Plasmid transfers by conjugation and electroporation in Pediococcus acidilactici

Abstract

W.J. KIM, B. RAY AND M.C. JOHNSON. 1992. Plasmid profiles of wild and mutant strains of Pediococcus acidilactici M showed that a 53.7 kb plasmid (pPR72) encodes the sucrose hydrolysis trait (Suc+) and an 11.1 kb plasmid encodes the bacteriocin production trait (Pap+). Neither of these plasmids encode traits involving fermentation of other carbohydrates, antibiotic resistance or resistance to bacteriocin. Broad host‐range plasmids (pAMβ1 and pIP501) from Enterococcus faecalis and plasmid pPR72 from Ped. acidilactici were conjugally transferred by filter mating into two strains of Ped. acidilactici. Four plasmids, ranging in size from 4.4 to 53.7 kb, were also transferred into Ped. acidilactici strains by electroporation. Optimum transformation of the 4.4 kb plasmid, pGK12, was obtained at a DNA concentration of 1 μg/220 μl. The same amount of DNA gave lower transformation frequencies as the plasmid size increased. Results of these studies indicated that both conjugation and electroporation can be used to transfer plasmid‐linked traits in Ped. acidilactici strains.

Anti-listeria activity of Pediococcus pentosaceus BCC 3772 and application as starter culture for Nham, a traditional fermented pork sausage

“…property is in agreement with previously reported results (Bhunia, Johnson, & Kalchayanand, 1991;Gaussier, Morency, Lavoie, & Subirade, 2003;Héchard, Dérjard, Letellier, & Cenatiempo, 1992;Jack, Tagg, & Ray, 1995;Kim, Ray, & Johnson, 1992;Le Blay, Lacroix, Zihler, & Fliss, 2007).…”

Characterization and determination of the factors affecting anti-listerial bacteriocins from Lactobacillus plantarum and Pediococcus pentosaceus isolated from dairy milk products

“…However, strains of Pediococcus acidilactici and Pediococcus pentosaceus have been reported to produce bacteriocins (Gonzales and Kunka, 1987;Biswas et al, 1991;Kim et al, 1992;Elegado et al, 1997;Gurira and Buys, 2005).…”

“…Some of these are designated as pediocin AcH by P. acidilactici H, E, F, and M (Bhunia et al, 1987;Ray et al, 1989;Kim et al, 1992), pediocin PA-1 by P. acidilactici PAC 1.0 (Gonzales and Kunka, 1987), pediocin JD by P. acidilactici SJ-1 (Schved et al, 1993), pediocin 5 by P. acidilactici UL5 (Huang et al, 1996), pediocin A by P. pentosaceus FBB-61 (Etchells et al, 1964;Flemming et al, 1975), pediocin N5p by P. pentosaceus (Strasser de Saad et al, 1995), and pediocin ST18 by P. pentosaceus (Todorov and Dicks, 2005).…”

Discovering lactic acid bacteria by genomics

“…Plasmids can be conjugally transferred between Pediococcus and Enterococcus, Streptococcus, or Lactococcus (Gonzalez & Kunka 1983). Electroporation has been utilized to introduce plasmids into pediococci, including P. pentosaceus (Kim et al 1992;Caldwell 1996).…”