Plasmonic colorimetric immunosensor based on Poly-HRP and AuNS etching for tri-modal readout of small molecule

Lu, Wenying; Tian, Yuan; Teng, W.D.; Qiu, Xuchun; Li, Ming

doi:10.1016/j.talanta.2023.124883

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…Generally, the amplification of a detection signal is an effective strategy to improve the sensitivity of immunoassays. The detection signal can be amplified using the biotin–streptavidin system, poly-enzyme conjugate, and novel nanoparticle probes [ 39 , 40 , 41 ]. Due to the high affinity between biotin and streptavidin, the biotin–streptavidin system has been widely used in immunoassays for sensitivity improvement.…”

Section: Introductionmentioning

confidence: 99%

“…Sun et al [ 39 ] developed a biotinylated nanobody and streptavidin–HRP-based ELISA for the detection of ochratoxin A in cereal, and the sensitivity of the method was improved 10 times compared with the traditional ELISA. Lu et al [ 40 ] developed a plasmonic colorimetric immunosensor for zearalenone detection using poly-HRP amplification, exhibiting an LOD of 0.04 ng/mL. The introduction of poly-HRP increased the amount of enzyme and generated more catalytic capacity.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Phage-Displayed Nanobody-Based Competitive Immunoassay for the Sensitive Detection of Soybean Agglutinin

Zhang,

Qiu,

You

et al. 2024

Foods

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Soybean agglutinin (SBA) is a primary antinutritional factor in soybeans that can inhibit the growth of humans and mammals, disrupt the intestinal environment, and cause pathological changes. Therefore, detecting and monitoring SBA in foods is essential for safeguarding human health. In this paper, M13 phage-displayed nanobodies against SBA were isolated from a naive nanobody library. An M13 phage-displayed nanobody-based competitive enzyme-linked immunosorbent assay (P-cELISA) was then established for SBA analysis using biotinylated anti-M13 phage antibody (biotin-anti-M13) and streptavidin poly-HRP conjugate (SA-poly-HRP). The biotin-anti-M13@SA-poly-HRP probe can easily amplify the detection signal without the chemical modifications of phage-displayed nanobodies. The established P-cELISA presented a linear detection range of 0.56–250.23 ng/mL and a limit of detection (LOD) of 0.20 ng/mL, which was 12.6-fold more sensitive than the traditional phage-ELISA. Moreover, the developed method showed good specificity for SBA and acceptable recoveries (78.21–121.11%) in spiked wheat flour, albumen powder, and whole milk powder. This study proposes that P-cELISA based on biotin-anti-M13@SA-poly-HRP may provide a convenient and effective strategy for the sensitive detection of SBA.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%