Plasma renin activity: An early marker of progressive renal disease in posterior urethral valves

Bajpai, Minu; Singh, Amit

doi:10.4103/0971-9261.121114

Cited by 11 publications

(9 citation statements)

References 18 publications

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“…[13] Similarly, we found that the mean GFR at presentation in patients who did not develop CRF was 70.39 ± 24.03 ml/min/1.73 m 2 compared to 39.55 ± 19.97 in patients who ultimately developed CRF. This difference was statistically significant ( P < 0.05).…”

Section: Discussionsupporting

confidence: 55%

Posterior urethral valve: Prognostic factors and renal outcome

Bhadoo

Bajpai

Panda

2014

J Indian Assoc Pediatr Surg

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Objective:The aim was to study the outcome of posterior urethral valve (PUV) cases treated by stepladder protocol and the prognostic factors affecting the outcome.Materials and Methods:Hospital records of all PUV patients treated by stepladder protocol between January 1992 and December 2013 were reviewed. The studied parameters were: Age at presentation, serum creatinine, types of surgical intervention, vesicoureteral reflux (VUR) on initial voiding cystourethrogram (VCUG), renal cortical scars, plasma renin activity (PRA), and glomerular filtration rate (GFR).Results:Of 396 PUV patients treated during the study period, 152 satisfied study criteria. The age at presentation ranged from 2 days to 15 years (mean 31.3 months). The mean follow-up period was 5 years (range: 2-18 years). Primary endoscopic valve ablation was the most common initial procedure. Chronic renal failure was seen in 42.7% patients at the last follow-up. Serum creatinine at presentation, initial PRA levels, initial GFR, and PRA levels at last follow-up were significant predictors of final renal outcome. Age at presentation (<1 vs. >1 year), presence/absence of VUR on initial VCUG and renal cortical scars had no significant correlation with ultimate renal function.Conclusion:Our study confirms the high prognostic significance of initial serum creatinine, PRA levels and GFR in cases with PUV. PRA also holds promise in long-term follow-up of these patients as a marker of progressive renal damage.

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Section: Discussionsupporting

confidence: 55%

Posterior urethral valve: Prognostic factors and renal outcome

Bhadoo

Bajpai

Panda

2014

J Indian Assoc Pediatr Surg

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“…Ltd. PRA values were compared with the reference value corresponding to the age. Normal PRA values were: < 1 year = 4–8 ng/mL/h; 1–3 years = 1–9 ng/mL/h; 3–6 years = 1–5 ng/mL/h; 6–13 years = 1.4–2.6 ng/mL/h) [5]. …”

Section: Methodsmentioning

confidence: 99%

Clinico-Biochemical Profile of Children with Congenital Anomalies of the Kidney and Urinary Tract: A Cross-Sectional Study

et al. 2018

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Background: Congenital anomalies of the kidney and urinary tract (CAKUT) are a group of disorders responsible for the majority of pediatric end-stage renal disease cases. There are only a few studies on CAKUT. Objectives: A study was conducted to determine the clinical and biochemical profile of children with CAKUT and to estimate the prevalence and the factors associated with hypertension in CAKUT. Methods: A cross-sectional study was conducted in a tertiary center for 18 months from March 2014 to August 2015. Demographic data were recorded, and clinical examination including blood pressure measurement was performed. Various biochemical parameters including plasma renin activity (PRA), urinary beta-2-microglobulin (B2M), and microalbuminuria were evaluated. Results: A total of 81 patients with CAKUT were studied. Twenty-two (27%) patients were underweight, 4 (5%) patients were stunted, and 26 (32%) were both underweight and stunted. Children with bilateral disease had a higher incidence of underweight (21/44 vs. 8/37; p = 0.04; 95% CI; Fisher exact test), and both underweight and stunted (25/44 vs. 10/37; p = 0.006; 95% CI; Fisher exact test) compared to children with unilateral disease. Hypertension was found in 27% cases. No association was found between hypertension and PRA, BM2, or microalbuminuria in our study. PRA was inversely proportional to the estimated glomerular filtration rate (eGFR) (Pearson test; 95% CI; p = 0.006). Conclusions: Bilateral disease in CAKUT was significantly associated with poor somatic growth. PRA was inversely proportional to eGFR. The prevalence of hypertension was higher in children with CAKUT than in normal children and is possibly multifactorial as it was not associated with elevated PRA, B2M, or microalbuminuria.

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“…Plasma renin activity (PRA) measurement has gained recognition as a substitute of RAAS activity evaluation [3, 4, 5]. PRA has been suggested to be a predictor of the increased risk of cardiovascular diseases [6, 7, 8, 9, 10] and renal dysfunction [11, 12, 13].…”

Section: Introductionmentioning

confidence: 99%

Establishing a novel assay system for measuring renin concentration using cost effective recombinant ovine angiotensinogen

Akther

Nabi

Ohno

et al. 2019

Heliyon

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Background Plasma renin can predict future cardiovascular events as well as the prevalence of chronic renal disease in hypertensive subjects. Ovine angiotensinogen (oANG) is a better substrate for measuring renin concentration through activity assay. Recombinant oANG expressed in Escherichia coli cells can be utilized as the substrate while measuring plasma renin. We aim to establish an immunoassay for measuring renin concentration at picomolar level using recombinant oANG. Material and methods Recombinant oANG was expressed in E. coli cells and purified to homogeneity. Various concentrations (0–1.5 pM) of recombinant human renin standard were prepared and incubated with recombinant oANG. Renin activity was determined by angiotensin-I specific enzyme-linked immunosorbent assay. Results About 4.5 mg of purified recombinant oANG was obtained from 0.5 L of E. coli culture. The Michaelis constant and turnover number of human renin with recombinant oANG were 0.16 μM and 0.51 s −1 , respectively. A linear relationship was obtained when renin activity was plotted as a function of renin concentration using recombinant oANG as the renin substrate. Picomolar amounts of renin can be measured from known renin activity using this method. Conclusion This study established a novel assay system for measuring renin at picomolar level using cost effective recombinant oANG.

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Plasma renin activity: An early marker of progressive renal disease in posterior urethral valves

Cited by 11 publications

References 18 publications

Posterior urethral valve: Prognostic factors and renal outcome

Posterior urethral valve: Prognostic factors and renal outcome

Clinico-Biochemical Profile of Children with Congenital Anomalies of the Kidney and Urinary Tract: A Cross-Sectional Study

Establishing a novel assay system for measuring renin concentration using cost effective recombinant ovine angiotensinogen

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