Plastid casein kinase 2 knockout reduces abscisic acid (ABA) sensitivity, thermotolerance, and expression of ABA- and heat-stress-responsive nuclear genes

Wang, Yu; Chang, Huoy-Rou; Zhou, Tianjun; Lu, Xiutao; Yuan, Congying; Zhang, Chen; Wang, Ping; Xiao, Wenjun; Xiao, Langtao; Gong, Xue; Guo, Xinhong

doi:10.1093/jxb/eru190

Cited by 66 publications

(48 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been established that ABA induces thermotolerance in wheat (Hu et al ) and rice (Zhang et al ). Also, heat‐induced damage is more severe in ABA‐deficient mutants than in their parental cultivars (Wang et al , Wu et al ). In addition, fluridone, an inhibitor of phytoene desaturase and hence of carotenoid and ABA biosynthesis, is reported to impair heat tolerance in plants under heat stress by reducing endogenous ABA level (Ding et al , Kumar et al ).…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid prevents pollen abortion under high‐temperature stress by mediating sugar metabolism in rice spikelets

Rezaul

Feng

Chen

et al. 2018

Physiologia Plantarum

114

View full text Add to dashboard Cite

Heat stress at the pollen mother cell (PMC) meiotic stage leads to pollen sterility in rice, in which the reactive oxygen species (ROS) and sugar homeostasis are always adversely affected. This damage is reversed by abscisic acid (ABA), but the mechanisms underlying the interactions among the ABA, sugar metabolism, ROS and heat shock proteins in rice spikelets under heat stress are unclear. Two rice genotypes, Zhefu802 (a recurrent parent) and fgl (its near-isogenic line) were subjected to heat stress of 40°C after pre-foliage sprayed with ABA and its biosynthetic inhibitor fluridone at the meiotic stage of PMC. The results revealed that exogenous application of ABA reduced pollen sterility caused by heat stress. This was achieved through various means, including: increased levels of soluble sugars, starch and non-structural carbohydrates, markedly higher relative expression levels of heat shock proteins (HSP24.1 and HSP71.1) and genes related to sugar metabolism and transport, such as sucrose transporters (SUT) genes, sucrose synthase (SUS) genes and invertase (INV) genes as well as increased antioxidant activities and increased content of adenosine triphosphate and endogenous ABA in spikelets. In short, exogenous application of ABA prior to heat stress enhanced sucrose transport and accelerated sucrose metabolism to maintain the carbon balance and energy homeostasis, thus ABA contributed to heat tolerance in rice.

show abstract

Section: Introductionmentioning

confidence: 99%

Abscisic acid prevents pollen abortion under high‐temperature stress by mediating sugar metabolism in rice spikelets

Rezaul

Feng

Chen

et al. 2018

Physiologia Plantarum

114

View full text Add to dashboard Cite

show abstract

“…So far, several targets of CK2, involved in plant growth, development, and abiotic stress responses, have been identified in plants (Lee et al, 1999;Riera et al, 2004;Portoles and Mas, 2007;Moreno-Romero et al, 2008). In the case of abiotic stresses, CK2 has been described as a potential enhancing factor, since ck2a mutants are hyposensitive to ABA concerning seed germination, cotyledon greening, and stomatal opening (Mulekar et al, 2012;Wang et al, 2014). These effects were attributed to the downregulation of ABA-responsive genes, including OST1 (Wang et al, 2014), but the biochemical relationship between CK2 and OST1 was unclear.…”

Section: Introductionmentioning

confidence: 99%

“…In the case of abiotic stresses, CK2 has been described as a potential enhancing factor, since ck2a mutants are hyposensitive to ABA concerning seed germination, cotyledon greening, and stomatal opening (Mulekar et al, 2012;Wang et al, 2014). These effects were attributed to the downregulation of ABA-responsive genes, including OST1 (Wang et al, 2014), but the biochemical relationship between CK2 and OST1 was unclear. By characterizing the regulation of the maize ortholog of Arabidopsis OST1, we discovered that CK2 is a direct negative regulator of ZmOST1.…”

Section: Introductionmentioning

confidence: 99%

Casein Kinase 2 Negatively Regulates Abscisic Acid-Activated SnRK2s in the Core Abscisic Acid-Signaling Module

et al. 2015

View full text Add to dashboard Cite

SnRK2 kinases, PP2C phosphatases and the PYR/PYL/RCAR receptors constitute the core abscisic acid (ABA) signaling module that is thought to contain all of the intrinsic properties to self-regulate the hormone signal output. Here we identify Casein Kinase (CK)2 as a novel negative regulator of SnRK2. CK2 phosphorylates a cluster of conserved serines at the ABA box of SnRK2, increasing its binding to PP2C and triggering protein degradation. Consequently, CK2 action has implications on SnRK2 protein levels, as well as kinase activity and its response to abiotic stimuli.

show abstract

“…Up transcripted SLG1 plays a role in regulating the entering or exiting the cell cycle and has a functional link between mitochondrial RNA editing and responses to abiotic stress [57]. Casein kinase II subunit alpha (CKA1) was up regulated at mRNA level and is an enhancing factor in abiotic stress signaling via modulating the expression of some molecular elements in retrograde signaling [58]. Transcriptically up regulated UBP3-associated protein BRE5 (BRE5) is involved in the formation of stress granules appearing when cells are under stress [59].…”

Section: Stress Mediation and Antioxidative Defensesmentioning

confidence: 99%

Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis

Zhang

et al. 2020

Microorganisms

View full text Add to dashboard Cite

Trichothecium roseum is a harmful postharvest fungus causing serious damage, together with the secretion of insidious mycotoxins, on apples, melons, and other important fruits. Cuminal, a predominant component of Cuminum cyminum essential oil has proven to successfully inhibit the growth of T. roseum in vitro and in vivo. Electron microscopic observations revealed cuminal exposure impaired the fungal morphology and ultrastructure, particularly the plasmalemma. Transcriptome and proteome analysis was used to investigate the responses of T. roseum to exposure of cuminal. In total, 2825 differentially expressed transcripts (1516 up and 1309 down) and 225 differentially expressed proteins (90 up and 135 down) were determined. Overall, notable parts of these differentially expressed genes functionally belong to subcellular localities of the membrane system and cytosol, along with ribosomes, mitochondria and peroxisomes. According to the localization analysis and the biological annotation of these genes, carbohydrate and lipids metabolism, redox homeostasis, and asexual reproduction were among the most enriched gene ontology (GO) terms. Biological pathway enrichment analysis showed that lipids and amino acid degradation, ATP-binding cassette transporters, membrane reconstitution, mRNA surveillance pathway and peroxisome were elevated, whereas secondary metabolite biosynthesis, cell cycle, and glycolysis/gluconeogenesis were down regulated. Further integrated omics analysis showed that cuminal exposure first impaired the polarity of the cytoplasmic membrane and then triggered the reconstitution and dysfunction of fungal plasmalemma, resulting in handicapped nutrient procurement of the cells. Consequently, fungal cells showed starvation stress with limited carbohydrate metabolism, resulting a metabolic shift to catabolism of the cell's own components in response to the stress. Additionally, these predicaments brought about oxidative stress, which, in collaboration with the starvation, damaged certain critical organelles such as mitochondria. Such degeneration, accompanied by energy deficiency, suppressed the biosynthesis of essential proteins and inhibited fungal growth.fungicides to be used for postharvest treatment, reduced efficacy due to the emergence of pathogen resistance to these chemicals, and the growing public concerns over chemical residues in food and the environment have necessitated the seeking of alternative solutions to control the fungal spoilage and to guarantee food safety and consumer health [8].Essential oils (EOs), generally recognized as safe by the US Food and Drug Administration [9], are natural substances with established antimicrobial activities [10,11] and are a candidate instrument for controlling postharvest disease [12,13]. Nonetheless, this candidacy is compromised by the expensiveness of these natural substances due to their generally low yields. However, exploring their mode of actions is valuable to the development of novel alternatives.The multicomponent EOs attribute their mode of act...

show abstract

Plastid casein kinase 2 knockout reduces abscisic acid (ABA) sensitivity, thermotolerance, and expression of ABA- and heat-stress-responsive nuclear genes

Abstract: SummaryThis study demonstrated a role of plastid CK2 in ABA and heat-stress signalling. Its knockout led to reduced ABA sensitivity, thermotolerance, and expression of nuclear genes involved in these processes.

Cited by 66 publications

References 77 publications

Abscisic acid prevents pollen abortion under high‐temperature stress by mediating sugar metabolism in rice spikelets

Abscisic acid prevents pollen abortion under high‐temperature stress by mediating sugar metabolism in rice spikelets

Casein Kinase 2 Negatively Regulates Abscisic Acid-Activated SnRK2s in the Core Abscisic Acid-Signaling Module

Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis

Contact Info

Product

Resources

About