Platelet storage in more than 90% additive solution containing glucose and bicarbonate has the potential to increase shelf life

Bashir, S.; Kemsley, Kelly; Min, Kyungyoon; Swann, Ian D.; Cardigan, Rebecca

doi:10.1111/trf.14962

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…Platelets resuspended in PAS‐E are also preserved satisfactorily in comparison to those resuspended in PAS‐B 87,88 . In parallel, the use of PAS‐E and PAS‐G (M‐Sol) maintained platelet metabolic activity, as evidenced by PH and lactate 89 . PAS containing glucose, magnesium and potassium also exhibit attractive advantages in other experiments 90,91 .…”

Section: Pas Cold and Frozen Platelets–reconsidered Conditionsmentioning

confidence: 95%

“… 87 , 88 In parallel, the use of PAS‐E and PAS‐G (M‐Sol) maintained platelet metabolic activity, as evidenced by PH and lactate. 89 PAS containing glucose, magnesium and potassium also exhibit attractive advantages in other experiments. 90 , 91 Increased PMP levels in PAS tend to bind to vWF, 92 and washing by another PAS, bicarbonate Ringer's solution supplemented with acid‐citrate‐dextrose Formula A (BRS‐A) used in Japan, can reduce PMPs, sCD40L and RANTES levels.…”

Section: Pas Cold and Frozen Platelets–reconsidered Conditionsmentioning

confidence: 99%

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The platelet storage lesion, what are we working for?

Liu,

Su,

Guo

et al. 2023

Clinical Laboratory Analysis

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BackgroundPlatelet concentrate (PC) transfusions are crucial in prevention and treatment of bleeding in infection, surgery, leukemia, and thrombocytopenia patients. Although the technology for platelet preparation and storage has evolved over the decades, there are still challenges in the demand for platelets in blood banks because the platelet shelf life is limited to 5 days due to bacterial contamination and platelet storage lesions (PSLs) at 20–24°C under constant horizontal agitation. In addition, the relations between some adverse effects of platelet transfusions and PSLs have also been considered. Therefore, understanding the mechanisms of PSLs is conducive to obtaining high quality platelets and facilitating safe and effective platelet transfusions.ObjectiveThis review summarizes developments in mechanistic research of PSLs and their relationship with clinical practice, providing insights for future research.MethodsAuthors conducted a search on PubMed and Web of Science using the professional terms “PSL” and “platelet transfusion.” The obtained literature was then roughly categorized based on their research content. Similar studies were grouped into the same sections, and further searches were conducted based on the keywords of each section.ResultsDifferent studies have explored PSLs from various perspectives, including changes in platelet morphology, surface molecules, biological response modifiers (BMRs), metabolism, and proteins and RNA, in an attempt to monitor PSLs and identify intervention targets that could alleviate PSLs. Moreover, novel platelet storage conditions, including platelet additive solutions (PAS) and reconsidered cold storage methods, are explored. There are two approaches to obtaining high‐quality platelets. One approach simulates the in vivo environment to maintain platelet activity, while the other keeps platelets at a low activity level in vitro under low temperatures.ConclusionUnderstanding PSLs helps us identify good intervention targets and assess the therapeutic effects of different PSLs stages for different patients.

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Section: Pas Cold and Frozen Platelets–reconsidered Conditionsmentioning

confidence: 95%

Section: Pas Cold and Frozen Platelets–reconsidered Conditionsmentioning

confidence: 99%

The platelet storage lesion, what are we working for?

Liu,

Su,

Guo

et al. 2023

Clinical Laboratory Analysis

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“…Conflicting evidence has recently emerged suggesting that extremely low levels of glucose in PAS with reduced plasma carryover would be associated with the development of a proapoptotic phenotype in concentrates stored beyond 7 days . Newer PAS formulations containing glucose may be beneficial by providing necessary metabolic substrates and better buffering to maintain pH . Cold storage and cryopreservation procedures differentially affect platelet metabolism and function when compared with conventional platelet storage conditions …”

Section: In Vitro Testsmentioning

confidence: 99%

Platelet in vitro assays: their correspondence with their in vivo hemostatic potential

Escolar

McCullough

2019

Transfusion

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Developments during the past few years have resulted in multiple kinds of platelet products for transfusion. This involves different collection methods, containers, preservative solutions, modifications of storage temperatures and durations, and additional treatments such as pathogen reduction. Much experience has been obtained testing these processes in vitro to seek indications of in vivo effectiveness. Availability of an in vitro method that correlated with in vivo effectiveness would be extremely valuable for these different kinds of platelet products and as more innovation in platelet preparation occurs in the future. This report reviews the methods for in vitro platelet testing with a view to their in vivo implications and whether such testing could be helpful in projecting the clinical effectiveness of different platelet products. ABBREVIATIONS: ADP = adenosine diphosphate; ATP = adenosine triphosphate; GP = glycoprotein; PAR = proteaseactivated receptor; PASs = platelet additive solutions; PCs = platelet concentrates; PRT = pathogen reduction technology; TEG = thromboelastography; TRAP = thrombin-activated receptor peptide; VWF = von Willebrand factor. From the

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“…To reduce ATRs, the clinical application of platelet-additivesolution suspended PC (PAS-PC) was first launched in 1991 in Sweden, and second-to fifth-generation PAS components for improving the quality of PC during storage were developed [4][5][6][7]. Because of the reduction in the plasma component ($35% of the original plasma) in PAS-PCs, the frequency of PC-related ATRs can be significantly decreased to about half of that in non-treated PCs [8,9].…”

Section: Introductionmentioning

confidence: 99%

Dual preparation of plasma and platelet concentrates in platelet additive solution from platelet concentrates in plasma using a novel filtration system

Hayashi

Fujimura

et al. 2021

Vox Sanguinis

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Background and Objectives: Platelet concentrates suspended in a platelet additive solution (PAS-PC) are associated with a reduction in allergic response and are suitable for preparing pathogen-inactivated PC. We aimed to develop an efficient platform for the dual preparation of PAS-PC and platelet-poor plasma.Materials and Methods: PAS-PC was prepared in six steps by using a hollow-fibre system based on cross-flow filtration: priming, loading PC, loading PAS, collection of filtered liquid (flow-through) and collection of platelets by washing with PAS followed by washing with air. In this study, the efficacy of platelet and plasma protein recovery and characteristics of recovered PAS-PC and flow-through plasma were analysed in detail.Results: Recoveries of platelet in PAS-PC and plasma protein in the flow-through were 95.4% AE 3.7% and 61.6% AE 5.0%, respectively. The residual plasma protein in PAS-PC was 34.1% AE 2.8%. Although the expression level of CD62P, a platelet activation marker, in recovered platelets was approximately 1.2-fold of that in original platelets, swirling patterns were well retained, and aggregation in PAS-PC was not visible. Agonist-induced aggregabilities, platelet morphology and hypotonic shock recovery were conserved. The patterns of plasma protein and lipoprotein in the flow-through were comparable with those in the original PCs. The multimeric pattern analysis of VWF remained unaltered. Conclusion:We propose a highly efficient preparation system that enables the simultaneous production of PAS-PC and platelet-poor plasma. It also achieves a high recovery of functionally well-retained platelets with very low activation.

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Platelet storage in more than 90% additive solution containing glucose and bicarbonate has the potential to increase shelf life

Cited by 5 publications

References 23 publications

The platelet storage lesion, what are we working for?

The platelet storage lesion, what are we working for?

Platelet in vitro assays: their correspondence with their in vivo hemostatic potential

Dual preparation of plasma and platelet concentrates in platelet additive solution from platelet concentrates in plasma using a novel filtration system

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