Platelets release proinflammatory microparticles in anti-neutrophil cytoplasmic antibody-associated vasculitis

Miao, Di; Ma, Tiantian; Chen, Min; Zhao, Ming‐Hui

doi:10.1093/rheumatology/kez044

Cited by 22 publications

(18 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PMPs can bind to the membrane structure in the endothelial cells to activate these cells, which then recruit platelets into the injured endothelium (20). In addition, PMPs may also induce the expression of intercellular adhesion molecule 1 (ICAM1) and increase the expression of pro-inflammatory cytokines IL-1, IL-6 and IL-8 (21,22) which are consistent with our results. Barry et al (21) showed PMPs enhanced the adhesion of monocytes to endothelial cells, which might be associated with the arachidonic acid in the PMPs.…”

Section: Discussionsupporting

confidence: 90%

Platelet-derived microparticles promote phagocytosis of oxidized low-density lipoprotein by macrophages, potentially enhancing foam cell formation

Feng¹,

Chen²,

Fan³

et al. 2019

Ann. Transl. Med.

View full text Add to dashboard Cite

Background: The interaction between platelets and macrophages plays an important role in the development and progression of atherosclerosis (AS). This study aimed to investigate the role of platelet microparticles (PMPs) in the development of foam cells.Methods: PMPs are generated by activating platelets with thrombin and separated by ultracentrifugation.The macrophages were treated with PMPs, the phagocytosis of oxidized low-density lipoprotein (Ox-LDL) and formation of foam cells were evaluated by flow cytometry and confocal microscopy, respectively, and the inflammatory factors cytokines in the supernatant were detected by ELISA.

show abstract

Section: Discussionsupporting

confidence: 90%

Platelet-derived microparticles promote phagocytosis of oxidized low-density lipoprotein by macrophages, potentially enhancing foam cell formation

Feng¹,

Chen²,

Fan³

et al. 2019

Ann. Transl. Med.

View full text Add to dashboard Cite

show abstract

“…Platelets are first responders during vascular injury and they are inflammatory effector cells closely correlated with the activity of vasculitis (7, 174). In line with that PMPs containing proinflammatory cytokines are increased in AAVs patients and supposed to trigger an acute inflammatory state in AAVs (175). Figure 4 summarizes the platelet activation and subsequent effects in AAVs.…”

Section: Platelets Role In Skin Autoimmune Diseasessupporting

confidence: 58%

Platelets in Skin Autoimmune Diseases

2019

View full text Add to dashboard Cite

Systemic lupus erythematosus (SLE), systemic sclerosis (SSc), and small vessel vasculitis are three autoimmune diseases frequently manifested in the skin. They share common pathogenic features, including production of autoantibodies, loss of tolerance to self-antigens, tissue necrosis and fibrosis, vasculopathy and activation of the coagulation system. Platelets occupy a central part within the coagulation cascade and are well-recognized for their hemostatic role. However, recent cumulative evidence implicates their additional and multifaceted immunoregulatory functions. Platelets express immune receptors and they store growth factors, cytokines, and chemokines in their granules enabling a significant contribution to inflammation. A plethora of activating triggers such as damage associated molecular patterns (DAMPs) released from damaged endothelial cells, immune complexes, or complement effector molecules can mediate platelet activation. Activated platelets further foster an inflammatory environment and the crosstalk with the endothelium and leukocytes by the release of immunoactive molecules and microparticles. Further insight into the pathogenic implications of platelet activation will pave the way for new therapeutic strategies targeting autoimmune diseases. In this review, we discuss the inflammatory functions of platelets and their mechanistic contribution to the pathophysiology of SSc, ANCA associated small vessel vasculitis and other autoimmune diseases affecting the skin.

show abstract

“…It is well illustrated that the ANCA‐activated neutrophils, which exerted abnormal cytokine production accompanied by the release of reactive oxygen species, lytic enzymes, and neutrophil extracellular traps formation, is the crucial role of vascular endothelial injury in MPA 6 . Moreover, some components in blood, such as pro‐inflammatory cytokines, microparticles, and exosomes can also cause vascular endothelial injury, either directly or indirectly 7 …”

Section: Introductionmentioning

confidence: 99%

Plasma exosomal miRNAs involved in endothelial injury in microscopic polyangiitis patients

et al. 2020

View full text Add to dashboard Cite

Microscopic polyangiitis (MPA) is a systemic autoimmune disease that primarily affects the small and medium blood vessels. Endothelial injury is one of the pathological hallmarks of MPA. However, the pathogenesis for this has not yet been fully elucidated. Exosomal microRNAs (miRNAs) have recently emerged as a new molecular pattern involved in the endothelial injury in other diseases. Hence, we speculated that MPA plasma-derived exosomes (MPA-exo) could induce the endothelial injury, which was likely to be aroused by the dysregulated exosomal miRNAs in MPA. In the present study, plasma-derived exosomes were isolated and identified. MPA-exo could be internalized by human renal glomerular endothelial cells (HRGECs) in vitro and induced HRGECs injury. Subsequently, a series of differentially expressed miRNAs in MPAexo were identified by high-throughput sequencing analysis. Further bioinformatics analysis for the target genes of these differentially expressed miRNAs showed a potential mechanism for their possible role in MPA endothelial injury. Notably, we revealed a considerable correlation between miR-185-3p, miR-125a-3p, and clinical parameters. In conclusion, the current study revealed that differentially expressed miRNAs in MPA-exo are associated with the endothelial injury. Our results suggested that these miRNAs and their target genes might be involved in the inflammation process of MPA. K E Y W O R D Shigh-throughput sequencing, microRNA profile, plasma exosomes 6216 | WANG et Al.

show abstract

Platelets release proinflammatory microparticles in anti-neutrophil cytoplasmic antibody-associated vasculitis

Cited by 22 publications

References 45 publications

Platelet-derived microparticles promote phagocytosis of oxidized low-density lipoprotein by macrophages, potentially enhancing foam cell formation

Platelet-derived microparticles promote phagocytosis of oxidized low-density lipoprotein by macrophages, potentially enhancing foam cell formation

Platelets in Skin Autoimmune Diseases

Plasma exosomal miRNAs involved in endothelial injury in microscopic polyangiitis patients

Contact Info

Product

Resources

About