Plasmid-controlled resistance to copper in Escherichia coli

Tetaz, T J; Luke, R. K. J.

doi:10.1128/jb.154.3.1263-1268.1983

Cited by 165 publications

(72 citation statements)

References 20 publications

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“…But in respect of Cu, the level of resistance was almost the same at the range of 20 mmol 1-' both for Acidiphilium strains and an Escherichia coli isolate (Tetaz and Luke 1983). It may therefore be suggested that Acidzphillum strains, in general, probably represent the highest metal-tolerant bacteria among the heterotrophs although their growth was inhibited at a very low concentration of Hg and Ag (Bhattacharyya et al 1991).…”

Section: Resultsmentioning

confidence: 95%

Extreme tolerance to cadmium and high resistance to copper, nickel and zinc in different Acidiphilium strains

Mahapatra

Banerjee

1996

Lett Appl Microbiol

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An Acidiphilium cryptum strain and two Acidiphilium symbioticum strains exhibited extremely high tolerance to CdSO4, growing at a concentration of 700 mmol l‐1 or more in MGY medium (g l‐1 composition: KCl, 0.1; MgSO4.7H2O, 0.25; (NH4)2SO4, 2.0; K2HPO4, 0.25; glucose, 1.0; yeast extract, 0.1) of pH 3. Fairly high concentrations of ZnSO4 (200 > 125 mmol l‐1), NiSO4 (50 > 20 mmol l‐1) and CuSO4 (25 > 15 mmol l‐1) were also tolerated by these strains. Acidiphilium multivorum grew in the presence of NiSO4 (400 > 350 mmol l‐1), ZnSO4 (50 > 40 mmol l‐1), CdSO4 (40 > 20 mmol l‐1) and CuSO4 (20 > 10 mmol l‐1) while an Acidiphilium strain GS18h tolerated ZnSO4 (100 > 60 mmol l‐1), NiSO4 (50 > 30 mmol l‐1), CuSO4 (30 > 15 mmol l‐1) and CdSO4 (20 > 10 mmol l‐1) in the same medium. Acidiphilium angustum, on the other hand, was found to be metal sensitive—growth inhibition being observed at concentrations of 10 mmol l‐1 ZnSO4, 10 mmol l‐1 CuSO4, 20 mmol l‐1 NiSO4 and 0.2 mmol l‐1 CdSO4 in MGY medium. Acidiphilium organovorum also exhibited tolerance to these metal sulphates in this medium at the levels of 70 > 60 mmol l‐1 for Zn, 20 > 10 mmol l‐1 for Cd and Ni, and 10 > 5 mmol l‐1 for Cu. This strain, interestingly enough, showed a much higher level of tolerance in MAGY medium (g l‐1 composition: KCl, 0.15; MgSO4.7H2O, 3.36; K2HPO4, 0.15; (NH4)2SO4, 0.15; Al2(SO4)3.18H2O, 2.25; CaCl2.2H2O, 0.97; MnSO4.H2O, 0.12; glucose, 1.0; yeast extract, 0.1) of pH 3 than in MGY medium. Replacement of carbon source, however, did not affect the tolerance levels to any great extent.

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Section: Resultsmentioning

confidence: 95%

Extreme tolerance to cadmium and high resistance to copper, nickel and zinc in different Acidiphilium strains

Mahapatra

Banerjee

1996

Lett Appl Microbiol

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“…An E. coli copper resistance plasmid, pRJ 1004, was found in a strain isolated from the effluent of a piggery [77] where copper sulfate, used as a food supplement, possibly acted as a selective agent. Inducible resistance to copper conferred by pRJl004 was found to be due to decreased metal uptake [78].…”

Section: "Fhe E Coli Systemmentioning

confidence: 99%

Copper resistance mechanisms in bacteria and fungi

Cervantes¹

1994

FEMS Microbiology Reviews

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Copper is both an essential micronutrient and a toxic heavy metal for most living cells. The presence of high concentrations of cupric ions in the environment promotes the selection of microorganisms possessing genetic determinants for copper resistance. Several examples of chromosomal and plasmid copper-resistance systems in bacteria have been reported, and the mechanisms of resistance have started to be understood at the molecular level. Bacterial mechanisms of copper resistance are related to reduced copper transport, enhanced efflux of cupric ions, or copper complexation by cell components. Copper tolerance in fungi has also been ascribed to diverse mechanisms involving trapping of the metal by cell-wall components, altered uptake of copper, extracellular chelation or precipitation by secreted metabolites, and intracellular complexing by metallothioneins and phytochelatins; only the metallothionein chelation mechanism has been approached with molecular detail.

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“…1989;Brown ef a/., 1994). The genetic approach involved characterization of a plasmid system that confers resistance to high copper levels (Tetaz and Luke. 1983;Reuch, 1986;Brown etai, 1994) and, inaddition, isolation of chromosomal mutations that result in 'hypersensitivity' of the Esdieridiia co//ceil to copper salts (Rouch, 1986;Rogers eta!..…”

Section: Introductionmentioning

confidence: 99%

Molecular genetics of a chromosomal locus involved in copper tolerance in Escherichia coli K‐12

Fong

Camakaris

Lee

1995

Molecular Microbiology

107

125

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The cutA locus, presumably involved in copper tolerance in Escherichia coli, was characterized by a mutation leading to copper sensitivity. Copper-accumulation measurements with radioactive 64Cu2+ showed increased uptake by cutA copper-sensitive mutant cells, and reduced uptake when the cutA mutation was complemented in trans. The locus was mapped using complementation of the cutA mutant to partial copper tolerance with wild-type chromosomal fragments. The 3.2 kb DNA region involved in cutA was sequenced and analysed, revealing three significant open reading frames, none of which had been previously published. The products of all three open reading frames were identified, when synthesized with the T7 phage promoter expression system, as polypeptides of about 50 kDa, 24 kDa, and 13 kDa, consistent with the sizes predicted from the DNA sequences. The 50 kDa and 24 kDa polypeptides were found in the bacterial inner membrane, and the 13 kDa polypeptide with the cytoplasmic fraction. In addition to being required for copper tolerance, cutA affects tolerance levels to zinc, nickel, cobalt and cadmium salts. Transcriptional fusions of cutA with the lux operon showed induction by copper, zinc, nickel, cobalt and, to a lesser extent, cadmium, manganese and silver salts.

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Plasmid-controlled resistance to copper in Escherichia coli

Cited by 165 publications

References 20 publications

Extreme tolerance to cadmium and high resistance to copper, nickel and zinc in different Acidiphilium strains

Extreme tolerance to cadmium and high resistance to copper, nickel and zinc in different Acidiphilium strains

Copper resistance mechanisms in bacteria and fungi

Molecular genetics of a chromosomal locus involved in copper tolerance in Escherichia coli K‐12

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