Plasma treatment as an effective tool for crosslinking of electrospun fibers

Molnár, Kristóf; Jozsa, Benjamin; Barczikai, Dóra; Krisch, Enikő; Puskás, Judit E.; Jedlovszky-Hajdú, Angéla

doi:10.1016/j.molliq.2020.112628

Cited by 16 publications

(12 citation statements)

References 33 publications

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“…FTIR detects several tens of nanometers inside a sample even in the ATR mode, leading to a much bigger contribution of the bulk than the surface to the IR signal [ 32 ]. In contrast, X-ray photoelectron spectroscopy (XPS) at 90° probes ~10 nm from the surface and shows changes upon plasma treatment [ 26 ]. The survey spectra of PSI-AAm and PSI-AAmp had three major peaks representing C, N, and O atoms in both samples (not shown).…”

Section: Resultsmentioning

confidence: 99%

“…The synthesis of allylamine-modified polysuccinimide (PSI-AA) was published previously [ 16 , 26 ]. In this study, allylamine-modified PSI with a theoretical grafting degree (GD) of 2 (every second repeat unit had one AA attached) was used.…”

Section: Methodsmentioning

confidence: 99%

“…The fibrous membrane (PSI-AAm) was then removed from the collector, and small ~5 mm diameter disks were cut for the cell culture studies. The disks were placed in a Petri dish with a perforated Petri dish cover and were exposed to low-pressure non-equilibrium air plasma treatment using a Zepto benchtop plasma reactor (Diener Electronics GMBH, Ebhausen, Germany) [ 26 ]. The low frequency (40 kHz) plasma generator surrounded a 105 mm diameter, 300 mm depth tubular reactor with the samples.…”

Section: Methodsmentioning

confidence: 99%

“…These fibers were found to be stable in water in vitro, although disulfide linkages can disintegrate under physiological conditions due to the presence of reducing agents in body fluids [ 25 ]. In the third case, crosslinking of allylamine-modified PSI-based fiber mat (PSI-AAm) was induced by plasma treatment [ 26 ]. The crosslinked structure was confirmed by the fact that the plasma-treated PSI-AAm (PSI-AAmp) did not dissolve in dimethyl sulfoxide, which is a good solvent for the non-crosslinked (non-plasma-treated) PSI-AAm.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the Cytotoxicity of Electrospun Polysuccinimide-Based Fiber Mats

et al. 2020

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This study investigated cell viability in the presence of allylamine-modified and plasma-treated electrospun polysuccinimide fiber mats (PSI-AAmp). Low pressure non-equilibrium plasma was used for crosslinking the PSI-AAm. Comparison of FTIR and XPS analyses demonstrated that crosslinking occurred on the surface of the samples. Cell viability was investigated using the MG-63 osteosarcoma cell line and WST-1 viability reagent. Since PSI hydrolyzes to poly(aspartic acid) (PASP), PASP was used in addition to the regular controls (cells only). Phase contrast showed normal morphology in all cases at 24 h; however, in the presence of PSI-AAmp at 72 h, some rounded, dead cells could also be seen, and proliferation was inhibited. Since proliferation in the presence of PASP alone was not inhibited, the cause of inhibition was not the final product of the hydrolysis. Further investigations will be carried out to pinpoint the cause.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigation of the Cytotoxicity of Electrospun Polysuccinimide-Based Fiber Mats

et al. 2020

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“…Polysuccinimide was first modified with allylamine (PSI-AA) to attach a reactive allyl groups to the polymer chain. PSI-AA meshes were created by electrospinning followed by low pressure plasma treatment to allow the allylamine groups to establish crosslinks in the meshes [128].…”

Section: Plasma Treatmentmentioning

confidence: 99%