Plasticity of chemoreceptor gene expression: Sensory and circuit inputs modulate state-dependent chemoreceptors

Gruner, Matthew; Linden, Alexander M. van der

doi:10.1080/21624054.2015.1023497

Cited by 4 publications

(4 citation statements)

References 42 publications

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“…The MADS‐box gene family is ancient, and encodes a large number of transcription regulators in plants and animals. In fungi and nematodes they have an impact on pheromone response (Shore & Sharrocks, 1995; Grunera & Van der Linden, , respectively). The functions of PRTF‐like transcription factors in arthropods have not been elucidated to date.…”

Section: Resultsmentioning

confidence: 97%

Identification and gene‐silencing of a putative odorant receptor transcription factor in Varroa destructor: possible role in olfaction

Singh

Eliash

Stein

et al. 2016

Insect Molecular Biology

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The ectoparasitic mite Varroa destructor is one of the major threats to apiculture. Using a behavioural choice bioassay, we determined that phoretic mites were more successful in reaching a bee than reproductive mites, suggesting an energy trade-off between reproduction and host selection. We used both chemo-ecological and molecular strategies to identify the regulation of the olfactory machinery of Varroa and its association with reproduction. We focused on transcription regulation. Using primers designed to the conserved DNA binding region of transcription factors, we identified a gene transcript in V. destructor homologous to the pheromone receptor transcription factor (PRTF) gene of Pediculus humanus corporis. Quantitative PCR (qPCR) revealed that this PRTF-like gene transcript is expressed in the forelegs at higher levels than in the body devoid of forelegs. Subsequent comparative qPCR analysis showed that transcript expression was significantly higher in the phoretic as compared to the reproductive stage. Electrophysiological and behavioural studies revealed a reduction in the sensitivity of PRTF RNA interference-silenced mites to bee headspace, consistent with a reduction in the mites' ability to reach a host. In addition, vitellogenin expression was stimulated in PRTF-silenced mites to similar levels as found in reproductive mites. These data shed light upon the regulatory mechanism of host chemosensing in V. destructor.

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Section: Resultsmentioning

confidence: 97%

Identification and gene‐silencing of a putative odorant receptor transcription factor in Varroa destructor: possible role in olfaction

Singh

Eliash

Stein

et al. 2016

Insect Molecular Biology

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“…Subsequently, members of this new group were also found in the deer tick, Ixodes scapularis , in spiders (order Aranaea): Dysdera silvatica and Stegodyphus mimosarum , and in the genomes of the centipede Strigamia maritime (Mandibulata) 17 . As animals often alter their chemosensory input by modulating expression levels of chemosensory related genes in response to environmental and physiological changes 18 , transcriptomic studies are expected to disclose chemosensory components. This strategy was adopted in the present study.…”

Section: Introductionmentioning

confidence: 99%

Chemosensing of honeybee parasite, Varroa destructor: Transcriptomic analysis

Eliash

Singh

Thangarajan

et al. 2017

Sci Rep

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Chemosensing is a primary sense in nature, however little is known about its mechanism in Chelicerata. As a model organism we used the mite Varroa destructor, a key parasite of honeybees. Here we describe a transcriptomic analysis of two physiological stages for the Varroa foreleg, the site of primary olfactory organ. The transcriptomic analysis revealed transcripts of chemosensory related genes belonging to several groups. These include Niemann-Pick disease protein, type C2 (NPC2), gustatory receptors (GRs), ionotropic receptors (IRs), sensory neuron membrane proteins (SNMPs) and odorant binding proteins (OBP). However, no insect odorant receptors (ORs) and odorant co-receptors (ORcos) were found. In addition, we identified a homolog of the most ancient IR co-receptor, IR25a, in Varroa as well as in other members of Acari. High expression of this transcript in the mite’s forelegs, while not detectable in the other pairs of legs, suggests a function for this IR25a-like in Varroa chemosensing.

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“…In addition, starvation-mediated regulation of srh-234 expression levels in ADL is regulated by both cell- and non-cell-autonomous transcriptional mechanisms involving basic helix-loop-helix (bHLH) factors, including HLH-30 and MXL-3 acting in the intestine, and HLH-2/3 acting together with the MEF-2 factor in ADL neurons (Gruner et al 2016). Together, these findings demonstrated that expression of the srh-234 chemoreceptor gene in a single ADL sensory neuron type of C. elegans is regulated by multiple transcriptional modules, and revealed a neuron-to-intestine connection involving insulin signals in the modulation of chemoreceptor genes as a function of the C. elegans feeding state (Gruner and van der Linden 2015).…”

Section: Introductionmentioning

confidence: 90%

“…The effects of these bacterial diets on acdh-1 promoter activity have led to important insights into the vitamin B12-dependent and independent propionate breakdown pathways. C. elegans is also an ideal organism to study the plasticity in expression levels of individual chemosensory receptor genes in response to external and internal signals (Vidal et al 2018;Gruner and van der Linden 2015). Our prior study showed that the expression levels of the srh-234 chemoreceptor gene in the ADL sensory neuron type is regulated by starvation.…”

Section: Introductionmentioning

confidence: 99%

Dietary vitamin B12 regulates chemosensory receptor gene expression via the MEF2 transcription factor in Caenorhabditis elegans

McDonagh

Crew

Linden

2021

Preprint

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Dynamic changes in chemoreceptor gene expression levels in sensory neurons is one strategy that an animal can use to modify their responses to dietary changes. However, the mechanisms underlying diet-dependent modulation of chemosensory gene expression are unclear. Here, we show that the expression of the srh-234 chemoreceptor gene localized in a single ADL sensory neuron type of C. elegans is downregulated when animals are fed a Comamonas bacterial diet, but not on an E. coli diet. Remarkably, this diet-modulated effect on srh-234 gene expression levels is dependent on the micronutrient vitamin B12 endogenously produced by Comamonas bacteria. Excess propionate and genetic perturbations in the canonical and shunt propionate breakdown pathways are able to override the repressing effects of vitamin B12 on srh-234 expression. The vitamin B12-mediated regulation of srh-234 expression levels in ADL requires the MEF-2 transcription factor, providing a potential mechanism by which dietary vitamin B12 may transcriptionally tune individual chemoreceptor genes in a single sensory neuron type, which in turn may change animal responses to biologically relevant chemicals in their diet.

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Plasticity of chemoreceptor gene expression: Sensory and circuit inputs modulate state-dependent chemoreceptors

Cited by 4 publications

References 42 publications

Identification and gene‐silencing of a putative odorant receptor transcription factor in Varroa destructor: possible role in olfaction

Identification and gene‐silencing of a putative odorant receptor transcription factor in Varroa destructor: possible role in olfaction

Chemosensing of honeybee parasite, Varroa destructor: Transcriptomic analysis

Dietary vitamin B12 regulates chemosensory receptor gene expression via the MEF2 transcription factor in Caenorhabditis elegans

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