Platelet adhesion on laser‐induced acrylic acid–grafted polyethylene terephthalate

Abstract

ABSTRACT:To improve blood compatibility, acrylic acid (AAc) was grafted onto a polyethylene terephtalate (PET) film surface using lasers. The PET surface was irradiated with a CO 2 pulsed laser, and then graft copolymerization was carried out in an aqueous solution of AAc in the presence of Mohr's salt. Different techniques such as attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), and contact angle measurements were used to characterize the modified PET surface. The ATR-FTIR spectra confirmed the creation of new functional groups on the PET surface, and contact angle measurements revealed that the hydrophilicity of the PET surface increased as a result of the AAc graft polymerization. The electron micrographs showed that the grafting changed the surface morphology of the PET film. To evaluate the blood compatibility in vitro, the number of platelets adhering to the modified PET surface was determined using lactate dehydrogenase (LDH) activity measurement. The data from LDH method indicated that the extent of platelet adherence on the unmodified PET was much higher than that on the AAc grafted PET. The morphology of adhered platelets on the PET surface was investigated by SEM. The results showed that platelet adhesion and activation onto the PET surface was reduced because of AAc graft polymerization.

Biomimetic modification of silicone tubes using sodium nitrite–collagen immobilization accelerates endothelialization

“…AAc grafting increases material surface hydrophilicity, thereby preventing platelet adhesion. 36 Collagen is highly thrombogenic and induces platelet adhesion on a material surface. 11 Our data agree with these observations, because we found reduced platelet adhesion on AAc Si tubes and increased platelet adhesion on AAc Si-Col tubes.…”

Development of a New Polypropylene‐Based Suture: Plasma Grafting, Surface Treatment, Characterization, and Biocompatibility Studies

“…Consequently R (%) is the percentage reduction ratio which indicates antimicrobial efficiency. [29] In Vitro Studies 3T3 Swiss mouse fibroblasts were cultured in Dulbeccos's Modified Eagle's Medium (DMEM, Invitrogen) containing with 10% fetal calf serum (10% FCS-DMEM) and 1% antibiotics (penicillin-streptomycin, Invitrogen) at 37 8C under a humidified atmosphere in a CO 2 (5%) incubator (ASSAB T304 GF). The media was changed every 3 days.…”

“…[35] Therefore, poly(acrylic acid) is a negatively charged polymer and its grafting on surfaces allows poor cell adhesion. Mirzadeh [29] showed the dependence on the platelet adhesion depending on the acrylic acid graft density on polyethylene terephtalate surfaces. Dos Santos et al [31] developed membranes by graft copolymerization of acrylic acid and 2-hydroxyethyl methacrylate (HEMA) onto chitosan.…”

Pretreatments of Textiles Prior to Dyeing: Plasma Processing

“…In particular, the FTIR spectrum shows that the film contains a high density of C(O)OH groups. The absorption peaks assignment is given in table below (Alaa et al, 2011;Cho et al, 1990;Chilcoti and Ratner, 1993;Eun-Young Choi and Seung-Hyeon Moon, 2007;Jafari et al, 2006;Mirzadeh et al, 2002 2800 to 3800 a broad OH stretching absorption band due to monomeric and dimeric C(O)OH Table 1. IR peak assignment of PPAA It has been observed that our result of plasma polymerization of acrylic acid is in relevance with the literature.…”