% CIs, 0.46-0.79) were inversely associated with persistent tinnitus. Among metabolite classes, TAGs (normalized enrichment score [NES], 2.68), PEs (NES, 2.48), and diglycerides (NES, 1.65) were positively associated, whereas phosphatidylcholine plasmalogens (NES, −1.91), lysophosphatidylcholines (NES, −2.23), and cholesteryl esters (NES,−2.31) were inversely associated with persistent tinnitus.Conclusions and RelevanceThis population-based case-control study of metabolomic profiles and tinnitus identified novel plasma metabolites and metabolite classes that were significantly associated with persistent tinnitus, suggesting that metabolomic studies may help improve understanding of tinnitus pathophysiology and identify therapeutic targets for this challenging disorder.

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Plasma metabolomic profiling in workers with noise-induced hearing loss: a pilot study

Cited by 17 publications

References 66 publications

Metabolomic Studies in Inner Ear Pathologies

Metabolomic Studies in Inner Ear Pathologies

TMT-Based Quantitative Proteomics Reveals Cochlear Protein Profile Alterations in Mice with Noise-Induced Hearing Loss

Association of Plasma Metabolomic Biomarkers With Persistent Tinnitus

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