Plasmid profile typing provides a method for the differentiation of strains of Salmonella enteritidis phage type 4 isolated in Turkey

Erdem, Birsel; Threlfall, E. J.; Schofield, S. L.; Ward, L. R.; Rowe, B.

doi:10.1111/j.1472-765x.1994.tb00960.x

Cited by 13 publications

(6 citation statements)

References 10 publications

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“…The plasmid profiles of our isolates showed similarities with their report. Also several researchers reported that 55 kb plasmid, the plasmid that we identified in RTE-1, is the most dominating plasmid among Salmonella strains, especially in food-originated isolates ( Erdem et al , 1994 ; Stubbs et al , 1994 ).…”

Section: Resultsmentioning

confidence: 76%

Characterization of antibiotic resistance in Salmonella enterica isolates determined from ready-to-eat (RTE) salad vegetables

Taban¹,

Aytaç²,

Akkoç³

et al. 2013

Braz. J. Microbiol.

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In the last decade, ready-to-eat (RTE) salad vegetables are gaining increasing importance in human diet. However, since they are consumed fresh, inadequate washing during processing can bring on some foodborne illnesses, like salmonellosis, since these food items have natural contamination from soil and water. During 2009–2010, a total of 81 samples were purchased arbitrarily from local markets in Ankara, and were examined for Salmonella contamination. Salmonella screening was performed by using anti-Salmonella magnetic beads system and polymerase chain reaction (PCR) identification of the suspected colonies. Then, the antibiotic resistance profiles of four Salmonella strains identified (strains RTE-1, RTE-2, RTE-3, and RTE-4) were also investigated, since the mechanism by which Salmonella spp. have accumulated antibiotic resistance genes is of interest. All strains showed resistance against sulfonamides (MIC > 128 mg/L). Further results suggested that associated sulfonamide resistance genes were encoded by the 55.0 kb plasmid of strain RTE-1 that involves no integrons. As a result of using two primers (P1254 and P1283) in randomly amplified polymorphic DNA-PCR (RAPD-PCR) analysis, two common amplicons (364 bp and 1065 bp) were determined. The findings of this study provide support to the adoption of guidelines for the prudent use of antibiotics in order to reduce the number of pathogens present on vegetable and fruit farms. Besides, since it is shown that these bacteria started to gain resistance to antibiotics, it is necessary to further investigate the prevalence of them in foods.

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Section: Resultsmentioning

confidence: 76%

Characterization of antibiotic resistance in Salmonella enterica isolates determined from ready-to-eat (RTE) salad vegetables

Taban¹,

Aytaç²,

Akkoç³

et al. 2013

Braz. J. Microbiol.

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“…are usually defined phenotypically by serotyping and antimicrobial resistance tests in microbiology laboratories of Iran. Since phenotyping data suggest the clonal diffusion of a limited number of local strains, there is a need for practical techniques to discriminate Salmonella isolates, and to evaluate the association of Salmonella strains in epidemiological studies [35]. Here, we presented the results of a molecular study of extended-spectrum cephalosporins resistant Salmonella strains isolated from pediatric patients in Iran.…”

Section: Discussionmentioning

confidence: 99%

The Study of Genetic Relationship Among Third Generation Cephalosporin-resistant Salmonella enterica Strains by ERIC-PCR

Ranjbar¹,

Naghoni²,

Yousefi³

et al. 2013

TOMICROJ

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Background and Objectives: Salmonella is an important food-borne pathogen responsible for disease in humans and animals. The aim of this study was to investigate the genetic relationship among third generation cephalosporin-resistant Salmonella enterica strains by Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR. Methods: The study included all Salmonella isolates obtained from clinical cases in a pediatric hospital in Tehran, Iran during 2006 to 2009. Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute. The genetic relationship between third generation cephalosporins-resistant Salmonella enterica strains was determined using ERIC-PCR. Results: Of 136 Salmonella enterica isolates recovered from pediatric patients, six isolates including four Salmonella enterica serotype Infantis and two Salmonella enterica serotype Enteritidis showed an extended-spectrum cephalosporins resistant phenotype. ERIC-PCR differentiated Salmonella enterica serotypes Infantis and Enteritidis into 2 distinct clusters arbitrarily named as E1 and E2. Profile E1 was found in two Salmonella enterica serotype Enteritidis isolates, and profile E2 was found in four Salmonella enterica serotype Infantis isolates. Conclusion: Extended-spectrum cephalosporins resistant Salmonella could be attributed to a few predominant serotypes including Enteritidis and Infantis in this study. Genetic analysis using ERIC-PCR showed that closely related clones are responsible for the occurrence of extended-spectrum cephalosporins resistant Salmonella infection in Tehran.

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“…In 1994, it was reported that, among the S. enteritidis strains isolated in Ankara, the predominant phage type was PT 4 [36].…”

Section: Discussionmentioning

confidence: 99%

Phage types, antibiotic susceptibilities and plasmid profiles of Salmonella typhimurium and Salmonella enteritidis strains isolated in Istanbul, Turkey

Anğ-Küçüker

Tolün

Helmuth

et al. 2000

Clinical Microbiology and Infection

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Plasmid profile typing provides a method for the differentiation of strains of Salmonella enteritidis phage type 4 isolated in Turkey

Cited by 13 publications

References 10 publications

Characterization of antibiotic resistance in Salmonella enterica isolates determined from ready-to-eat (RTE) salad vegetables

Characterization of antibiotic resistance in Salmonella enterica isolates determined from ready-to-eat (RTE) salad vegetables

The Study of Genetic Relationship Among Third Generation Cephalosporin-resistant Salmonella enterica Strains by ERIC-PCR

Phage types, antibiotic susceptibilities and plasmid profiles of Salmonella typhimurium and Salmonella enteritidis strains isolated in Istanbul, Turkey

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