PLATELET AGGREGATION IN PATIENTS BITTEN BY THE BRAZILIAN SNAKE Bothrops jararaca

Sano-Martins, Ida Sigueko; Santoro, Marcelo Larami; Castro, S. C. B.; Fan, Hui Wen; Cardoso, João Luiz Costa; Theakston, R.D.G.

doi:10.1016/s0049-3848(97)00118-7

Cited by 46 publications

(24 citation statements)

References 32 publications

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“…Blood was diluted (1:10) with 3.8% Na-citrate. Platelet-rich plasma (PRP) was obtained after centrifugation of the diluted blood at 190 g for 15 min at 25 C. The remaining portion of the blood was centrifuged at 1700Â g for 15 min at 4 C to obtain platelet poor plasma (PPP) (Sano-Martins et al, 1997). Final platelet count in PRP was adjusted to 300 Â 10 3 /ml by dilution with PPP.…”

Section: Platelet Aggregationmentioning

confidence: 99%

Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Mitra

Bhattacharyya

2014

Toxicon

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Section: Platelet Aggregationmentioning

confidence: 99%

Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Mitra

Bhattacharyya

2014

Toxicon

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“…Rats experimentally envenomed presented in vivo platelet hypoaggregation caused by intravascular generation of nitric oxide and fibrin/fibrinogen degradation products, though the platelet counts were only partially reduced (26%) [12]. Quite similarly, platelet hypoaggregation was also observed in Bothrops jararaca envenomation, however with more accentuated thrombocytopenia, where platelet counts reduced approximately to 50% [26,27].…”

Section: Discussionmentioning

confidence: 98%

Lonomia obliqua venomous secretion induces human platelet adhesion and aggregation

Berger

Reck

Terra

et al. 2010

J Thromb Thrombolysis

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The caterpillar Lonomia obliqua is a venomous animal that causes numerous accidents, especially in southern Brazil, where it is considered a public health problem. The clinical manifestations include several haemostatic disturbances that lead to a hemorrhagic syndrome. Considering that platelets play a central role in hemostasis, in this work we investigate the effects of L. obliqua venomous secretion upon blood platelets responses in vitro. Results obtained shows that L. obliqua venom directly induces aggregation and ATP secretion in human washed platelets in a dose-dependent manner. Electron microscopy studies clearly showed that the venomous bristle extract was also able to produce direct platelets shape change and adhesion as well as activation and formation of platelet aggregates. Differently from other enzyme inhibitors, the venom-induced platelet aggregation was significatively inhibited by p-bromophenacyl bromide, a specific inhibitor of phospholipases A2. Additional experiments with different pharmacological antagonists indicate that the aggregation response triggered by the venom active components occurs through a calcium-dependent mechanism involving arachidonic acid metabolite(s) of the cyclooxygenase pathway and activation of phosphodiesterase 3A, an enzyme that leads to the consumption of intracellular cAMP content. It was additionally found that L. obliqua-induced platelet aggregation was independent of ADP release. Altogether, these findings are in line with the need for a better understanding of the complex hemorrhagic syndrome resulting from the envenomation caused by L. obliqua caterpillars, and can also give new insights into the management of its clinical profile.

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“…All venom nucleotidases isolated so far are glycoproteins [47,48]. This implies that the presence of a sugar part would allow the binding of this protein to the glycocalix directing enzyme action to the membrane structure, facilitate its effects on purine receptors acting as an exogenous ectonucleotidase (49)(50).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Novel ADPase in Bothrops jararaca Snake Venom Involved in Nucleotide Hydrolysis

Teixeira-Ferreira

Alves²

2007

PPL

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Snake venoms hydrolyze several phosphorylated substrates. However, not is clearly understood which enzyme(s) is (are) involved in these process. Here, we propose the existence of an independent ADPase activity. In addition, we studied the reactions mechanism of nucleotide hydrolysis. This system resembles membrane ecto-nucleotidases and acts with a multi enzymatic complex transforming ATP in adenosine without the accumulation of intermediates.

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PLATELET AGGREGATION IN PATIENTS BITTEN BY THE BRAZILIAN SNAKE Bothrops jararaca

Cited by 46 publications

References 32 publications

Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Lonomia obliqua venomous secretion induces human platelet adhesion and aggregation

Characterization of a Novel ADPase in Bothrops jararaca Snake Venom Involved in Nucleotide Hydrolysis

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