Plasma modified PLA electrospun membranes for actinorhodin production intensification in Streptomyces coelicolor immobilized-cell cultivations

Scaffaro, Roberto; Lopresti, Francesco; Sutera, Alberto; Botta, Luigi; Fontana, Rosa; Gallo, Giuseppe

doi:10.1016/j.colsurfb.2017.05.060

Cited by 43 publications

(22 citation statements)

References 56 publications

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“…The increase of the hydrophilicity of plasma-treated PLA scaffolds enhance the affinity of the surface to support the aloe vera coating in an effective way. The functional groups created during plasma treatment of PLA are mainly hydroxyl and carbonyl ones [13,41]. These groups are not reactive with the functional groups in the structure of the polysaccharides in aloe vera extracts (mainly hydroxyl and acetyl) [42].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering

et al. 2020

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3D-printed polylactic acid (PLA) scaffolds have been demonstrated as being a promising tool for the development of tissue-engineered replacements of bone. However, this material lacks a suitable surface chemistry to efficiently interact with extracellular proteins and, consequently, to integrate into the surrounding tissue when implanted in vivo. In this study, aloe vera coatings have been proposed as a strategy to improve the bioaffinity of this type of structures. Aloe vera coatings were applied at three different values of pH (3, 4 and 5), after treating the surface of the PLA scaffolds with oxygen plasma. The surface modification of the material has been assessed through X-ray photoelectron spectroscopy (XPS) analysis and water contact angle measurements. In addition, the evaluation of the enzymatic degradation of the structures showed that the pH of the aloe vera extracts used as coating influences the degradation rate of the PLA-based scaffolds. Finally, the cell metabolic activity of an in vitro culture of human fetal osteoblastic cells on the samples revealed an improvement of this parameter on aloe vera coated samples, especially for those treated at pH 3. Hence, these structures showed potential for being applied for bone tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering

et al. 2020

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“…Many investigations reported scaffold fabrication of synthetic and biodegradable polymers such as poly(lactic-co-glycolicacid) (PLGA) by electrospinning [13]. Wang et al [14], combined PLGA with other biomaterials, while Scaffaro et al in [15], subjected the same material to O 2 plasma treatment.…”

Section: Introductionmentioning

confidence: 99%

Graphene Nanoplatelet- and Hydroxyapatite-Doped Supramolecular Electrospun Fibers as Potential Materials for Tissue Engineering and Cell Culture

Kostopoulos

Κοτρώτσος

Fouriki

2019

IJMS

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Porous and fibrous artificial extracellular matrices (ECM) called scaffolds are considered to be promising avenues of research in the field of biomedical engineering, including tissue fabrication through cell culture. The current work deals with the fabrication of new matrix-type scaffolds through electrospinning, in order to support future three-dimensional tissue formation. The selected material for the fabrication of these scaffolds was a supramolecular polymer (SP) that is based on ureiodypyrimidone hydrogen bonding units (UPy). More precisely, pure SP and modified electrospun scaffolds with (a) graphene nanoplatelets (GNPs), (b) hydroxyapatite (HA), and (c) a mixture of both were fabricated for the needs of the current study. The aim of this work is to engineer and to characterize SP electrospun scaffolds (with and without fillers) and study whether the introduction of the fillers improve the physical and mechanical properties of them. The obtained results indicate that doping the SP scaffolds with GNPs led to improved apparent mechanical properties while HA seems to slightly deteriorate them. For all cases, doping provided thinner fibers with a more hydrophilic surface. Taking together, these types of SP scaffolds can be further studied as potential candidate for cell culture.

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“…In order to create hierarchical structure with more rough surfaces, a number of methods are developed to manufacture superhydrophobic surfaces, including templates, chemical vapor deposition, laser irradiation, and so forth . Although these methods are effective, they require high cost of machinery and precise conditions.…”

Section: Introductionmentioning

confidence: 99%

Preparation of hierarchically structured PCL superhydrophobic membrane via alternate electrospinning/electrospraying techniques

Zhang

Wang

Zhang

et al. 2019

J Polym Sci B Polym Phys

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Superhydrophobic polycaprolactone (PCL) membranes with hierarchical structure were fabricated via alternate electrospinning/electrospraying techniques. Electrospun PCL/methyl silicone oil (PCL/MSO) nanofibers were employed as substrate. PCL/MSO‐PCL microspheres (PCL/MSO‐PCLMS) hierarchical membrane was prepared via electrosprayed PCLMS as an additional layer on the substrate. Field emission scanning electron microscopy images showed the formation of hierarchical PCL/MSO‐PCLMS membranes. Compared to pure PCL fibers substrate (120 ± 1.3°), the water contact angle (WCA) of MSO‐modified PCL membrane was 142 ± 0.7°. The most interesting observation was that the WCA of PCLMS without any modification could be achieved to 146 ± 2.8°. On this basis, PCL/MSO‐PCLMS hierarchical membrane possessed superhydrophobic surface with 150 ± 0.6° of WCA. The excellent surface roughness and air‐pocket capacity of hierarchical membranes would make the membranes more hydrophobic. The maximum oil (n‐hexane) adsorption capacity of PCL/MSO‐PCLMS membrane was 32.53 g g−1. Oil–water separation efficiencies of the superhydrophobic membranes were all higher than 99.93% after 10 cycles. The hierarchically structured PCL superhydrophobic membranes indicate the potential applications of environmentally friendly biopolymers as separation membranes. © 2019 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2019, 57, 421–430

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Plasma modified PLA electrospun membranes for actinorhodin production intensification in Streptomyces coelicolor immobilized-cell cultivations

Cited by 43 publications

References 56 publications

Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering

Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering

Graphene Nanoplatelet- and Hydroxyapatite-Doped Supramolecular Electrospun Fibers as Potential Materials for Tissue Engineering and Cell Culture

Preparation of hierarchically structured PCL superhydrophobic membrane via alternate electrospinning/electrospraying techniques

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