Platelet-activating factor in normal rat uterus

Yasuda, Katsuhiko; Satouchi, Kiyoshi; Saito, Kunihiko

doi:10.1016/s0006-291x(86)80414-4

Cited by 63 publications

(23 citation statements)

References 17 publications

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“…However, PAF in the hormonally stimulated rabbit uterus reached levels as high as 60 pmol/g wet weight of tissue, depending on the time elapsing before processing (see Table 1) and the day of pregnancy or pseudopregnancy. Concentrations of PAF in the rabbit uterus therefore approach levels that are similar to those reported by Yasuda et al (1986) for the rat.…”

Section: Introductionsupporting

confidence: 84%

“…These findings therefore suggest that uterine PAF plays an important role in early events leading to successful implantation of the embryo. PAF has also been found in the non-pregnant rat uterus, a total PAF content of 21-3 ng/uterus being reported (Yasuda et al, 1986). Assuming an average uterine wet weight of 500 mg, this is equivalent to 42-6 ng PAF/g wet weight of tissue or 81-3 pmol/g.…”

Section: Introductionmentioning

confidence: 97%

“…Assuming an average uterine wet weight of 500 mg, this is equivalent to 42-6 ng PAF/g wet weight of tissue or 81-3 pmol/g. Yasuda et al (1986) also indicated that rabbit uteri contained PAF but in much smaller quantities (no data were given). In the studies presented here, PAF concentrations in oestrous rabbit uteri were~2 pmol/g wet weight, a value which is indeed considerably lower than that reported for the oestrous uterus of the rat.…”

Section: Introductionmentioning

confidence: 99%

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Platelet-activating factor in the rabbit uterus during early pregnancy

Angle

Jones

McManus³

et al. 1988

Reproduction

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Summary. Platelet-activating factor (PAF) concentrations were low in the non-pregnant, oestrous uterus (mean \m=+-\s.e.m.: 2\m=.\2\m=+-\1\m=.\2 pmol/g, n = 3). However, uterine PAF increased dramatically during pregnancy to a maximum of 37\m=.\8\ m=+-\ 4\m=.\90pmol/g (n = 7) on Day 5. By Day 7, PAF concentrations in the uteri of pregnant rabbits had returned to levels similar to those found at oestrus. In contrast, uterine PAF in pseudopregnant rabbits peaked at 30\m=.\6\ m=+-\2\m=.\8pmol/g (n = 8) on Day 4, declined to 20\m=.\5\ m=+-\ 2\ m=. \ 4pmol/g (n = 8) on Day 5 and then remained at that concentration through Day 7. Uterine PAF co-migrated with synthetic PAF 1-0-hexadecyl-2-acetyl-sn-glycero-phosphocholine) in both thin-layer and normal-phase high-performance liquid chromatography. PAF activity in the uterus during pregnancy and pseudopregnancy was found almost exclusively in the endometrium; little or no PAF was found in myometrium, uterine flushings or blastocysts. While no PAF was detected in blastocysts on Days 5 and 6 of pregnancy, the presence of the embryo appears to modulate biosynthesis and/or degradation of PAF by the uterus, since PAF decreased significantly in uterine tissue apposed to the implanting embryo (but not in similar areas between such attachment sites). Increased concentrations of PAF in the preimplantation rabbit uterus followed by a dramatic decrease on the day of blastocyst attachment suggest that this potent inflammatory autacoid may play a vital role in implantation.

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Section: Introductionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Platelet-activating factor in the rabbit uterus during early pregnancy

Angle

Jones

McManus³

et al. 1988

Reproduction

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“…However, it is also produced by a number of nonimmune cells such as endothelial (9), vascular, muscle (43), glomerular (13) and endometrial cells (4,5,7,45). PAF plays a role in many reproductive events (16), including ovulation (1), fertilization (27), implantation and early development of embryo (28).…”

Section: Introductionmentioning

confidence: 99%

“…PAF plays a role in many reproductive events (16), including ovulation (1), fertilization (27), implantation and early development of embryo (28). PAF has been determined in the uterus of rats (45), rabbits (5), dogs (35) and humans (4). It plays a significant role in mammalian reproduction (29,32).…”

Section: Introductionmentioning

confidence: 99%

Expression of platelet activating factors and its receptor in cat uterus during early pregnancy

Ağaoğlu¹,

Ağaoğlu²,

Kurar³

et al. 2013

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:Platelet activating factors (PAF1β, PAF1γ, PAF:ah, PAF-R) have a significant role during mammalian pregnancy process. This study aimed to investigate expression profiles of PAF gene family in cat uterus during pregnancy. Tissue samples were collected via ovariohysterectomy at days 7 (Gr1; n=3), 18-20 (Gr2; n=7), 24-25 (Gr3; n=5) after mating from the different regions of the uterus. Relative expression levels of genes were quantified using real-time qRT-PCR. A mixed model was fitted on the normalized data and Least Significant Difference (LSD) test was employed to determine significant differences between groups. Expressions of PAF genes were assessed in the cat uterus during pregnancy around implantation period; however, no significant differences were detected between the preimplantation and the implantation stage. Similarly, no significant difference concerning mRNA expression was assessed between different parts of the uterus during pregnancy. In conclusion, PAF genes are expressed in the cat uterus during early pregnancy. However, this expression is not associated with changes in the uterus during early pregnancy.Key words: PAF, cat, pregnancy, qRT-PCR. Kedi uterusunda, platelet aktive edici faktörler ve reseptörünün erken gebelik dönemindeki ekspresyonuÖzet: Platelet aktive edici faktörler (PAF1β, PAF1γ, PAF:ah, PAF-R), memeli hayvanların gebelik sürecinde önemli bir role sahiptir. Bu çalışmanın amacı, gebelik süresince kedi uterusunda PAF gen ailesi ekspresyon profillerini araştırmaktır. Doku örnekleri çiftleşme sonrası 7 (Gr1; n=3), 18-20 (Gr2; n=7), 24-25. (Gr3; n=5) günlerde yapılan ovariyohisterektomi operasyonları ile uterusun farklı bölgelerinden toplandı. Nispi mRNA ekspresyon seviyeleri real-time PZR kullanılarak tespit edildi. Normalize edilmiş veriler karışık model kullanılarak analiz edildi ve istatistikî olarak farklı olan gruplar Asgari Önemli Fark (AÖF) testi ile tespit edildi. Kedi uterusunda implantasyon sürecinde PAF genlerinin eksprese olduğu gözlenmiştir ancak; istatistikî olarak çalışma grupları ve uterus bölgeleri arasında herhangi bir fark bulunmamıştır. Sonuç olarak, kedilerde erken gebelik sürecinde uterusta PAF genleri eksprese olmaktadır. Ancak; bu ekspresyonların erken gebelik döneminde uterusta görülen değişikliklerle ilişkisi yoktur.

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Platelet-activating factor acetylhydrolase in seminal plasma: A brief review

Soubeyrand

Manjunath

1998

Mol. Reprod. Dev.

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Platelet-activating factor in normal rat uterus

Cited by 63 publications

References 17 publications

Platelet-activating factor in the rabbit uterus during early pregnancy

Platelet-activating factor in the rabbit uterus during early pregnancy

Expression of platelet activating factors and its receptor in cat uterus during early pregnancy

Platelet-activating factor acetylhydrolase in seminal plasma: A brief review

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