Platelet mitochondrial bioenergetic analysis in patients with nephropathies and non-communicable diseases: a new method

Gvozdjáková, A; Sumbalová, Zuzana; Kucharská, J; Chladekova, A; Rausová, Zuzana; Vančová, Oľga; Komlosi, M.; Uličná, O; Mojto, Viliam

doi:10.4149/bll_2019_104

Cited by 18 publications

(20 citation statements)

References 25 publications

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“…Increased levels of total antioxidant capacity in diabetic hemodialyzed patients after CoQ 10 supplementation were documented (41). In agreement with others, we suppose that CoQ 10 supplementation could be important for improving the kidney function, and that clinical monitoring of platelet mitochondrial function, and plasma and platelet CoQ 10 levels could be important for determining the dosage of CoQ 10 supplementation (42,43,44,45,46,47).…”

Section: Plasma Coq 10-total In Subgroups Of Ckd Patients With Concomsupporting

confidence: 90%

The importance of coenzyme Q10 and its ratio to cholesterol in the progress of chronic kidney diseases linked to non- -communicable diseases

Gvozdjáková¹,

Kucharská²,

Sumbalová³

et al. 2020

BLL

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OBJECTIVES: The mortality of patients with chronic kidney diseases (CKD) increases with the decrease in glomerular fi ltration rate (eGFR). In the progress of CKD that is closely linked to non-communicable diseases (NCDs), the role of coenzyme Q 10 (CoQ 10) is not fully evaluated. We aimed to evaluate the importance of CoQ 10, CoQ 10 /cholesterol ratio, and oxidative stress in the progress of CKD. PATIENTS AND METHODS: The control group was constituted of 19 healthy subjects who volunteered to enrol in the study, CKD group consisted of 58 patients with CKD, of whom 54 had CKD combined with hypertension, 22 had CKD combined with hypertension and diabetes type 2 , and 18 had CKD combined with hypertension and statin therapy. We observed age, BMI, creatinine, uric acid, eGFR, hemoglobin, CRP, glucose, lipids fraction, and liver enzymes. Coenzyme Q 10-TOTAL (ubiquinol+ubiquinone) in platelets and plasma were determined using HPLC method with UV detection. Indexed of CoQ 10 /lipid fractions were evaluated. Oxidative stress was determined as thiobarbituric acid-reactive substances (TBARS). RESULTS: With increased stages of CKD, eGFR and CoQ 10 as well as its ratio to lipids were signifi cantly reduced while TBARS increased. CONCLUSION: We assume that lower endogenous CoQ 10 level may be one of the reasons of kidney dysfunction. CoQ 10 /lipids ratio and increase in oxidative stress can predict the progression of CKD in patients with arterial hypertension, diabetes mellitus and dyslipidemia (Tab. 2, Fig. 4, Ref. 49). Text in PDF www.elis.sk KEY WORDS: chronic kidney disease, non-communicable diseases, glomerular fi ltration, creatinine, coenzyme Q 10, oxidative stress, index of CoQ 10-TOTAL /lipids.

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Section: Plasma Coq 10-total In Subgroups Of Ckd Patients With Concomsupporting

confidence: 90%

The importance of coenzyme Q10 and its ratio to cholesterol in the progress of chronic kidney diseases linked to non- -communicable diseases

Gvozdjáková¹,

Kucharská²,

Sumbalová³

et al. 2020

BLL

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“…Increased respiration in intact platelets in patients after fluvastatin and atorvastatin treatment, in patients with diabetes mellitus, and in dialyzed patients was found [39]. Opposite results-decreased respiration in intact platelets-were reported in depressive patients [18].…”

Section: Discussionmentioning

confidence: 93%

“…Basal mitochondrial oxygen consumption in permeabilized platelets with Complex I-linked substrates (step 1PM) in CKD-ALL patients was slightly decreased vs. the control group (Figure 2). A previous study showed damaged CI-linked respiration in a patient after renal transplantation and strongly impaired CI-linked respiration in a patient with thrombocytopenia [39]. Decreased Complex I-linked respiration in permeabilized platelets in humans after statins treatment has been reported, suggesting the use of the respirometric analysis of mitochondrial function in platelets for studying changes in cellular energy metabolism in patients treated with statins [30].…”

Section: Discussionmentioning

confidence: 99%

Platelet Mitochondrial Respiration, Endogenous Coenzyme Q10 and Oxidative Stress in Patients with Chronic Kidney Disease

et al. 2020

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Chronic kidney disease (CKD) is characterized by a progressive loss of renal function and a decrease of glomerular filtration rate. Reduced mitochondrial function, coenzyme Q10 (CoQ10), and increased oxidative stress in patients with CKD contribute to the disease progression. We tested whether CoQ10 levels, oxidative stress and platelet mitochondrial bioenergetic function differ between groups of CKD patients. Methods: Twenty-seven CKD patients were enrolled in this trial, 17 patients had arterial hypertension (AH) and 10 patients had arterial hypertension and diabetes mellitus (AH and DM). The control group consisted of 12 volunteers. A high-resolution respirometry (HRR) method was used for the analysis of mitochondrial bioenergetics in platelets, and an HPLC method with UV detection was used for CoQ10 determination in platelets, blood, and plasma. Oxidative stress was determined as thiobarbituric acid reactive substances (TBARS). Results: Platelets mitochondrial respiration showed slight, not significant differences between the groups of CKD patients and control subjects. The oxygen consumption by intact platelets positively correlated with the concentration of CoQ10 in the platelets of CKD patients. Conclusion: A decreased concentration of CoQ10 and oxidative stress could contribute to the progression of renal dysfunction in CKD patients. The parameters of platelet respiration assessed by high-resolution respirometry can be used only as a weak biological marker for mitochondrial diagnosis and therapy monitoring in CKD patients.

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“…Hence, metabolically highly active but homogenous PLTs might be a valuable surrogate to investigate systemic changes of mitochondrial function, since they are involved in a variety of systemic reactions (e.g., hemostasis, immunoregulation, inflammation or oxidative stress) [18][19][20]. Assessing PLT bioenergetics as a systemic marker has been performed in the context of various diseases [15,19,[21][22][23][24][25][26][27][28] as well as training studies [29][30][31][32][33]. However, changes in PLT bioenergetics after an ultramarathon competition in the context of a field study has not been assessed so far.…”

Section: Introductionmentioning

confidence: 99%

Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies

Hoppel

Garcia-Souza

Kantner-Rumplmair

et al. 2021

Cells

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Human blood cells may offer a minimally invasive strategy to study systemic alterations of mitochondrial function. Here we tested the reliability of a protocol designed to study mitochondrial respiratory control in human platelets (PLTs) in field studies, using high-resolution respirometry (HRR). Several factors may trigger PLT aggregation during the assay, altering the homogeneity of the cell suspension and distorting the number of cells added to the two chambers (A, B) of the Oroboros Oxygraph-2k (O2k). Thus, inter-chamber variability (∆ab) was calculated by normalizing oxygen consumption to chamber volume (JO2) or to a specific respiratory control state (flux control ratio, FCR) as a reliable parameter of experimental quality. The method’s reliability was tested by comparing the ∆ab of laboratory-performed experiments (LAB, N = 9) to those of an ultramarathon field study (three sampling time-points: before competition (PRE, N = 7), immediately after (POST, N = 10) and 24 h after competition (REC; N = 10)). Our results show that ∆ab JO2 changed PRE-POST, but also for LAB-POST and LAB-REC, while all ∆ab FCR remained unchanged. Thus, we conclude that our method is reliable for assessing PLT mitochondrial function in LAB and field studies and after systemic stress conditions.

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Platelet mitochondrial bioenergetic analysis in patients with nephropathies and non-communicable diseases: a new method

Cited by 18 publications

References 25 publications

The importance of coenzyme Q10 and its ratio to cholesterol in the progress of chronic kidney diseases linked to non- -communicable diseases

The importance of coenzyme Q10 and its ratio to cholesterol in the progress of chronic kidney diseases linked to non- -communicable diseases

Platelet Mitochondrial Respiration, Endogenous Coenzyme Q10 and Oxidative Stress in Patients with Chronic Kidney Disease

Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies

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