Platelets redox balance assessment: Current evidence and methodological considerations

Komosa, Anna; Rzymski, Piotr; Perek, Bartłomiej; Ropacka-Lesiak, Mariola; Lesiak, Maciej; Siller‐Matula, Jolanta M.; Poniedziałek, Barbara

doi:10.1016/j.vph.2017.06.002

Cited by 25 publications

(31 citation statements)

References 111 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The significantly greater Fe content found in supplements aimed to support pregnancy compared to the other studied groups can be explained by the increased demand of this element in pregnant women due to excessive loss of blood and risk of anemia (Milman et al 1999). In other cases, its increased intake can lead to Fe overloading associated with gastrointestinal distress (Frykman et al 1994) and the generation of reactive oxygen species through Fenton and Haber-Weiß reactions and subsequent oxidative stress (Galaris and Pantopoulos 2008;Komosa et al 2017). However, it should be highlighted that the significantly exceeded GL values of Fe in other investigated supplement groups were found sporadically.…”

Section: Discussionmentioning

confidence: 99%

Monitoring of essential and toxic elements in multi-ingredient food supplements produced in European Union

Poniedziałek

Niedzielski

Kozak³

et al. 2018

J Consum Prot Food Saf

Self Cite

View full text Add to dashboard Cite

The market of food supplements (FS) in European Union (EU) is on the rise. The present study investigated the content of essential (Ca, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Zn) and toxic elements (Cd, Cr(VI), Ni and Pb) in 168 FS produced in the EU and designed to support various health aspects (nutrition, neurological function, reproduction, weight loss, cosmetic appearance). The majority of FS did not exceed a dissolution time of 60 min in hydrochloric acid (89.8%) and phosphate buffer (85.7%). Cd and Cr(VI) were not detected in any FS, Ni and Pb were present sporadically (in 10.1 and 6.5%, respectively) at mean concentrations of 0.06 and 0.07 mg/single unit dose, respectively. All FS containing detectable Pb levels had exceeded a maximum allowance level (3.0 mg kg -1 ) set by the European Commission. Generally, the determined concentrations of essential elements fall greatly below recommended safety upper limits, except Fe in selected FS (mainly for pregnant women). Compared to the value declared on the product label, the majority of tested FS displayed mineral content below the accepted margin (70-130%). In general, the majority of the studied FS can be considered safe although discrepancies between declared and determined mineral content are worrisome. These findings are important in view of FS consumer safety.

show abstract

Section: Discussionmentioning

confidence: 99%

Monitoring of essential and toxic elements in multi-ingredient food supplements produced in European Union

Poniedziałek

Niedzielski

Kozak³

et al. 2018

J Consum Prot Food Saf

Self Cite

View full text Add to dashboard Cite

show abstract

“…The collective data support the prediction that PRF preparations using silica-coated tubes could be toxic to the surrounding cells at implantation sites. During and after preparation of PRF matrix, silica microparticles may also over-activate or disrupt platelets [18] and other blood cells in the PRF matrix to reduce its therapeutic potency and efficacy.…”

Section: Discussionmentioning

confidence: 99%

Acute cytotoxic effects of silica microparticles used for coating of plastic blood-collection tubes on human periosteal cells

et al. 2020

View full text Add to dashboard Cite

Because of its simple operation, platelet-rich fibrin (PRF) is becoming more popular than the original form, platelet-rich plasma (PRP), in regenerative dentistry. PRF preparation requires plain glass blood-collection tubes, but not either anticoagulants or coagulation factors. However, such glass tubes designed for laboratory testing are no longer commercially available. Although several glass tubes specifically designed for PRF preparation are available, many clinicians prefer to obtain stably supplied substitutes, such as silica-coated plastic tubes produced by major medical device companies. The quality of PRF prepared by silica-coated tubes has not been assessed and we previously reported significant contamination of silica microparticles in the resulting PRF matrix and alerted clinicians against the use for PRF preparation. To further assess the biosafety of the silica microparticles, we presently examined their effects on human normal periosteal cells derived from alveolar bone. The periosteal cells were obtained from explant cultures of small periosteal tissues obtained from healthy donors. Silica microparticles were obtained from silica-coated tubes and added to cell cultures. Cellular responses were monitored using a tetrazolium assay, phase-contract inverted microscopy, an immunofluorescence method, and scanning electron microscopy. Silica microparticles adsorbed onto the cell surface with seemingly high affinity and induced apoptosis, resulting in significant reduction of cell proliferation and viability. These findings suggest that silica microparticles contained in plastic tubes for the purpose of blood coagulation are hazardous for various cell types around sites where silicacontaminated PRF matrices are implanted.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

show abstract

“…Importantly, the antioxidant capacity of the studied mushroom extracts remained in a positive association with the rate at which they diminished the AA-induced aggregation of platelets. It is now well established that reactive oxygen species play a significant role in the platelet activation cascade and that antioxidants such as resveratrol and Trolox can inhibit platelet responses to various stimulating events [20,65]. As shown previously, the hot water extracts of P. eryngii cultivated on substrates enriched with selected trace elements reveal increased antioxidative activities in isolated human platelets by preventing ROS formation and lipid peroxidation [19], which opens an interesting arena in which to test whether such an approach may also potentiate the anti-aggregative properties of this mushroom.…”

Section: Discussionmentioning

confidence: 99%

“…A methanol extract of Pleurotus florida inhibited adenosine-5 diphosphate-induced aggregation in isolated human platelets [17] while a dry extract of Stereum hirsutum diminished platelet activation by blocking the thrombin active site [18]. Recently, aqueous extracts of Pleurotus mushrooms have been demonstrated to successfully prevent an increase in platelet's concentration of reactive oxygen species (ROS), which are known to mediate platelet activation via different pathways [19,20]. Altogether, these findings highlight the need to further explore and compare the potential antiplatelet activities exerted by different mushrooms.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Mushroom Extracts on Human Platelet and Blood Coagulation: In vitro Screening of Eight Edible Species

et al. 2019

Self Cite

View full text Add to dashboard Cite

Cardiovascular diseases remain the leading global cause of mortality indicating the need to identify all possible factors reducing primary and secondary risk. This study screened the in vitro antiplatelet and anticoagulant activities of hot water extracts of eight edible mushroom species (Agaricus bisporus, Auricularia auricularia-judae, Coprinus comatus, Ganoderma lucidum, Hericium erinaceus, Lentinula edodes, Pleurotus eryngii, and Pleurotus ostreatus) increasingly cultivated for human consumption, and compared them to those evoked by acetylsalicylic acid (ASA). The antioxidant capacity and concentration of polysaccharides, phenolic compounds, organic acids, ergosterol, macro elements, and trace elements were also characterized. The most promising antiplatelet effect was exhibited by A. auricularia-judae and P. eryngii extracts as demonstrated by the highest rate of inhibition of adenosine-5′-diphosphate (ADP)-induced and arachidonic acid (AA)-induced aggregation. The response to both extracts exceeded the one evoked by 140 µmol/L of ASA in the ADP test and was comparable to it in the case of the AA test. Such a dual effect was also observed for G. lucidum extract, even though it was proven to be cytotoxic in platelets and leukocytes. The extract of P. ostreatus revealed an additive effect on AA-induced platelet aggregation. None of the mushroom extracts altered the monitored coagulation parameters (prothrombin time, prothrombin ratio, and International Normalized Ratio). The effect of mushroom extracts on platelet function was positively related to their antioxidative properties and concentration of polysaccharides and ergosterol, and inversely related to zinc concentration. The study suggests that selected mushrooms may exert favorable antiplatelet effects, highlighting the need for further experimental and clinical research in this regard.

show abstract

Platelets redox balance assessment: Current evidence and methodological considerations

Cited by 25 publications

References 111 publications

Monitoring of essential and toxic elements in multi-ingredient food supplements produced in European Union

Monitoring of essential and toxic elements in multi-ingredient food supplements produced in European Union

Acute cytotoxic effects of silica microparticles used for coating of plastic blood-collection tubes on human periosteal cells

The Effect of Mushroom Extracts on Human Platelet and Blood Coagulation: In vitro Screening of Eight Edible Species

Contact Info

Product

Resources

About