Plasmonic Cell‐Derived Nanocomposites for Light‐Controlled Cargo Release inside Living Cells

Abstract

Translating the potential of thermoplasmonics to cell‐derived nanomaterials offers exciting opportunities to fabricate beyond state‐of‐art artificial biomimetic nanocomposites that upon illumination perform active tasks such as delivery of cargo in complex, dynamic media such as the cytosol of cells. Cell‐derived nanoparticles have shown stunning potential to implement cell‐specific functions, such as long blood circulation or targeting capabilities, into advanced drug delivery nanosystems. The biomimicry nanotechnology has now advanced to offer new and exciting opportunities to improve the commonly poor in vivo performance of most current nanomedicines, including evading the immune system and targeting specific tissues such as tumors, the latest remaining among the most wanted breakthroughs in nanomedicine. However, the use of cell‐derived nanocomposites as stimulus‐controlled drug delivery agents remains virtually unexplored. This study reports the fabrication of a plasmonic cell‐derived nanocomposite by integrating near‐infrared active gold nanorods in its structure. As a proof of concept, the plasmonic nanomembranes are loaded with cell non‐permeant antibodies, which upon near‐infrared stimulation can be released from the plasmonic nanomembranes into the cytosol of living cells, without impairing cell viability or the antibodies' function. These results set the stage for the development of photoactive cell‐derived nanocarriers, which in addition to cell‐specific functions promise straightforward access to spatiotemporal‐controlled intracellular delivery of antibodies.

Irradiation pretreatment enhances the therapeutic efficacy of platelet-membrane-camouflaged antitumor nanoparticles

“…Nevertheless, provided that the carrier is not endowed with antiphagocytic ability against the mononuclear phagocyte system in vivo, the bioavailability of ICG is still limited [7]. Cell membrane-based nanoparticles (CMBNPs) represent promising materials to overcome this shortcoming [8], as the functional molecules on the membrane, such as CD47 [9], CD45, and glycans, can send a "don't eat me" signal to the immune system [10]. The biomimetic strategy is plausibly beneficial for ICG delivery.…”

Modulation of Abundance and Location of High-Mobility Group Box 1 in Human Microglia and Macrophages under Oxygen–Glucose Deprivation

“…Biomimetic platelet cell-derived nanoparticles (cellsomes, CSM) were prepared following the protocol we previously described to obtain monodisperse cellsomes of size around 200 nm. , To create the CSMs, human platelet concentrate (#SER-PCEX) was purchased from Zen-bio©. Platelets were aliquoted at 5 mL (10 9 –10 11 cells) and stored at 4 °C until use.…”

Nanosized metal–organic frameworks as unique platforms for bioapplications

“…This term refers to the ability of self-recognition of cancer cells for its cell lineage or the evasion of the immune system. 82,83 Therefore, this strategy offers great advantages for its use in areas such as cancer therapy or vaccination.…”