Plasmodium yoelii:Peptide Immunization Induces Protective CD4+T Cells against a Previously Unrecognized Cryptic Epitope of the Circumsporozoite Protein

Takita-Sonoda, Yoshiko; Tsuji, Moriya; Kamboj, Kamal; Nussenzweig, Ruth S.; Clavijo, Pedro; Zavala, Fidel

doi:10.1006/expr.1996.0108

Cited by 28 publications

(18 citation statements)

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“…To our knowledge, this represents the first study in which the exact molecular nature of a Plasmodium-derived, subdominant CTL epitope has been defined. Previous reports have described cryptic T-cell epitopes in the CSP of Plasmodium falciparum (11), Plasmodium berghei (16), and P. yoelii (34).…”

Section: Discussionmentioning

confidence: 97%

A Subdominant CD8⁺Cytotoxic T Lymphocyte (CTL) Epitope from thePlasmodium yoeliiCircumsporozoite Protein Induces CTLs That Eliminate Infected Hepatocytes from Culture

Franke

Sette²,

Sacci

et al. 2000

Infect Immun

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Section: Discussionmentioning

confidence: 97%

A Subdominant CD8⁺Cytotoxic T Lymphocyte (CTL) Epitope from thePlasmodium yoeliiCircumsporozoite Protein Induces CTLs That Eliminate Infected Hepatocytes from Culture

Franke

Sette²,

Sacci

et al. 2000

Infect Immun

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“…(25,53,64), and Plasmodium spp. (18,22,46,60) have all been identified as previously known conserved surface, abundant, or secreted proteins. In contrast, the cDNA expression library provides an unbiased and immunologically relevant approach that can be applied to identify any CD4 T cell-stimulating antigen.…”

Section: Discussionmentioning

confidence: 99%

The Toxoplasma gondii Peptide AS15 Elicits CD4 T Cells That Can Control Parasite Burden

et al. 2012

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ABSTRACTThe apicomplexan parasiteToxoplasma gondiican cause severe disease in immunocompromised individuals. Previous studies in mice have focused largely on CD8+T cells, and the role of CD4 T cells is relatively unexplored. Here, we show that immunization of the C57BL/6 strain of mice, in which the immunodominant CD8 T cell response to the parasite dense-granule protein GRA6 cannot be generated, leads to a prominent CD4 T cell response. To identify the CD4 T cell-stimulating antigens, we generated aT. gondii-specific,lacZ-inducible, CD4 T cell hybridoma and used it as a probe to screen aT. gondiicDNA library. We isolated a cDNA encoding a protein of unknown function that we call CD4Ag28m and identified the minimal peptide, AS15, which was presented by major histocompatibility complex (MHC) class II molecules to the CD4 T cells. Immunization of mice with the AS15 peptide provided significant protection against subsequent parasite challenge, resulting in a lower parasite burden in the brain. Our findings identify the first CD4 T cell-stimulating peptide that can confer protection against toxoplasmosis and provide an important tool for the study of CD4 T cell responses and the design of effective vaccines against the parasite.

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“…The relative number of CS-specific, IFN-␥-secreting, CD8 ϩ and CD4 ϩ T cells, in the spleens of mice immunized by using the different regimens, was determined by direct ex-vivo ELISPOT assays, as previously described (13,14). For these assays, we used MHC-compatible A20.2J target cells coated with the peptides SYVPSAEQI (10) or YNRNIVNRLLGDALNGKPEEK (15), which contain the CS-derived H-2K d -restricted epitopes recognized by CS-specific CD8 ϩ or CD4 ϩ T cells, respectively.…”

Section: Methodsmentioning

confidence: 99%

Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen

Bruña–Romero

González‐Aseguinolaza

Hafalla

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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111

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We report that complete protection against malaria and total inhibition of liver stage development and parasitemia was obtained in 100% of BALB͞c mice primed with a replication-defective recombinant adenovirus expressing the circumsporozoite (CS) protein of Plasmodium yoelii (AdPyCS), followed by a booster with an attenuated recombinant vaccinia virus, expressing the same malaria antigen, VacPyCS. We found increased levels of activated CS-specific CD8 ؉ and CD4 ؉ T cells, higher anti-sporozoite antibody titers, and greater protection in these mice, when the time between priming and boosting with these two viral vectors was extended from 2 to 8 or more weeks. Most importantly, by using this immunization regimen, the protection of the immunized mice was found to be long-lasting, namely complete resistance to infection of all animals 3 1͞2 months after priming. These results indicate that immunization with AdPyCS generates highly effective memory T and B cells that can be recalled long after priming by boosting with VacPyCS.

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Plasmodium yoelii:Peptide Immunization Induces Protective CD4+T Cells against a Previously Unrecognized Cryptic Epitope of the Circumsporozoite Protein

Cited by 28 publications

References 0 publications

A Subdominant CD8⁺Cytotoxic T Lymphocyte (CTL) Epitope from thePlasmodium yoeliiCircumsporozoite Protein Induces CTLs That Eliminate Infected Hepatocytes from Culture

A Subdominant CD8⁺Cytotoxic T Lymphocyte (CTL) Epitope from thePlasmodium yoeliiCircumsporozoite Protein Induces CTLs That Eliminate Infected Hepatocytes from Culture

The Toxoplasma gondii Peptide AS15 Elicits CD4 T Cells That Can Control Parasite Burden

Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen

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Plasmodium yoelii:Peptide Immunization Induces Protective CD4+T Cells against a Previously Unrecognized Cryptic Epitope of the Circumsporozoite Protein

Cited by 28 publications

References 0 publications

A Subdominant CD8+Cytotoxic T Lymphocyte (CTL) Epitope from thePlasmodium yoeliiCircumsporozoite Protein Induces CTLs That Eliminate Infected Hepatocytes from Culture

A Subdominant CD8+Cytotoxic T Lymphocyte (CTL) Epitope from thePlasmodium yoeliiCircumsporozoite Protein Induces CTLs That Eliminate Infected Hepatocytes from Culture

The Toxoplasma gondii Peptide AS15 Elicits CD4 T Cells That Can Control Parasite Burden

Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen

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A Subdominant CD8⁺Cytotoxic T Lymphocyte (CTL) Epitope from thePlasmodium yoeliiCircumsporozoite Protein Induces CTLs That Eliminate Infected Hepatocytes from Culture

A Subdominant CD8⁺Cytotoxic T Lymphocyte (CTL) Epitope from thePlasmodium yoeliiCircumsporozoite Protein Induces CTLs That Eliminate Infected Hepatocytes from Culture