Platelet release reaction during EDTA-induced platelet agglutinations and inhibition of EDTA-induced platelet agglutination by anti-glycoprotein IIb/IIIa complex monoclonal antibody

Ryo, Ryukichi; Sugano, Wataru; Goto, M.; Takada, Moriatsu; Saigo, Katsuyasu; Hashimoto, Makoto; Yamaguchi, Nobuo

doi:10.1016/0049-3848(94)90114-7

Cited by 20 publications

(17 citation statements)

References 8 publications

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“…***P < 0·001, **P < 0·01, *P < 0·05 versus untreated samples. the findings that the anti-GP IIb/IIIa antibodies induce EDTA-PTCP (Casonato et al, 1994;Christopoulos & Machin, 1994;Ryo et al, 1994). In addition, EDTA has been demonstrated to prevent the aggregation of platelets from normal donors by increasing platelet surface charge .…”

Section: Discussionmentioning

confidence: 89%

Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA‐dependent pseudothrombocytopenia

et al. 1997

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Summary.Although EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) is of practical importance because failure to recognize this clinical entity may result in misdiagnosis and subsequent mismanagement of the patients, the pathophysiological nature of EDTA-PTCP remains unknown. To develop an effective way to evaluate the platelet counts in patients with EDTA-PTCP, we introduced aminoglycosidessupplemented anticoagulating agents. When kanamycin was pre-supplemented with EDTA for anticoagulating blood samples from EDTA-PTCP patients there was no significant change in the platelet counts and the morphology of blood cells after 150 min of incubation at room temperature. Furthermore, when kanamycin was added to EDTA-anticoagulated blood samples from EDTA-PTCP patients within 30 min after blood withdrawal, rapid dissociation of platelets without apparent morphological changes of blood cells was observed, and complete blood cell counts as well as the histogram patterns were almost the same as those examined immediately after blood sampling. The dissociation of aggregated platelets was also detected when other antibiotics were used, although it was associated with some extent of morphological changes of blood cells. These findings indicate that the supplementation of aminoglycosides either before or after blood sampling is a useful method for the diagnosis EDTA-PTCP and for the evaluation of platelet counts in patients with EDTA-PTCP.

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Section: Discussionmentioning

confidence: 89%

Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA‐dependent pseudothrombocytopenia

et al. 1997

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“…5) and by immunocytochemical labeling [87], which demonstrates the differential distribution of various substances within the nucleoid and two zones within the matrix. This compartmentalization may be the reason for the differential release of a-granular substances that is observed after in vitro stimulation of platelets [35,79].…”

Section: Storage Granulesmentioning

confidence: 99%

The storage lesion of platelets: ultrastructural and functional aspects

Klinger

1996

Annals of Hematology

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When prepared and stored as concentrates, platelets undergo a lot of structural, biochemical and functional alterations that lead to an impaired function after transfusion. Besides signs of activation like disc-to-sphere transformation, extension of pseudopodes and loss of storage granules, platelets may display a swollen open canalicular system and changes in the structure of their alpha-granules. These partly reversible morphological alterations correspond to a deterioration of basic metabolic parameters and a decrease in the reactivity of stored platelets to weak agonists. All these changes occur to a very different degree depending on the methods of preparation and storage. With the introduction of acetate-containing additive solutions, the storage conditions could be greatly improved, and platelets from pooled buffy coats and stored in an acetate-containing medium with at least 20% autologous plasma show the best structural integrity over 8 days of storage.

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“…are found most frequently in EDTA-dependent pseudothrombocytopenia; however, combinations of IgG and IgA, IgM alone, and cold reactive IgA also have been described (8,(26)(27)(28)(29)(30). It has been shown that the antigenic determinant of the EDTA-dependent antibodies may be the GP Ilb/IIIa complex (29).…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that the antigenic determinant of the EDTA-dependent antibodies may be the GP Ilb/IIIa complex (29). EDTA-dependent pseudothrombocyotpenia is most frequently observed in patients with prolonged severe illnesses (8, 3 1), and is frequently associated with autoimmune, neoplastic, or liver diseases (8).…”

Section: Discussionmentioning

confidence: 99%

Guillain-Barre Syndrome and Ethylene Diamine Tetraacetic Acid-Dependent Pseudothrombocytopenia Associated with Mumps.

Sawazaki¹,

Nakamura²,

Jyokaji³

et al. 1996

Intern. Med.

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A 35-year-old man with Guillain-Barre syndrome and ethylene diamine tetraacetic acid (EDTA)-dependent pseudothrombocytopenia associated with serologically-confirmed mumpsis presented. A polyneuropathy developed 18 days after the onset of mumps, that improved with plasmapheresis. A decreased platelet count was observed 25 days after the onset of mumps attributed to platelet agglutination in blood anticoagulated with EDTA,and the agglutination was prevented by other anticoagulants. Guillain-Barre syndromeassociated with mumpsis rare and EDTA-dependent pseudothrombocytopenia following mumpshas never been documented. This represents the first report of Guillain-Barre syndrome and EDTA-dependentpseudothrombocytopenia following mumps.Furthermore, our findings suggest activation of the humoral immune response as a potential pathogenesis. (Internal Medicine 35: 996-999, 1996)

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Platelet release reaction during EDTA-induced platelet agglutinations and inhibition of EDTA-induced platelet agglutination by anti-glycoprotein IIb/IIIa complex monoclonal antibody

Cited by 20 publications

References 8 publications

Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA‐dependent pseudothrombocytopenia

Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA‐dependent pseudothrombocytopenia

The storage lesion of platelets: ultrastructural and functional aspects

Guillain-Barre Syndrome and Ethylene Diamine Tetraacetic Acid-Dependent Pseudothrombocytopenia Associated with Mumps.

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