Plasma postheparin diamine oxidase. Sensitive provocative test for quantitating length of acute intestinal mucosal injury in the rat.

Luk, Gordon D.; Bayless, Theodore M.; Baylin, Stephen B.

doi:10.1172/jci110881

Cited by 115 publications

(63 citation statements)

References 17 publications

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“…The level of sICAM-1 in the serum was detected with an ELISA, the plasma level of D-lactate was determined, after the plasma was deproteinized with perchloric acid, using enzyme-coupled UV-spectrophotometry [8] ; the serum DAO was determined by spectrophotometry as by Luk et al [2] ; the number of white blood cells (WBC) was detected routinely.…”

Section: Major Analysis Apparatus: 721 Spectrophotometer (Shanghai Somentioning

confidence: 99%

“…In cases where the intestinal mucosal epithelial cells and barrier function are damaged, the release of DAO is increased, and DAO enters the extracellular space, lymphatic vessels and bloodstream, thus increasing the level of DAO in the plasma. As the activity of DAO is stable, its concentration in the blood can reflect the damage and restoration of the intestinal cavity [2] . Overactivation of leukocytes is an important pathological process of IBD.…”

Section: Introductionmentioning

confidence: 99%

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Soluble intercellular adhesion molecule-1, D-lactate and diamine oxidase in patients with inflammatory bowel disease

Song¹,

Lv²,

Zhang³

et al. 2009

WJG

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AIM:To study the levels of serum soluble intercellular adhesion molecule-1 (sICAM-1), plasma D-lactate and diamine oxidase (DAO) in patients with inflammatory bowel disease (IBD), and the potential clinical significance. METHODS:Sixty-nine patients with IBD and 30 healthy controls were included in this study. The concentration of sICAM-1 was detected with enzyme-linked immunosorbent assay, the level of D-lactate and DAO was measured by spectroscopic analysis, and the number of white blood cells (WBC) was determined by routine procedure. RESULTS:The levels of sICAM-l, DAO, and WBC in IBD patients were significantly higher than those in the control group (P < 0.01). sICAM-l in IBD patients was found to be closely related to the levels of DAO and D-lactate (212.94 ± 69.89 vs 6.35 ± 2.35, P = 0.000), DAO 212.94 ± 69.89 vs 8.65 ± 3.54, P = 0.000) and WBC (212.94 ± 69.89 vs 7.40 ± 2.61, P = 0.000), but no significant difference was observed between patients with ulcerative colitis and patients with Crohn's disease. The post-treatment levels of sICAM-l, D-lactate and WBC were significantly lower than before treatment (sICAM-l 206.57 ± 79.21 vs 146.21 ± 64.43, P = 0.000), (D-lactate 1.46 ± 0.94 vs 0.52 ± 0.32, P = 0.000) and (WBC 7.24 ± 0.2.33 vs 5.21 ± 3.21, P = 0.000).CONCLUSION: sICAM-1, D-lactate and DAO are closely related to the specific conditions of IBD, and thus could be used as a major diagnostic index.

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Section: Major Analysis Apparatus: 721 Spectrophotometer (Shanghai Somentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Soluble intercellular adhesion molecule-1, D-lactate and diamine oxidase in patients with inflammatory bowel disease

Song¹,

Lv²,

Zhang³

et al. 2009

WJG

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“…In Tiers tu dien konn te eine sig ni fi kan te Kor re lati on zwi schen der DAO-Ak ti vi tät im Plas ma und der in den Zot ten der Dünn darm schleimhaut ge zeigt wer den [27,28,29,30]. Al lerdings wie sen Wol ve camp und Mit ar bei ter [17] da rauf hin, dass die Se rum-His ta minkon zen tra ti on in sehr ge rin gen Men gen im Kreis lauf prä sent ist.…”

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Histaminintoleranz: Wie sinnvoll ist die Bestimmung der Diaminoxidase-Aktivität im Serum in der alltäglichen klinischen Praxis?

Töndury¹,

Wüthrich²,

Schmid‐Grendelmeier³

et al. 2008

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(2008)His ta mi nin to le ranz: Wie sinn voll ist die Be stim mung der Di amin oxi da se-Ak tivi tät im Se rum in der all tägli chen kli nischen Praxis?Hin ter grund: Eine Nah rungs mit tel un verträg lich keit kann durch ei nen ver min der ten en zy ma ti schen His ta min ab bau auf grund einer ver rin ger ten Ak ti vi tät der Di amin oxi da se (DAO) in du ziert sein (His ta mi nin to le ranz (HIT)). Ziel set zung: Ziel der vor lie gen den Stu die war es he raus zu fin den, ob die DAOAktivität im Se rum bei Pa tien ten, de ren Vorge schich te eine HIT ver mu ten lässt, im Vergleich zu Pa tien ten ohne eine Vor ge schich te mit HIT sig ni fi kant ver rin gert ist. Me tho den: Un ter sucht wur den 61 Pa tien ten und 20 Kontrol len. Bei 26 Pa tien ten (schwere HIT) lag eine Vor ge schich te mit min de stens zwei ty pischen HIT-Symp to men in Zu sam men hang mit der Auf nah me von min de stens zwei his tamin rei chen Nah rungs mit teln vor. Bei 35 Patien ten (mä ßi ge HIT) war nach der Auf nah me von min de stens ei nem his ta min rei chen Nahrungs mit tel min de stens ein HIT-Symp tom auf ge tre ten. Die 20 ge sun den Kon trol len zeig ten kei ner lei HIT-Symp to me. Die Pa tienten wur den zur Art der kli ni schen Symp to me und zum Zu sam men hang der Symp to me mit der Auf nah me der Nah rungs mit tel be fragt. Die DAO-Aktivität im Se rum wur de mit tels ELI SA ana ly siert. Er geb nis se: Es konn ten kei ne Un ter schie de be züg lich der DAO-Konzen tra ti on im Se rum zwi schen den Grup pen fest ge stellt wer den. Schluss fol ge rung: Ba sierend auf der Ana mne se mit al ler gie ähn li chen Symp to men nach Auf nah me von histaminreichen Nah rungs mit teln bie tet die Bestim mung der DAO-Ak ti vi tät im Se rum in der all täg li chen kli ni schen Pra xis kei ne Hilfe stel lung für die Di ag no se ei ner HIT.His ta mine in tol er ance: Is the de ter mina tion of diamine oxidase ac tiv ity in the serum use ful in rou tine clin i cal prac tice?Back ground: An in tol er ance to food might be in duced by an im paired en zy matic his ta mine deg ra da tion due to a de fi ciency of diamine oxidase (DAO) ac tiv ity (His ta mine in tol er ance [HIT]). Ob jec tive: The aim of the study was to in ves ti gate if pa tients his to ries, that are sug ges tive to HIT have a sig nif i cant re duced se rum DAO ac tiv ity com pared to patients with out his tory of HIT. Meth ods: 61 patients and 20 con trols were stud ied. 26 patients (strong HIT) had a his tory of at least two typ i cal symp toms of HIT with re la tion to the in take of at least two his ta mine rich foods. 35 pa tients (mod er ate HIT) had a his tory of at least one symp tom af ter the in take of at least one his ta mine rich food (but not both). 20 healthy vol un teers (con trol) did not have any symp toms of HIT. Pa tients were in ter viewed on type of clin i cal symp toms and the re la tion of symp toms to food in ges tion. Sera were ana lysed for DAO ac tiv ity by an ELISA test. Re sults: No dif fer ence of se rum DAO lev els could be found be tween groups o...

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“…In humans and rodents, DAO is found in various tissues, with small intestinal mucosa showing the highest enzymatic activity [5]. Furthermore, plasma DAO activity increases in parallel with DAO activity in the villi of the small intestinal mucosa in maturing rats [6,7] and correlates with the severity of small intestinal mucosal lesions induced by anti-cancer drugs [8]. In addition, serum levels of DAO activities seem to be a reliable indicator of intestinal mucosal integrity and reflect quantitative changes in the small bowel mucosal mass [6,9,10].…”

Section: Introductionmentioning

confidence: 99%

Plasma Diamine Oxidase Activity Is a Useful Biomarker for Evaluating Gastrointestinal Tract Toxicities during Chemotherapy with Oral Fluorouracil Anti-Cancer Drugs in Patients with Gastric Cancer

Namikawa¹,

Fukudome²,

Kitagawa³

et al. 2012

Oncology

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Objectives: Diamine oxidase (DAO) is an enzyme that catalyzes oxidation and is highly active in the mature upper villus cells of the intestinal mucosa. This study sought to evaluate plasma DAO activities during adjuvant chemotherapy in patients with gastric cancer. Methods: We investigated 20 patients with gastric cancer who were treated with oral fluorouracil anti-cancer drugs as adjuvant chemotherapy. Plasma DAO activity was measured in all patients before chemotherapy and at 2, 4 and 6 weeks after the start of chemotherapy, and quality of life was evaluated simultaneously. Results: The median DAO activity after 4 weeks of chemotherapy was significantly decreased compared to the pre-chemotherapy levels (6.6 vs. 7.5 U/l; p = 0.038). The changes in the rate of DAO activity at 2 and 6 weeks following the start of chemotherapy in patients with gastrointestinal tract toxicity were significantly lower than in those without toxicity (p =0.021 and 0.047, respectively). The patient cohort showed a slightly positive correlation between DAO activity and global health status and a negative correlation between DAO activity and appetite loss. Conclusions: Plasma DAO activities may be useful for monitoring and evaluating gastrointestinal tract toxicities induced by adjuvant chemotherapy with oral fluorouracil in patients with gastric cancer.

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Plasma postheparin diamine oxidase. Sensitive provocative test for quantitating length of acute intestinal mucosal injury in the rat.

Cited by 115 publications

References 17 publications

Soluble intercellular adhesion molecule-1, D-lactate and diamine oxidase in patients with inflammatory bowel disease

Soluble intercellular adhesion molecule-1, D-lactate and diamine oxidase in patients with inflammatory bowel disease

Histaminintoleranz: Wie sinnvoll ist die Bestimmung der Diaminoxidase-Aktivität im Serum in der alltäglichen klinischen Praxis?

Plasma Diamine Oxidase Activity Is a Useful Biomarker for Evaluating Gastrointestinal Tract Toxicities during Chemotherapy with Oral Fluorouracil Anti-Cancer Drugs in Patients with Gastric Cancer

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