Platelet Factor 3 — Properties and Clinical Significance

Gaetano, G. de; Vermylen, Jos; Verstraete, Marc

doi:10.1007/978-1-4684-3231-2_9

Cited by 10 publications

(1 citation statement)

References 17 publications

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“…During platelet secretion, several procoagulant and anti-coagulant factors as well as polyphosphates supporting factor XII activation are released [32,33]. In addition, strong platelet activation also foster coagulation via membrane changes rendering factors such as platelet factor 3 (PF3) available for interacting with plasmatic coagulation factors [34]. Platelet P-selectin upregulates tissue factor expression/release by monocytes [35] and is able to bind PSGL-1 on TF-positive extracellular vesicles [36] such that thrombin generation can therefore be accelerated [37] and coagulation can be fostered.…”

Section: Discussionmentioning

confidence: 99%

Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study

Izzi

Gialluisi

Gianfagna

et al. 2021

Cells

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Defined as an index of platelet size heterogeneity, the platelet distribution width (PDW) is still a poorly characterized marker of platelet function in (sub)clinical disease. We presently validated PDW as a marker of P-selectin dependent platelet activation in the Moli-family cohort. Platelet-bound P-selectin and platelet/leukocyte mixed aggregates were measured by flow cytometry in freshly collected venous blood, both before and after in vitro platelet activation, and coagulation time was assessed in unstimulated and LPS- or TNFα-stimulated whole blood. Closure Times (CT) were measured in a Platelet Function Analyzer (PFA)-100. Multivariable linear mixed effect regression models (with age, sex and platelet count as fixed and family structure as random effect) revealed PDW to be negatively associated with platelet P-selectin, platelet/leukocyte aggregates and von Willebrand factor (VWF), and positively with PFA-100 CT, and LPS- and TNF-α-stimulated coagulation times. With the exception of VWF, all relationships were sex-independent. In contrast, no association was found between mean platelet volume (MPV) and these variables. PDW seems a simple, useful marker of ex vivo and in vitro P-selectin dependent platelet activation. Investigations of larger cohorts will define the usefulness of PDW as a risk predictor of thrombo-inflammatory conditions where activated platelets play a contributing role.

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Section: Discussionmentioning

confidence: 99%

Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study

Izzi

Gialluisi

Gianfagna

et al. 2021

Cells

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Localization of polysaccharides, proteins and lipids in human and pig blood platelets

Morgenstern

Weber

1974

Histochemistry

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Lack of platelet factor-3 activation after incubation of platelet-rich plasma with kaolin in the rat

1976

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Stypven times, measured in rat platelet-rich plasma (P.R.P.) after incubation with kaolin, did not shorten as incubation proceeded, thus reflecting the lack of development of platelet factor-3 (PF3) availability in this test system. Repeated freezing and thawing of P.R.P. or aggregation with collegan did result in PF-3 availability. Aggregation and PF-3 availability were inhibited by the compound VK774. These findings add another aspect to the list of species differences in platelet function.

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Platelet Factor 3 — Properties and Clinical Significance

Cited by 10 publications

References 17 publications

Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study

Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study

Localization of polysaccharides, proteins and lipids in human and pig blood platelets

Lack of platelet factor-3 activation after incubation of platelet-rich plasma with kaolin in the rat

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