Plasma protein thiols: an early marker of oxidative stress in asthma and chronic obstructive pulmonary disease

Abstract

Chronic obstructive pulmonary disease (COPD) and asthma are both characterized by heterogeneous chronic airway inflammation and obstruction as well as oxidative stress (OS). However, it is unknown whether OS occurs in early disease and how to best assess its presence. Plasma OS markers (TBARS, PSH, taurine, GSH, ergothioneine and paraoxonase 1 activity) and lung function tests were measured in patients with mild stable asthma (n = 24) and mild stable COPD (n = 29) and in age- and sex-matched controls. Forced expiratory volume in 1 s (FEV1 ) was associated with age both in patients and control groups. By contrast, FEV1 was positively correlated with PSH only in COPD (ρ = 0·49, P = 0·007). In multiple logistic regression analysis, lower PSH was the only OS marker independently associated with increased odds of both asthma (OR = 0·32, 95% CI 0·13-0·78, P = 0·01) and COPD (OR = 0·50, 95% CI 0·26-0·95, P = 0·03). These findings suggest that proteins -SH are a sensitive OS marker in early COPD and asthma.

The Effect of <b><i>Allium cepa</i></b> Extract on Lung Oxidant, Antioxidant, and Immunological Biomarkers in Ovalbumin-Sensitized Rats

“…The role of thiol, an early marker of oxidative stress in asthma, in the reduction of SOD [29] and CAT activity in asthmatic patients was stressed previously [30], which was in agreement with the changes observed in sensitized animals in this study. In addition, similar to the findings of the present study, upregulation of the release of Th2 cytokines (mainly IL-4, IL-5, and IL-13) and downregulation of the release of IFN-γ were also noticed in asthmatic patients [30].…”

Dietary flavonoids advance timing of moult but do not affect redox status of juvenile blackbirds (Turdus merula)

“…Protein carbonylation constitutes a mostly irreversible type of severe oxidative damage that often results in the loss of protein structure and/or functional efficiency (Dalle-Donne et al, 2003;Barreiro and Hussain, 2010;Barreiro, 2014 Conversely, protein thiols are highly susceptible to oxidation and a decrease of plasma PSH concentration may indicate oxidative stress (Rossi et al, 2009;Zinellu et al, 2016).…”

Circulating biomarkers of oxidative stress in chronic obstructive pulmonary disease: a systematic review

“…We investigated MDA levels considering only mild COPD, finding no differences between patients and controls [55] (Table 1). Some authors have analyzed MDA measuring TBA-MDA adduct with high performance liquid chromatography (HPLC) rather than spectrophotometer and finding a significant increase of this biomarker in COPD compared to healthy controls [56, 57] (Table 1).…”

“…This compound is reduced by free thiols in an exchange reaction, forming a mixed disulphide and releasing one molecule of 5-thionitrobenzoic acid, which can be measured at 412 nm [91]. This method of detection has been used in several studies to measure the content of both protein and non-protein SH groups in the peripheral blood of COPD patients.○ Protein SH groups : in this regard a significant decrease of this biomarker has been described in COPD compared to healthy controls [29, 30, 36, 92] and even in mild COPD [55] (Table 1). Some authors have investigated this biomarker in the different stages of severity of COPD finding no differences [29, 36].…”

“…A significant decrease in non-protein SH groups, mainly in the form of reduced GSH, has been found in many of the examined studies [28, 34, 40, 41, 43, 45, 53] (Table 1). No differences have been found considering only mild COPD [55] (Table 1). When investigated in the different stages of COPD severity, no difference has been found, either [40] (Table 1).…”