Platelet recovery and survival measured in patients by quantitative polymerase chain reaction of mitochondrial DNA

Abstract

This RT-PCR method detects mt DNA polymorphisms in Caucasians for a highly sensitive and reproducible quantification of endogenous and allogeneic PLT numbers in blood samples from transfused patients with thrombocytopenia.

Quality Assessment of Established and Emerging Blood Components for Transfusion

“…Multiple groups have reported significantly lower hemolysis in units from premenopausal female donors when compared to donations from other donor populations [256, 261, 262, 265]. A number of theories have been suggested to explain this trend, based on known physiological differences between aging male and female blood donors and their circulating RBCs [261, 266, 267].…”

Measuring ADAMTS13 activity in patients with suspected thrombotic thrombocytopenic purpura: when, how, and why?

“…In the management of patients with suspected TTP, the clinician continues to rule. ADAMTS13 measurements certainly help, and the data or Wu, et al 2 tell us how ADAMTS13 measurements can be even more helpful. But even now, in 2015, management of patients with suspected TTP remains the responsibility of the clinician.…”

“…This question is addressed in this issue of Transfusion by Wu, et al 2 in their clear, concise, and clinically important study that begins with the statement, “clarification of the diagnostic and prognostic values of ADAMTS13 activity obtained during PE treatment is an unmet clinical need”. Their analysis of patients with acquired autoimmune TTP, whose diagnosis was supported by ADAMTS13 activity <10% plus ADAMTS13 inhibitor activity, documents that most patients (14 [78%] of 18) continue to have ADAMTS13 activity <10% even after three days of PEX.…”

“…The answer to this question is disappointing; different methods of measuring ADAMTS13 activity may yield different results. Wu, et al 2 used a unique mass spectrometry method that was developed in their laboratory and is not yet available elsewhere. The rest of us depend on a variety of commercial laboratories.…”

Mitochondrial DNA multiplex real‐time polymerase chain reaction inhibition assay for quality control of pathogen inactivation by ultraviolet C light in platelet concentrates

“…Quantification of mtDNA was performed with a real‐time PCR method as previously described . A commercial mtDNA standard (human HL‐60 DNA, standard reference material 2392‐1, National Institute of Standards & Technology) served as the reference for preparation of the standard curve for mtDNA quantification.…”