Platelet microparticle generation assay for heparin-induced thrombocytopenia diagnosis: How should we express the results?

Minet, Valentine; Bailly, Nicolas; Dogné, Jean‐Michel; Mullier, François

doi:10.1016/j.thromres.2015.04.035

Cited by 6 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In 2000, Hughes et al (21) described the MPs production process following the incubation of HIT sera with washed healthy platelets, using electron microscopy. Moreover, based on these demonstrations, Mullier and Ker enyi et al (37)(38)(39)(40) recently proposed a new flow-cytometric assay to improve the diagnosis of HIT measuring the levels of platelets-derived/Annexin1 MPs generated in the plasma of a healthy donor after the incubation with HITsuspected sera and different concentrations of heparin. Here we confirmed in plasma from patients with HIT the presence of circulating MPs derived from activated platelets and in particular, MPs carrying PF4.…”

Section: Discussionmentioning

confidence: 99%

Activated Platelet‐Derived and Leukocyte‐Derived Circulating Microparticles and the Risk of Thrombosis in Heparin‐Induced Thrombocytopenia: A Role for PF4‐Bearing Microparticles?

Campello

Radu

Duner

et al. 2017

Cytometry Part B Clinical

View full text Add to dashboard Cite

We showed for the first time the presence of circulating PF4-bearing MPs derived from activated platelets in patients with HIT; activated leukocyte/TF + MPs are associated with an increased thrombotic risk. Our findings confirm that HIT antibodies complexes may determine a TF-driven prothrombotic state through the activation of platelets and leukocytes. © 2017 International Clinical Cytometry Society.

show abstract

Section: Discussionmentioning

confidence: 99%

Activated Platelet‐Derived and Leukocyte‐Derived Circulating Microparticles and the Risk of Thrombosis in Heparin‐Induced Thrombocytopenia: A Role for PF4‐Bearing Microparticles?

Campello

Radu

Duner

et al. 2017

Cytometry Part B Clinical

View full text Add to dashboard Cite

show abstract

“…After the incubation step, platelet mixture is incubated with a fluorescent-labelled antibody to identify platelets and PMPs such as anti-CD41 [ 51 , 55 , 56 , 57 ] or anti-GPIbα [ 58 ], the latter monoclonal antibody links a subunit of the von Willebrand factor receptor complex (GPIb–IX–V) expressed on the surface of platelets and PMPs [ 19 ]. Annexin-V may be added to bind anionic phospholipids as a platelet activation marker [ 51 , 55 , 56 , 57 ]. PMPs are distinguished from platelets by their size and scatter parameters.…”

Section: Activation Endpointsmentioning

confidence: 99%

“…Studies showed good correlation between FCA and SRA [ 48 , 49 ], HIPA [ 50 , 95 ] or final clinical HIT diagnosis [ 124 ]. Flow cytometry measuring PMPs was evaluated as a functional assay [ 51 , 55 , 56 , 57 , 58 ]. Washed platelets [ 58 ], whole blood [ 55 , 56 , 57 ] or PRP [ 51 ] were used.…”

Section: Existing Functional Assays and Their Characteristicsmentioning

confidence: 99%

“…Flow cytometry measuring PMPs was evaluated as a functional assay [ 51 , 55 , 56 , 57 , 58 ]. Washed platelets [ 58 ], whole blood [ 55 , 56 , 57 ] or PRP [ 51 ] were used. Studies showed that PMPs as a platelet activation endpoint gave comparable results with SRA [ 55 , 56 , 58 ].…”

Section: Existing Functional Assays and Their Characteristicsmentioning

confidence: 99%

See 1 more Smart Citation

Functional Assays in the Diagnosis of Heparin-Induced Thrombocytopenia: A Review

Minet

Dogné

Mullier³

2017

Molecules

Self Cite

View full text Add to dashboard Cite

A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis.

show abstract

“…Future assays to potentially supplant SRA include newer HPLC methods, although such procedures are similarly complex and also require specialized expertise and equipment. More likely to continue to evolve as functional assays are whole blood aggregation (especially the Multiplate procedure) and flow cytometry methods; however, standardization issues, instrumentation requirements and potential for lower sensitivity for pathological HIT compared to SRA may delay their more comprehensive take up.…”

Section: Future Perspectivementioning

confidence: 99%

Laboratory tests for identification or exclusion of heparin induced thrombocytopenia: HIT or miss?

Favaloro

2017

American J Hematol

View full text Add to dashboard Cite

Heparin induced thrombocytopenia (HIT) is a potentially fatal condition that arises subsequent to formation of antibodies against complexes containing heparin, usually platelet-factor 4-heparin ("anti-PF4-heparin"). Assessment for HIT involves both clinical evaluation and, if indicated, laboratory testing for confirmation or exclusion, typically using an initial immunological assay ("screening"), and only if positive, a secondary functional assay for confirmation. Many different immunological and functional assays have been developed. The most common contemporary immunological assays comprise enzyme-linked immunosorbent assay [ELISA], chemiluminescence, lateral flow, and particle gel techniques. The most common functional assays measure platelet aggregation or platelet activation events (e.g., serotonin release assay; heparin-induced platelet activation (HIPA); flow cytometry). All assays have some sensitivity and specificity to HIT antibodies, but differ in terms of relative sensitivity and specificity for pathological HIT, as well as false negative and false positive error rate. This brief article overviews the different available laboratory methods, as well as providing a suggested approach to diagnosis or exclusion of HIT.

show abstract

Platelet microparticle generation assay for heparin-induced thrombocytopenia diagnosis: How should we express the results?

Cited by 6 publications

References 21 publications

Activated Platelet‐Derived and Leukocyte‐Derived Circulating Microparticles and the Risk of Thrombosis in Heparin‐Induced Thrombocytopenia: A Role for PF4‐Bearing Microparticles?

Activated Platelet‐Derived and Leukocyte‐Derived Circulating Microparticles and the Risk of Thrombosis in Heparin‐Induced Thrombocytopenia: A Role for PF4‐Bearing Microparticles?

Functional Assays in the Diagnosis of Heparin-Induced Thrombocytopenia: A Review

Laboratory tests for identification or exclusion of heparin induced thrombocytopenia: HIT or miss?

Contact Info

Product

Resources

About