Plasmid and restriction endonuclease patterns in Pasteurella multocida isolated from a swine pyramid

Rúbies, Xavier; Casal, Jordi Domènech; Pijoan, Carlos

doi:10.1016/s0378-1135(01)00440-0

Cited by 10 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of 125 isolates associated with pneumonia, 101 (81 %) were of capsular type A, 20 (16 %) were of capsular type D, two (1 %) were of capsular type F and two (1 %) were untypable. The higher proportion of capsular type A strains associated with pneumonia, compared with capsular type D isolates, is in agreement with previous studies (Pijoan et al, 1983(Pijoan et al, , 1984Rubies et al, 2002;Zhao et al, 1992;. Capsular type F and untypable strains are uncommon in pigs, but are often isolated from avian hosts Wilson et al, 1993 (Eamens et al, 1988;Foged et al, 1988;Gardner et al, 1994;Lariviere et al, 1992;Sakano et al, 1992).…”

Section: Discussionsupporting

confidence: 87%

“…In contrast to isolates associated with PAR, P. multocida strains isolated from pneumonic lungs are usually non-toxigenic and of capsular type A (Pijoan et al, 1983(Pijoan et al, , 1984Rubies et al, 2002;Zhao et al, 1992 Despite the obvious differences between pneumonia and PAR and their association with P. multocida strains of different capsular types and toxin status, very little is known about the relationships of isolates responsible for each of these diseases (Djordjevic et al, 1998). A number of different methods such as bacteriophage typing, plasmid profiling, restriction endonuclease analysis, ribotyping and analysis of outer-membrane proteins (OMPs) have been used to examine diversity among P. multocida strains associated with either PAR (Gardner et al, 1994;Fussing et al, 1999;Harel et al, 1990;Donnio et al, 1999;Nielsen & Rosdahl, 1990;Lugtenberg et al, 1984;Vasfi Marandi & Mittal, 1995;Bowles et al, 2000) or pneumonia (Rubies et al, 2002;Zhao et al, 1992;Blackall et al, 2000). However, there have been very few comparative studies of P. multocida isolates derived from both PAR and pneumonia (Djordjevic et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Non-toxigenic P. multocida strains are not usually associated with PAR and confirmation of toxin production is important for the diagnosis and control of the disease (Bowersock et al, 1992). P. multocida is also frequently isolated from the lungs of pneumonic pigs and is thought to play a central role in porcine pneumonic pasteurellosis (Pijoan et al, 1983(Pijoan et al, , 1984Pijoan, 1992;Rubies et al, 2002;Zhao et al, 1992). Prior infection with other micro-organisms such as pseudorabies virus and Mycoplasma hyopneumoniae predisposes pigs to secondary infection with P. multocida (Ciprian et al, 1988;.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to isolates associated with PAR, P. multocida strains isolated from pneumonic lungs are usually non-toxigenic and of capsular type A (Pijoan et al, 1983(Pijoan et al, , 1984Rubies et al, 2002;Zhao et al, 1992). However, a small proportion of lung isolates are toxigenic and/or possess capsular type D (Pijoan et al, 1984;Rubies et al, 2002;Choi et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…A number of different methods such as bacteriophage typing, plasmid profiling, restriction endonuclease analysis, ribotyping and analysis of outer-membrane proteins (OMPs) have been used to examine diversity among P. multocida strains associated with either PAR (Gardner et al, 1994;Fussing et al, 1999;Harel et al, 1990;Donnio et al, 1999;Nielsen & Rosdahl, 1990;Lugtenberg et al, 1984;Vasfi Marandi & Mittal, 1995;Bowles et al, 2000) or pneumonia (Rubies et al, 2002;Zhao et al, 1992;Blackall et al, 2000). However, there have been very few comparative studies of P. multocida isolates derived from both PAR and pneumonia (Djordjevic et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

Davies

MacCorquodale

Baillie

et al. 2003

Journal of Medical Microbiology

104

View full text Add to dashboard Cite

One hundred and fifty-eight porcine strains of Pasteurella multocida, recovered primarily from cases of pneumonic pasteurellosis or progressive atrophic rhinitis (PAR) in England and Wales, were characterized by determination of their capsular types, presence or absence of the toxA gene and molecular mass heterogeneity of the heat-modifiable (OmpA) and porin (OmpH) proteins. Eighteen groups (clones) of strains were identified on the basis of specific combinations of capsular type, toxA status and outer-membrane protein (OMP)-type. The data provided evidence that different subpopulations of P. multocida are responsible for pneumonia and PAR in pigs. The majority (88 %) of cases of pneumonia were associated exclusively with non-toxigenic capsular type A strains of OMP-types 1.1, 2.1, 3.1 and 5.1 and capsular type D isolates of OMP-type 6.1. These strains were recovered from widespread geographical locations within England and Wales over a 12-year period and represented mostly single sporadic cases. The association of a small number of P. multocida variants with the majority of cases of porcine pneumonia suggests that these strains are not opportunistic pathogens of low virulence but represent primary pathogens with a relatively high degree of virulence. In contrast, the majority (76 %) of cases of PAR were associated with toxAcontaining capsular type D strains of OMP-type 4.1 and capsular type A and D strains of OMP-type 6.1. Toxigenic capsular type A strains associated with PAR and non-toxigenic capsular type A strains associated with pneumonia represent distinct subpopulations of P. multocida that can be differentiated by their OMP-types. The association of capsular types A and D with strains of the same OMP-types, and the absence and presence of the toxA gene in strains of the same OMP-types, suggest that horizontal transfer of capsular biosynthesis and toxA genes has occurred between strains representing certain subpopulations of P. multocida. INTRODUCTIONPasteurella multocida is the aetiological agent of progressive atrophic rhinitis (PAR) of swine and is of considerable economic importance to the pig-rearing industry throughout the world (Chanter & Rutter, 1989). PAR is characterized by atrophy of the nasal turbinate bones which, in severe cases, can lead to facial distortion. P. multocida strains associated with PAR usually produce a 145-kDa dermonecrotic toxin, which is encoded by the toxA gene (Lax et al., 1990;Buys et al., 1990). This toxin induces osteolysis in the turbinate bones and plays an important role in the pathogenesis of PAR (Kamp & Kimman, 1988). Toxigenic strains associated with PAR are most frequently of capsular type D (Eamens et al., 1988;Foged et al., 1988;Gardner et al., 1994;Lariviere et al., 1992), although toxigenic isolates of capsular type A can also be involved in the disease (Fussing et al., 1999;Sakano et al., 1992). Non-toxigenic P. multocida strains are not usually associated with PAR and confirmation of toxin production is important for the diagnosis and control of the disea...

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In contrast to isolates associated with PAR, P. multocida strains isolated from pneumonic lungs are usually non-toxigenic and of capsular type A (Pijoan et al, 1983(Pijoan et al, , 1984Rubies et al, 2002;Zhao et al, 1992). However, a small proportion of lung isolates are toxigenic and/or possess capsular type D (Pijoan et al, 1984;Rubies et al, 2002;Choi et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

Davies

MacCorquodale

Baillie

et al. 2003

Journal of Medical Microbiology

104

View full text Add to dashboard Cite

show abstract

Pasteurellosis

Brockmeier²

2019

Diseases of Swine

View full text Add to dashboard Cite

Genetic diversity and associated pathology of Pasteurella multocida isolated from porcine pneumonia

Pors

Hansen

Jensen

et al. 2011

Veterinary Microbiology

View full text Add to dashboard Cite

Pasteurella multocida is a widespread respiratory pathogen in pigs associated with atrophic rhinitis and contributing to aggravation of the pulmonary lesions. The aims of the present study were to characterize isolates of P. multocida from porcine bronchopneumonia by pulsed-field gel electrophoresis (PFGE), PCR based capsular typing and multilocus sequence typing (MLST) and to compare clonal complexes outlined with the type of histological lung lesions to investigate if a correlation between clonal lineages and lesions might exist. Isolates of P. multocida were obtained from cases of cranioventrally located porcine bronchopneumonia. All lung lesions were described and classified according to histological lesions. A total of 139 isolates, from lung (n=111), pericardial sac (n=21) and kidney (n=7) of 111 pigs were described using PFGE with ApaI as the restriction enzyme. Furthermore, 20 and 29 isolates were characterized by capsular serotyping and multilocus sequence typing, respectively. PFGE demonstrated 15 different clusters showing 50% or more similarity. All selected isolates were of capsular serotype A and only three main sequence types (ST) were detected among the isolates. Associations were not found between histopathology and clonal complexes of P. multocida. In conclusion, PFGE demonstrated a high diversity of genotypes of P. multocida associated with porcine bronchopneumonia. However, isolates obtained mainly belonged to few STs, indicating that isolates of P. multocida associated with porcine bronchopneumonia originates from a limited number of clonal lineages and therefore might have adapted to porcine hosts. No correlation was demonstrated between genotypes and types of lesions, and extra-pulmonary spreading was only rarely demonstrated.

show abstract

Plasmid and restriction endonuclease patterns in Pasteurella multocida isolated from a swine pyramid

Cited by 10 publications

References 19 publications

Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

Pasteurellosis

Genetic diversity and associated pathology of Pasteurella multocida isolated from porcine pneumonia

Contact Info

Product

Resources

About