Platelet Antibody Analysis by Three Different Tests

Sareban, Nazanin; Macher, Susanne; Drexler, Camilla; Posch, Ursula; Lanzer, Gerhard; Schallmoser, Katharina

doi:10.1002/jcla.21750

Cited by 5 publications

(7 citation statements)

References 16 publications

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“…False‐positive results using this assay also reportedly have occurred as a consequence of human anti‐mouse antibodies . False‐positive results also may have occurred because of low positive OD cutoff thresholds, as suggested by others . Of note, in January 2016, the cutoff OD thresholds to define positive results were increased for the LIFECODES PakPlus screen.…”

Section: Discussionmentioning

confidence: 84%

“…Alternatively, the conflicting results may be due to the different methods used for platelet antibody detection. Different platelet antibody detection assays can yield highly divergent results . The earlier study employed the Capture‐P Ready Screen and only estimated platelet‐specific glycoprotein immunization by attempting to elute HLA class I antigens with chloroquine treatment.…”

Section: Discussionmentioning

confidence: 99%

“…25 False-positive results also may have occurred because of low positive OD cutoff thresholds, as suggested by others. 26 Of note, in January 2016, the cutoff OD thresholds to define positive results were increased for the LIFECODES PakPlus screen. Using these new thresholds in our study, fewer patients would be classified as having platelet-specific glycoprotein antibodies, but there still would be no significant difference in the prevalence of antibody between the transfused (6 of 90 patients; 7%) and never transfused (3 of 24 patients; 13%) groups.…”

Section: Discussionmentioning

confidence: 99%

“…Different platelet antibody detection assays can yield highly divergent results. 26 The earlier study employed the Capture-P Ready Screen and only estimated platelet-specific glycoprotein immunization by attempting to elute HLA class I antigens with chloroquine treatment. The LIFECODES PakPlus test used in the current study more directly measured reactivity to specific platelet glycoproteins.…”

Section: Discussionmentioning

confidence: 99%

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Leukoreduced red blood cell transfusions do not induce platelet glycoprotein antibodies in patients with sickle cell disease

et al. 2016

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Leukocyte-reduced RBC transfusions do not appear to induce platelet-specific glycoprotein antibodies. The positive platelet-specific glycoprotein antibody results from this study may represent platelet autoantibodies, platelet alloantibodies, or false-positive reactions. A better understanding of the immunobiology of patients with SCD at baseline and after blood product exposure may help improve future transfusion and transplantation.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Leukoreduced red blood cell transfusions do not induce platelet glycoprotein antibodies in patients with sickle cell disease

et al. 2016

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“…Such an approach has a very low sensitivity and is always inferior to a direct GPspecific test. 33,34 However, in some ITP patients with very low platelet counts, a direct test cannot be performed, and a positive indirect test may be considered helpful.…”

Section: Indirect Glycoprotein-specific Assaysmentioning

confidence: 99%

Diagnosing Immune Thrombocytopenia

Sachs

2019

Hamostaseologie

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Although the detection of a characteristic autoantibody can prove immune thrombocytopenia (ITP), this diagnosis is often based on the exclusion of other causes of thrombocytopenia. Direct glycoprotein (GP)-specific tests have the property required to demonstrate such a characteristic autoantibody. In contrast, platelet-associated immunoglobulin G or antibody detection in plasma or serum is an insufficient diagnostic test. Moreover, data for commercial capture assays are sparse and their use is currently not recommended. A significant drawback of direct GP-specific tests is their low sensitivity, and a negative test result has no relevance. It is therefore also useful to establish a diagnosis of (primarily) hyperdestructive thrombocytopenia. A full blood count together with the immature platelet fraction has an excellent positive predictive value for ITP. Plasma glycocalicin has no apparent diagnostic value in identifying ITP patients, and conflicting data for TPO preclude its use for diagnostic purposes.

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