Plastids with densely staining thylakoid contents inNymphoides indica

Steveninck, M. E. Van; Steveninck, R. F. M. Van

doi:10.1007/bf01276961

Cited by 14 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Van Steveninck and Van Steveninck (1980 a , b ) reported that, in the lower epidermal and sub-epidermal cells in leaves of Nymphoides indica , thylakoids have densely electron opaque loculi. The substance filling the thylakoidal lumen only stained when glutaraldehyde prefixation preceded osmium tetroxide treatment, suggesting that this unknown substance forms a stainable complex with the aldehyde; additional tests revealed its hydrophilic and oxidizable nature.…”

Section: Discussionmentioning

confidence: 99%

Phenol homeostasis is ensured in vanilla fruit by storage under solid form in a new chloroplast-derived organelle, the phenyloplast

Brillouet

Verdeil

Odoux

et al. 2014

Journal of Experimental Botany

View full text Add to dashboard Cite

A multiple cell imaging approach combining immunofluorescence by confocal microscopy, fluorescence spectral analysis by multiphotonic microscopy, and transmission electron microscopy identified the site of accumulation of 4-O-(3-methoxybenzaldehyde) β-d-glucoside, a phenol glucoside massively stockpiled by vanilla fruit. The glucoside is sufficiently abundant to be detected by spectral analysis of its autofluorescence. The convergent results obtained by these different techniques demonstrated that the phenol glucoside accumulates in the inner volume of redifferentiating chloroplasts as solid amorphous deposits, thus ensuring phenylglucoside cell homeostasis. Redifferentiation starts with the generation of loculi between thylakoid membranes which are progressively filled with the glucoside until a fully matured organelle is obtained. This peculiar mode of storage of a phenolic secondary metabolite is suspected to occur in other plants and its generalization in the Plantae could be considered. This new chloroplast-derived organelle is referred to as a ‘phenyloplast’.

show abstract

Section: Discussionmentioning

confidence: 99%

Phenol homeostasis is ensured in vanilla fruit by storage under solid form in a new chloroplast-derived organelle, the phenyloplast

Brillouet

Verdeil

Odoux

et al. 2014

Journal of Experimental Botany

View full text Add to dashboard Cite

show abstract

“…We propose that accumulation of a DLS in the thylakoid lumen, as revealed by inverse staining, is an important mechanism in Haberlea. Although the DLS has not been chemically identified, on the basis of fixation, staining and extraction probes it is probably a phenolic compound (van Steveninck and van Steveninck, 1980;Keresztes and Sárvári, 2001a). Some phenolics are considered to be antioxidants (Sgherri et al, 2004) and ROS scavengers (Kruk et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Protection of thylakoids against combined light and drought by a lumenal substance in the resurrection plant Haberlea rhodopensis

Georgieva¹,

Sárvári²,

Keresztes³

2009

Annals of Botany

View full text Add to dashboard Cite

Accumulation of DLS (possibly phenolics) in the thylakoid lumen is demonstrated and is proposed as a mechanism protecting the thylakoid membranes of H. rhodopensis during desiccation and recovery under LL. Disappearance of DLS during desiccation in ML could leave the thylakoid membranes without protection, allowing oxidative damage during dehydration and the initial rehydration, thus preventing recovery of photosynthesis.

show abstract

“…At the same time, performed cytochemical analysis of the content of these thylakoids did not reveal that electron-dense substance in them is represented by compounds such as proteins, lipids or polysaccharides (Van Steveninck & Van Steveninck 1980b). Despite many experimental efforts made in this direction in order to elucidate a nature of this substance, this problem has still remained to be unresolved.…”

Section: Introductionmentioning

confidence: 99%

“…As far back as in late 1960s, in the course of studies of chloroplast ultrastructure in some plant species a number of researchers could observe unusual, electrondense thylakoids by using of transmission electron microscopy (Stetler & Laetsch 1969;Van Steveninck & Van Steveninck 1980a;Semenova 1985;Semenova 2005). At the same time, performed cytochemical analysis of the content of these thylakoids did not reveal that electron-dense substance in them is represented by compounds such as proteins, lipids or polysaccharides (Van Steveninck & Van Steveninck 1980b).…”

Section: Introductionmentioning

confidence: 99%

Study of electron-dense thylakoids in chloroplasts of Stevia rebaudiana Bertoni in relation to the biosynthesis of diterpenoids

et al. 2013

View full text Add to dashboard Cite

Using transmission electronic microscopy and mass spectrometry electron-dense thylakoids of chloroplasts of Stevia rebaudiana leaves during active vegetable growth of this plant were studied in relation to the biosynthesis of diterpenoid glycosides (DGs). It was found that these compounds are absent in these thylakoids, but they contain a water-insoluble weakly polar ent-kauren, a known biosynthetic precursor of DGs as well as gibberellins. This finding provides a base for the suggestion that similar, electron-dense, thylakoids were observed earlier by other authors in other plant species. These data allowed us to conclude that an intensive biosynthesis of ent-kauren is likely related to adaptation of the short-day plants including Stevia rebaudiana to vegetable growth under the long day conditions.

show abstract

Plastids with densely staining thylakoid contents inNymphoides indica

Cited by 14 publications

References 49 publications

Phenol homeostasis is ensured in vanilla fruit by storage under solid form in a new chloroplast-derived organelle, the phenyloplast

Phenol homeostasis is ensured in vanilla fruit by storage under solid form in a new chloroplast-derived organelle, the phenyloplast

Protection of thylakoids against combined light and drought by a lumenal substance in the resurrection plant Haberlea rhodopensis

Study of electron-dense thylakoids in chloroplasts of Stevia rebaudiana Bertoni in relation to the biosynthesis of diterpenoids

Contact Info

Product

Resources

About