Platelet Rich Plasma Preparation Protocols: A Preliminary Study

Nugraha, Hans Kristian; Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Jusak

doi:10.20473/ijtid.v3i2.216

Cited by 11 publications

(13 citation statements)

References 16 publications

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“…Among PRP produced from three different groups of intervention (centrifugation speed and duration of centrifugation), the highest platelet rise was found in group 2, with an increase in platelets counts as of 3.69 times compared to the baseline. This is in line with the studies of Hans et al as cited Nugraha et al, 7 showing the highest increase in platelets with speed and duration similar to the intervention conducted in this study, namely 1300 RCF for the first 5 minutes of centrifugation and 2300 RCF for 7 minutes in the second centrifugation. Platelet increase in the studies of Hans et al was 4.11 times higher that the initial platelets.…”

Section: Discussionsupporting

confidence: 93%

“…The study results found that the highest platelet increase was obtained at a speed of 1300 RCF for 5 minutes at the first centrifugation and a speed of 2300 for 7 minutes with a platelet increase of 4.11 times. 7 The use of anticoagulants is also a very important factor in maintaining the function, integrity, and morphology of platelets. Some researchers argue that the use of Ethylenediaminetetraacetic Acid (EDTA) anticoagulants can damage platelet membranes, and recommend the use of Acid Citrate Dextrose (ACD).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Platelet Counts and Platelet-Derived Growth Factor-Bb Levels in Platelet Rich Plasma Produced with Edta as Anticoagulant in Three Different Centrifugation Methods

2020

IJPHRD

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Background: Platelet rich plasma (PRP) is an autologous product made of whole blood through centrifugation process producing high platelet concentrate in a small volume of plasma. This high platelet concentrate can lead to high growth factor levels that play important roles in the nature of thrombosis, hemostasis and wound healing. PRP has been widely used in clinical setting, however standardized procedure of PRP production has been lacking, mainly the procedure related to duration and speed of centrifugation, and also anticoagulant used. Aim:To analyze the difference between platelet counts and PDGF-BB levels in PRP produced from various preparation methods (centrifugation speed and duration), using Ethylenediaminetetraacetic Acid /EDTA as anticoagulant.Method: This study used experimental laboratory design, involving 34 healthy volunteers that met inclusion criteria. These subjects were divided into three groups with different centrifugation methods that adopted from previous study. Results:The platelet counts was found in group 1, 2, and 3 with an increase of, 2,69, 3,69, and 2,53 times from the initial platelet counts respectively and there was significant difference between platelet counts before and after treatment on three groups that used EDTA (p=0,000, p=0,003, and p=0,002 for group 1, 2 and 3 respectively). PDGF-BB levels in this group was also higher than the remaining groups. Conclusion:The highest platelet counts and PDGF-BB levels was found in PRP with EDTA produced from first spin at 2800 RPM in five minutes, and second spin at 3800 RPM in seven minutes. This study suggests further research on qualitative assessments of platelets and PDGF-BB resulted from various preparation methods (centrifugation and anticoagulant usage).

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Analysis of Platelet Counts and Platelet-Derived Growth Factor-Bb Levels in Platelet Rich Plasma Produced with Edta as Anticoagulant in Three Different Centrifugation Methods

2020

IJPHRD

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“…Researchers have reported that PRP has four to 6 the normal level of growth factors, which results in fibrocytes relocation and enlistment of neovascular development [14] in particular, in an ongoing investigation of one study [15] a beneficial outcome of injection of PRP in the normal extensor root for sidelong epicondylitis was seen. It exhibits that a solitary infusion of PRP enhances pain and capacity more than corticosteroid infusion.…”

Section: Discussionmentioning

confidence: 99%

“…The utilization of autologous PRP is certifiably not another treatment. The healing cascade which is the physiological reaction to any damage or careful intercession, is very much reported and depends on proteins that are conveyed to the mending site by platelets and white blood cells notwithstanding those proteins that are available in the plasma [14]. Effective tissue recuperating and recovery requires a framework or lattice, undifferentiated cells and flag proteins and bond particles (development factors).…”

Section: Discussionmentioning

confidence: 99%

Platelet Rich Plasma versus Corticosteroid Local Injection Results in Treatment of Planter Fasciitis in New-Cairo

Akl¹,

Sadek²,

Dayem³

et al. 2019

Zagazig University Medical Journal

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Background: Chronic plantar fasciitis is a common orthopedic condition that can demonstrate hard to effectively treat. In this study, platelet-rich plasma (PRP), a concentrated bioactive blood segment wealthy in cytokines and growth factors, was compared to cortisone injection in the treatment of Chronic plantar fasciitis resistant to traditional nonoperative management. Methods: Between October 2016 and the end of September 2017; sixty patients exhibited to New Cairo clinic with chronic plantar fasciitis, 30 patients were treated with PRP infusion, and 30 patients were treated with corticosteroid injection. This study compares the efficacy of (PRP) with that of corticosteroid local injection in chronic plantar fasciitis toward the finish of follow up time of 6 months concurring AOFAS score framework out of 100. Results: At the end of follow up, group treated by plasma rich platelets injection demonstrated that mean score was 83.5 of 100. While other treated by corticosteroid injection demonstrated mean score was 61.8 of 100 .No complication detected after the end of follow up period. Conclusions: Both PRP injection and corticosteroid injection are modalities for treatment of chronic plantar fasciitis. PRP preparation is a costly and complicated procedure than corticosteroid injection but PRP is more powerful and strong than cortisone injection for the treatment of chronic plantar fasciitis. Concerning corticosteroid side effects PRP infusion is safer in treatment of Chronic plantar fasciitis.

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“…Recentemente, estudos mostram benefícios do plasma rico em plaquetas em diversas áreas da medicina como, por exemplo, no tratamento de problemas musculoesquelético e articular, vantajoso para atletas profissionais, como jogadores de futebol, que precisam da rápida cicatrização para retornar à atividade. (4,5) A doutrina nacional e estrangeira tem destacado as propriedades regenerativas do PRP, sendo considerado um agente catalisador no processo de reparo tecidual. O processamento do PRP envolve a separação das plaquetas com todas as suas propriedades, podendo, assim, ser considerado uma fonte autógena de fatores de crescimento, pois trata-se de princípio terapêutico inovador, acelerando as etapas de reparo da ferida.…”

Section: Introductionunclassified