Plasmonic Nanoparticle Conjugation for Nucleic Acid Biosensing

Tadimety, Amogha; Zhang, Yichen; Molinski, John; Palinski, Timothy J.; Tsongalis, Gregory J.; Zhang, John X. J.

doi:10.1007/978-1-0716-1803-5_5

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…Second, a shorter DNA fragment/probe is used to recognize the target sequence. This increases the number of fragmented DNA sequences suitable for recognition [8]. Currently, there are no ready-made solutions that would fully meet the needs of the clinical diagnostics market.…”

Section: Introductionmentioning

confidence: 99%

High sensitivity electrochemical DNA sensors for detection of somatic mutations in FFPE samples

Egorova¹,

Grushevskaya²,

Крылова³

et al. 2022

Preprint

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We offer new high-performance label-free electrochemical impedimetric DNA sensors of nonfaradaic type. The DNA sensors based on a platform of crystalline carbon nanotube (CNT) arrays are fabricated by the Langmuir-Blodgett (LB) deposition technique. The CNT arrays are suspended on a nanoporous anodic alumina (aluminium oxide, AOA) support. Single-stranded (ss) 19-and 20-base oligonucleotides and double-stranded (ds) nucleotide sequences were used as probes for chosen molecular target -human KRAS (Kirsten Rat Sarcoma viral oncogene homolog) gene. Genomic deoxyribonucleic acids (DNAs) were isolated from placenta of healthy donors and FFPE (formalin-fixed, paraffinembedded) samples of tumor tissue. Raman spectral analysis and electrochemical dielectric spectroscopy were used to sequence the target DNAs. The optical and electrochemical detection (variants of DNA sequencing) were based on a screening effect that grows after the DNA homoduplex formation. We demonstrated that such technologies are very sensitive and allow to detect attomolar DNA concentrations and less. As result, the KRAS exon 2, codon 12, c.35G>A mutation was successfully discriminated in human genomic DNA isolated from FFPE colorectal cancer tumor tissue samples.

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Section: Introductionmentioning

confidence: 99%

High sensitivity electrochemical DNA sensors for detection of somatic mutations in FFPE samples

Egorova¹,

Grushevskaya²,

Крылова³

et al. 2022

Preprint

View full text Add to dashboard Cite

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Nanobiosensors based on on‐site detection approaches for rapid pesticide sensing in the agricultural arena: A systematic review of the current status and perspectives

Srinivasan,

Raajasubramanian,

Ashokkumar

et al. 2024

Biotech & Bioengineering

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The extensive use of chemical pesticides has significantly boosted agricultural food crop yields. Nevertheless, their excessive and unregulated application has resulted in food contamination and pollution in environmental, aquatic, and agricultural ecosystems. Consequently, the on‐site monitoring of pesticide residues in agricultural practices is paramount to safeguard global food and conservational safety. Traditional pesticide detection methods are cumbersome and ill‐suited for on‐site pesticide finding. The systematic review provides an in‐depth analysis of the current status and perspectives of nanobiosensors (NBS) for pesticide detection in the agricultural arena. Furthermore, the study encompasses the fundamental principles of NBS, the various transduction mechanisms employed, and their incorporation into on‐site detection platforms. Conversely, the assortment of transduction mechanisms, including optical, electrochemical, and piezoelectric tactics, is deliberated in detail, emphasizing its advantages and limitations in pesticide perception. Incorporating NBS into on‐site detection platforms confirms a vital feature of their pertinence. The evaluation reflects the integration of NBS into lab‐on‐a‐chip systems, handheld devices, and wireless sensor networks, permitting real‐time monitoring and data‐driven decision‐making in agronomic settings. The potential for robotics and automation in pesticide detection is also scrutinized, highlighting their role in improving competence and accuracy. Finally, this systematic review provides a complete understanding of the current landscape of NBS for on‐site pesticide sensing. Consequently, we anticipate that this review offers valuable insights that could form the foundation for creating innovative NBS applicable in various fields such as materials science, nanoscience, food technology and environmental science.

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Plasmonic Nanoparticle Conjugation for Nucleic Acid Biosensing

Cited by 2 publications

References 24 publications

High sensitivity electrochemical DNA sensors for detection of somatic mutations in FFPE samples

High sensitivity electrochemical DNA sensors for detection of somatic mutations in FFPE samples

Nanobiosensors based on on‐site detection approaches for rapid pesticide sensing in the agricultural arena: A systematic review of the current status and perspectives

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