“Plasmo2D”:  An Ancillary Proteomic Tool to Aid Identification of Proteins from Plasmodium falciparum

Khachane, Amit N.; Kumar, Ranjit; Jain, Suresh P.; Jain, Shubham; Gowrishankar, Banumathy; Singh, Varsha; Nagpal, Saurabh; Tatu, Utpal

doi:10.1021/pr050289p

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…In the mature red blood cell, P. falciparum has three stages of development: the ring stage, the trophozoite stage and the schizont stage. PfHSP90 is known to be expressed in all three stages of the parasite life cycle [17]. Inhibition of PfHSP90 function using GA arrests parasite development from the ring stage to the trophozoite stage during the intra-erythrocytic cycle, suggesting that PfHSP90 may play a critical role in this process [16,18].…”

Section: Plasmodium Hsp90mentioning

confidence: 99%

Protozoan HSP90-Heterocomplex: Molecular Interaction Network and Biological Significance

Figueras¹,

Echeverria²,

Angel

2014

CPPS

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The HSP90 chaperone is a highly conserved protein from bacteria to higher eukaryotes. In eukaryotes, this chaperone participates in different large complexes, such as the HSP90 heterocomplex, which has important biological roles in cell homeostasis and differentiation. The HSP90-heterocomplex is also named the HSP90/HSP70 cycle because different co-chaperones (HIP, HSP40, HOP, p23, AHA1, immunophilins, PP5) participate in this complex by assembling sequentially, from the early to the mature complex. In this review, we analyze the conservation and relevance of HSP90 and the HSP90-heterocomplex in several protozoan parasites, with emphasis in Plasmodium spp., Toxoplasma spp., Leishmania spp. and Trypanosoma spp. In the last years, there has been an outburst of studies based on yeast two-hybrid methodology, co-immunoprecipitation-mass spectrometry and bioinformatics, which have generated a most comprehensive protein-protein interaction (PPI) network of HSP90 and its co-chaperones. This review analyzes the existing PPI networks of HSP90 and its co-chaperones of some protozoan parasites and discusses the usefulness of these powerful tools to analyze the biological role of the HSP90-heterocomplex in these parasites. The generation of a T. gondii HSP90 heterocomplex PPI network based on experimental data and a recent Plasmodium HSP90 heterocomplex PPI network are also included and discussed. As an example, the putative implication of nuclear transport and chromatin (histones and Sir2) as HSP90-heterocomplex interactors is here discussed.

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Section: Plasmodium Hsp90mentioning

confidence: 99%

Protozoan HSP90-Heterocomplex: Molecular Interaction Network and Biological Significance

Figueras¹,

Echeverria²,

Angel

2014

CPPS

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“…PlasmoDB additionally has links to proteome data and also allows access to the interactome data. Proteome experiments based on 2D-gel electrophoresis can furthermore be aided by Plasmo2D software to allow the identification of proteins in such platforms [102]. …”

Section: Introductionmentioning

confidence: 99%

Integration and mining of malaria molecular, functional and pharmacological data: how far are we from a chemogenomic knowledge space?

Birkholtz

Bastien

Wells

et al. 2006

Malar J

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The organization and mining of malaria genomic and post-genomic data is important to significantly increase the knowledge of the biology of its causative agents, and is motivated, on a longer term, by the necessity to predict and characterize new biological targets and new drugs. Biological targets are sought in a biological space designed from the genomic data from Plasmodium falciparum, but using also the millions of genomic data from other species. Drug candidates are sought in a chemical space containing the millions of small molecules stored in public and private chemolibraries. Data management should, therefore, be as reliable and versatile as possible. In this context, five aspects of the organization and mining of malaria genomic and post-genomic data were examined: 1) the comparison of protein sequences including compositionally atypical malaria sequences, 2) the high throughput reconstruction of molecular phylogenies, 3) the representation of biological processes, particularly metabolic pathways, 4) the versatile methods to integrate genomic data, biological representations and functional profiling obtained from Xomic experiments after drug treatments and 5) the determination and prediction of protein structures and their molecular docking with drug candidate structures. Recent progress towards a grid-enabled chemogenomic knowledge space is discussed.

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Antimalarials

Smithson¹,

Guiguemde²,

Guy³

2010

Burger's Medicinal Chemistry and Drug Discovery

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While the treatment of malaria has until recently relied upon a small number of therapeutic agents with a few mechanisms of action, the past decade has seen a huge growth in the number of targets being addressed and new agents being pushed to the clinic. This chapter briefly reviews existing agents and close homologs in development and then carefully explores new targets and new chemotypes being developed.

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“Plasmo2D”: An Ancillary Proteomic Tool to Aid Identification of Proteins from Plasmodium falciparum

Cited by 7 publications

References 22 publications

Protozoan HSP90-Heterocomplex: Molecular Interaction Network and Biological Significance

Protozoan HSP90-Heterocomplex: Molecular Interaction Network and Biological Significance

Integration and mining of malaria molecular, functional and pharmacological data: how far are we from a chemogenomic knowledge space?

Antimalarials

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