Introduction: Urinary Tract infection (UTI) is considered as the most common infections disease among women. UTI may lead to a serious complication in both kidneys. In this study, we tried to determine whether the alternation in platelet count during UTI is related to specific Gram-type of bacteria causing the infection. This assists the medical staff in selection of suitable antimicrobial drug. Material and Methods: Data of all urine cultures from Gynecology Department/ Albaraa Hospital/Tripoli processed at the Laboratory during a period of three years (2017 - 2019). All Data analysis and statistics were made with the SPSS software version 16 and performed with one-way ANOVA and a parametric test was used for categorical values and not normally distributed values. Results: A growth was detected in 101 of 193 (52%) samples and 22 (11%) were mixed growth. Gram-negative bacteria identified in 57% (45/79) while 43% (34/79) occurred with Gram-positive bacteria. Regarding to platelets count alteration, it is revealed that there was a significant difference between cases infected by Gram-negative bacteria compared to others infected by Gram-positive bacteria and control group. Conclusions: Results of the current study suggest that the change in platelet count during UTI is associated with causative bacteria’s Gram-type.