Plastic-based acoustofluidic devices for high-throughput, biocompatible platelet separation

Gu, Yuyang; Chen, Chuyi; Wang, Zeyu; Huang, Po‐Hsun; Fu, Hai; Wang, Lin; Wu, Mengxi; Chen, Yuchao; Gao, Tieyu; Gong, Jianying; Kwun, Jean; Arepally, Gowthami M.; Huang, Tony Jun

doi:10.1039/c8lc00527c

Cited by 42 publications

(28 citation statements)

References 63 publications

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“…Therefore, W (x, z) given in Eqn (2) is simplified as sin(k 1 x) with the amplitude C m for the x − y plane cross section of glass plate and then, we apply the sinusoidal function of C m sin(k 1 x) on the glass plate for the displacement actuation of PDMS microchannel as shown in Supplementary Figure S4. This sinusoidal function is also equal to the amplitude of displacement actuation (d 0 ) presented in Eqn (8).…”

Section: Model System and Computational Domainmentioning

confidence: 99%

Flexural wave-based soft attractor walls for trapping microparticles and cells

et al. 2021

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Section: Model System and Computational Domainmentioning

confidence: 99%

Flexural wave-based soft attractor walls for trapping microparticles and cells

et al. 2021

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“…Ultrasounds have been reported as a successful method to achieve a noninvasive process of blood plasma separation based on cell enrichment processes induced by acoustic waves [28][29][30][31][32][33][34]. This label-free technology is based on a steady radiation force acoustically induced by the ultrasounds.…”

Section: Introductionmentioning

confidence: 99%

“…This current study aims to clarify and help this prediction due to the lack of experimental analyses in the literature. Various studies of the literature previous to our work describe experiments that demonstrate the ability of the ultrasounds to perform plasmapheresis in blood samples and analyze the advantages of these processes over other conventional methods [29][30][31][32][33][34]. In 2015, the authors of the current paper analyzed the influence of the hydrodynamic conditions of blood samples on the efficiency of the processes of plasma separation by ultrasounds at a fixed hematocrit percentage of Hct = 4.5%, with successful results [52].…”

Section: Introductionmentioning

confidence: 99%

Influence of Hydrodynamics and Hematocrit on Ultrasound-Induced Blood Plasmapheresis

et al. 2020

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Acoustophoretic blood plasma separation is based on cell enrichment processes driven by acoustic radiation forces. The combined influence of hematocrit and hydrodynamics has not yet been quantified in the literature for these processes acoustically induced on blood. In this paper, we present an experimental study of blood samples exposed to ultrasonic standing waves at different hematocrit percentages and hydrodynamic conditions, in order to enlighten their individual influence on the acoustic response of the samples. The experiments were performed in a glass capillary (700 µm-square cross section) actuated by a piezoelectric ceramic at a frequency of 1.153 MHz, hosting 2D orthogonal half-wavelength resonances transverse to the channel length, with a single-pressure-node along its central axis. Different hematocrit percentages Hct = 2.25%, 4.50%, 9.00%, and 22.50%, were tested at eight flow rate conditions of Q = 0:80 µL/min. Cells were collected along the central axis driven by the acoustic radiation force, releasing plasma progressively free of cells. The study shows an optimal performance in a flow rate interval between 20 and 80 µL/min for low hematocrit percentages Hct ≤ 9.0%, which required very short times close to 10 s to achieve cell-free plasma in percentages over 90%. This study opens new lines for low-cost personalized blood diagnosis.

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“…[ 35–53 ] Therefore, acoustofluidic sorters often have advantages in biocompatibility. [ 54–56 ] However, current acoustofluidic droplet sorters have deficiencies in their energy conversion rate, which hinders their further development. [ 57 ] The standard interdigital transducer design enables the bidirectional transmission of acoustic waves, causing inefficient energy utilization and excess heat.…”

Section: Introductionmentioning

confidence: 99%

Acoustofluidic Droplet Sorter Based on Single Phase Focused Transducers

Zhong

Yang

Ugolini

et al. 2021

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Droplet microfluidics has revolutionized the biomedical and drug development fields by allowing for independent microenvironments to conduct drug screening at the single cell level. However, current microfluidic sorting devices suffer from drawbacks such as high voltage requirements (e.g., >200 Vpp), low biocompatibility, and/or low throughput. In this article, a single‐phase focused transducer (SPFT)‐based acoustofluidic chip is introduced, which outperforms many microfluidic droplet sorting devices through high energy transmission efficiency, high accuracy, and high biocompatibility. The SPFT‐based sorter can be driven with an input power lower than 20 Vpp and maintain a postsorting cell viability of 93.5%. The SPFT sorter can achieve a throughput over 1000 events per second and a sorting purity up to 99.2%. The SPFT sorter is utilized here for the screening of doxorubicin cytotoxicity on cancer and noncancer cells, proving its drug screening capability. Overall, the SPFT droplet sorting device shows great potential for fast, precise, and biocompatible drug screening.

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Plastic-based acoustofluidic devices for high-throughput, biocompatible platelet separation

Abstract: A plastic-based acoustofluidic device for high-throughput platelet separation consisting of a transducer and a disposable chip.

Cited by 42 publications

References 63 publications

Flexural wave-based soft attractor walls for trapping microparticles and cells

Flexural wave-based soft attractor walls for trapping microparticles and cells

Influence of Hydrodynamics and Hematocrit on Ultrasound-Induced Blood Plasmapheresis

Acoustofluidic Droplet Sorter Based on Single Phase Focused Transducers

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