Plasmodium vivax binds host CD98hc (SLC3A2) to enter immature red blood cells

Malleret, Benoît; Sahili, Abbas El; Tay, Matthew Zirui; Carissimo, Guillaume; Ong, Alice Soh Meoy; Novera, Wisna; Lin, Jianqing; Suwanarusk, Rossarin; Kosaisavee, Varakorn; Chu, Trang T. T.; Sinha, Ameya; Howland, Shanshan W.; Yiping, Fan; Gruszczyk, Jakub; Tham, Wai‐Hong; Colin, Yves; Maurer‐Stroh, Sebastian; Snounou, Georges; Ng, Lisa F. P.; Chan, Jerry Kok Yen; Chacko, Ann‐Marie; Lescar, Julien; Chandramohanadas, Rajesh; Nosten, François; Russell, Bruce; Rénia, Laurent

doi:10.1038/s41564-021-00939-3

Cited by 30 publications

(33 citation statements)

References 46 publications

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“…The 11 Pv-RBPs contain five full-length genes, three partial genes and three pseudogenes [128]. PvRBP2a has been shown to bind CD98 on reticulocytes with high affinity [129]. Cryo-electron microscopy (cryo-EM), immunoprecipitation, and fluorescence resonance energy transfer (FRET)-based assays further confirmed the specific binding of PvRBP2b to CD71 [130,131].…”

Section: Role Of Plasmodium Ligands In Erythrocytic Invasionmentioning

confidence: 87%

Zoonotic Malaria: Non-Laverania Plasmodium Biology and Invasion Mechanisms

et al. 2021

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Malaria, which is caused by Plasmodium parasites through Anopheles mosquito transmission, remains one of the most life-threatening diseases affecting hundreds of millions of people worldwide every year. Plasmodium vivax, which accounts for the majority of cases of recurring malaria caused by the Plasmodium (non-Laverania) subgenus, is an ancient and continuing zoonosis originating from monkey hosts probably outside Africa. The emergence of other zoonotic malarias (P. knowlesi, P. cynomolgi, and P. simium) further highlights the seriousness of the disease. The severity of this epidemic disease is dependent on many factors, including the parasite characteristics, host-parasite interactions, and the pathology of the infection. Successful infection depends on the ability of the parasite to invade the host; however, little is known about the parasite invasion biology and mechanisms. The lack of this information adds to the challenges to malaria control and elimination, hence enhancing the potential for continuation of this zoonosis. Here, we review the literature describing the characteristics, distribution, and genome details of the parasites, as well as host specificity, host-parasite interactions, and parasite pathology. This information will provide the basis of a greater understanding of the epidemiology and pathogenesis of malaria to support future development of strategies for the control and prevention of this zoonotic infection.

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Section: Role Of Plasmodium Ligands In Erythrocytic Invasionmentioning

confidence: 87%

Zoonotic Malaria: Non-Laverania Plasmodium Biology and Invasion Mechanisms

et al. 2021

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“…Considering that BIAbs activity is associated with strain-transcending inhibitory immune responses, we can assume that the detection of anti-DBPII antibodies against a range of diverse allelic DBPII variants seems to be more relevant than levels of the antibody per se in our multiplex assay. In this scenario, it would be interesting to explore whether the inclusion of additional antigens involved in the reticulocyte invasion would increase the chance of detecting BIAbs; for example, the P. vivax reticulocyte-binding protein (PvRBPs) family that are involved in novel reticulocyte invasion pathways (52)(53)(54). Also, a distinct erythrocyte-binding protein FIGURE 4 | Association between DBPII antibody response as detected in the DBPII-multiplexed microsphere-based cytometric assay and BIAbs as determined by standard COS-7 cytoadherence assay.…”

Section: Discussionmentioning

confidence: 99%

Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders

et al. 2021

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Malaria remains a major public health problem worldwide, and Plasmodium vivax is the most widely distributed malaria parasite. Naturally acquired binding inhibitory antibodies (BIAbs) to region II of the Duffy binding protein (DBPII), a P. vivax ligand that is critical for reticulocyte invasion, are associated with a reduced risk of clinical malaria. Owing to methodological issues in evaluating antibodies that inhibit the DBPII–DARC interaction, a limited number of studies have investigated DBPII BIAbs in P. vivax-exposed populations. Based on the assumption that individuals with a consistent BIAb response are characterized by strain-transcending immune responses, we hypothesized that detecting broadly reactive DBPII antibodies would indicate the presence of BIAb response. By taking advantage of an engineered DBPII immunogen targeting conserved DBPII neutralizing epitopes (DEKnull-2), we standardized a multiplex flow cytometry-based serological assay to detect broadly neutralizing IgG antibodies. For this study, a standard in vitro cytoadherence assay with COS-7 cells expressing DBPII was used to test for DBPII BIAb response in long-term P. vivax-exposed Amazonian individuals. Taken together, the results demonstrate that this DBPII-based multiplex assay facilitates identifying DBPII BIAb carriers. Of relevance, the ability of the multiplex assay to identify BIAb responders was highly accurate when the positivity for all antigens was considered. In conclusion, the standardized DBPII-based flow cytometric assay confirmed that DBPII-BIAb activity was associated with the breadth rather than the magnitude of anti-DBPII antibodies. Altogether, our results suggest that multiplex detection of broadly DBPII-reactive antibodies facilitates preliminary screening of BIAb responders.

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“…These include the PvDBP and the Reticulocyte Binding Protein (PvRBP) family members that have been shown to adhere to reticulocytes [ 300 , 316 – 320 ]. Specifically, young CD71+ and CD98+ reticulocytes were identified as the host cells for P. vivax [ 13 , 318 , 321 , 322 ] (reviewed in [ 323 ]). Importantly, the PvRBPs are related to the subsequently discovered P. falciparum Reticulocyte Binding Protein homologues (Rh) [ 324 – 326 ] and the Rh5 family member [ 327 ] has progressed from pre-clinical trials in A. nancymaae monkeys to clinical vaccine trials in humans [ 328 – 331 ].…”

Section: Twenty-first Century—turning Point In Malaria Researchmentioning

confidence: 99%

Systems biology of malaria explored with nonhuman primates

Galinski

2022

Malar J

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Abstract“The Primate Malarias” book has been a uniquely important resource for multiple generations of scientists, since its debut in 1971, and remains pertinent to the present day. Indeed, nonhuman primates (NHPs) have been instrumental for major breakthroughs in basic and pre-clinical research on malaria for over 50 years. Research involving NHPs have provided critical insights and data that have been essential for malaria research on many parasite species, drugs, vaccines, pathogenesis, and transmission, leading to improved clinical care and advancing research goals for malaria control, elimination, and eradication. Whilst most malaria scientists over the decades have been studying Plasmodium falciparum, with NHP infections, in clinical studies with humans, or using in vitro culture or rodent model systems, others have been dedicated to advancing research on Plasmodium vivax, as well as on phylogenetically related simian species, including Plasmodium cynomolgi, Plasmodium coatneyi, and Plasmodium knowlesi. In-depth study of these four phylogenetically related species over the years has spawned the design of NHP longitudinal infection strategies for gathering information about ongoing infections, which can be related to human infections. These Plasmodium-NHP infection model systems are reviewed here, with emphasis on modern systems biological approaches to studying longitudinal infections, pathogenesis, immunity, and vaccines. Recent discoveries capitalizing on NHP longitudinal infections include an advanced understanding of chronic infections, relapses, anaemia, and immune memory. With quickly emerging new technological advances, more in-depth research and mechanistic discoveries can be anticipated on these and additional critical topics, including hypnozoite biology, antigenic variation, gametocyte transmission, bone marrow dysfunction, and loss of uninfected RBCs. New strategies and insights published by the Malaria Host–Pathogen Interaction Center (MaHPIC) are recapped here along with a vision that stresses the importance of educating future experts well trained in utilizing NHP infection model systems for the pursuit of innovative, effective interventions against malaria.

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Plasmodium vivax binds host CD98hc (SLC3A2) to enter immature red blood cells

Cited by 30 publications

References 46 publications

Zoonotic Malaria: Non-Laverania Plasmodium Biology and Invasion Mechanisms

Zoonotic Malaria: Non-Laverania Plasmodium Biology and Invasion Mechanisms

Multiplexed Microsphere-Based Flow Cytometric Assay to Assess Strain Transcending Antibodies to Plasmodium vivax Duffy Binding Protein II Reveals an Efficient Tool to Identify Binding-Inhibitory Antibody Responders

Systems biology of malaria explored with nonhuman primates

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