Platinum as a Novel Nanoparticle for Wound Healing Model in Drosophila melanogaster

Bag, Janmejaya; Mukherjee, Sumit; Tripathy, Manamohan; Mohanty, Rudramadhab; Shendha, Pranab Kumar; Hota, Garudadhwaj; Mishra, Monalisa

doi:10.1007/s10876-022-02292-9

Cited by 9 publications

(13 citation statements)

References 62 publications

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“…ROS formation reduced significantly in the nanocomposite-treated group compared to the control. Thus, the NBT (11383213001, Sigma-Aldrich, Merck, Germany) assay suggests that the MnFe 2 O 4 @poly( t BGE-alt-PA) nanocomposite plays a vital role in ROS scavenging , as shown in Figure . In control, the absorbance value at 595 nm was found to be 0.4731 ± 0.38.…”

Section: Resultsmentioning

confidence: 99%

“…Scanning electron microscopy (SEM) (JEOL JSM-6480LV) analysis followed by coating the samples with platinum. The quantity of manganese and iron was determined by energy-dispersive spectroscopy (EDS) analysis. , …”

Section: Methodsmentioning

confidence: 99%

“…The quantity of manganese and iron was determined by energydispersive spectroscopy (EDS) analysis. 40,41 2.2.12. Phenotype Observation.…”

Section: Introductionmentioning

confidence: 99%

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In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Kumar

Bauri

Sahu

et al. 2023

ACS Appl. Bio Mater.

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Nanocomposites have significantly contributed to biomedical science due to less aggregation behavior and enhanced physicochemical properties. This study synthesized a MnFe2O4@poly(tBGE-alt-PA) nanocomposite for the first time and physicochemically characterized it. The obtained hybrid nanomaterial was tested in vivo for its toxicological properties before use in drug delivery, tissue engineering fields, and environmental applications. The composite was biocompatible with mouse fibroblast cells and hemocompatible with 2% RBC suspension. This nanocomposite was tested on Drosophila melanogaster due to its small size, well-sequenced genome, and low cost of testing. The larvae’s crawling speed and direction were measured after feeding. No abnormal path and altered crawling pattern indicated the nonappearance of abnormal neurological disorder in the larva. The gut organ toxicity was further analyzed using DAPI and DCFH-DA dye to examine the structural anomalies. No apoptosis and necrosis were observed in the gut of the fruit fly. Next, adult flies were examined for phenotypic anomalies after their pupal phases emerged. No defects in the phenotypes, including the eye, wings, abdomen, and bristles, were found in our study. Based on these observations, the MnFe2O4@poly(tBGE-alt-PA) composite may be used for various biomedical and environmental applications.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Kumar

Bauri

Sahu

et al. 2023

ACS Appl. Bio Mater.

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“…The ies treated with C-PVP@PVA membrane took more than 3 hours to heal the wound, whereas C-PVP-Au@PVA treated ies healed within 3 hours and in some cases, lesser than that. Although the control ies took much more time than both treated ies, it was nearly about 6 hours (Bag et al 2022). The ow of time ies from the control group showed a worse condition.…”

Section: Wound Applicationsmentioning

confidence: 90%

Polymeric curcumin nanospheres for lysozyme aggregation inhibition, antibacterial and wound healing applications

Rananaware

Bauri

Keri

et al. 2022

Preprint

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Curcumin is a renowned drug for its numerous applications as an anti-inflammatory, anti-oxidant, anti-allergic, and anti-hyperglycemic agent. However, its clinical applications are limited because of its low physicochemical stability, solubility, cellular uptake, and rapid systemic clearance. Various nanoformulations have been proposed and characterized to upsurge curcumin's solubility and bioavailability. Nanomaterials have much potential to modulate the aggregation kinetics of several proteins, which may lead to the treatment option for several neurological disorders and act as a multi-therapeutic agent. The present study reports highly stable polymeric nanoparticles consisting of curcumin and polyvinylpyrrolidone, C-PVP, and further conjugated with gold nanoparticles, C-PVP-Au, were synthesized. The synthesized conjugates C-PVP and C-PVP-Au were investigated for amyloid aggregation inhibition activity, antimicrobial activity, and wound healing applications. The anti-amyloidogenic capacity of nanoconjugates for model protein, hen egg-white lysozyme (HEWL), was examined at pH and temperature-induced aggregation. The ThT binding assay, fibril size measurement, and electron microscopy results revealed that conjugates suppress fibrillogenesis in HEWL. The highest amyloid inhibition activity obtained against C-PVP and C-PVP-Au was 31 µg.mL-1 and 30 µg.mL-1, respectively. The dissociation activity for amyloid aggregation was observed against Q-PVP and Q-PVP-Au at 29 µg.mL-1 and 27 µg.mL-1, respectively. The antibacterial studies show significant antibacterial efficacy against Escherichia coli (E.coli) in the presence of C-PVP and C-PVP-Au. The substantial antibacterial potential of C-PVP@PVA and C-PVP-Au@PVA membranes shows promising wound healing applications. Membranes containing curcumin polymeric nanoparticles, gold nanoparticles, and polyvinyl alcohol (PVA) promote the antibacterial activity and wound healing activity to the wound of Drosophila. C-PVP-Au@PVA membrane healed the wound faster than the C-PVP@PVA, and it can be used for better results in wound healing. Thus, C-PVP-Au NP and C-PVP NP have higher bioavailability and stability and can act as multifunctional therapeutic agents for amyloid-related diseases and wound management systems.

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“…The authors suggested that the antidiabetic effect could be attributed to the direct correlation between the phytoconstituents surrounding PtNPs and α-amylase and/or α- glucosidase inhibitory action, excessive ROS production or imbalanced antioxidant protection mechanisms [ 96 ]. Furthermore, a significant decrease in glucose levels after PtNPs injection was observed in streptozotocin-induced diabetic rats [ 165 ]. Type 2 Diabetes is characterized by an increase in ROS production level, induced by chronic extracellular hyperglycemia as a result of a violation of the cell redox state, causing the abnormal expression of insulin sensitivity genes [ 166 , 167 ].…”

Section: Application Of Green Ptnpsmentioning

confidence: 99%

Green Synthesis of Platinum Nanoparticles for Biomedical Applications

Михайлова¹

2022

JFB

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The diverse biological properties of platinum nanoparticles (PtNPs) make them ideal for use in the development of new tools in therapy, diagnostics, and other biomedical purposes. “Green” PtNPs synthesis is of great interest as it is eco-friendly, less energy-consuming and minimizes the amount of toxic by-products. This review is devoted to the biosynthesis properties of platinum nanoparticles based on living organisms (bacteria, fungi, algae, and plants) use. The participation of various biological compounds in PtNPs synthesis is highlighted. The biological activities of “green” platinum nanoparticles (antimicrobial, anticancer, antioxidant, etc.), the proposed mechanisms of influence on target cells and the potential for their further biomedical application are discussed.

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Platinum as a Novel Nanoparticle for Wound Healing Model in Drosophila melanogaster

Cited by 9 publications

References 62 publications

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Polymeric curcumin nanospheres for lysozyme aggregation inhibition, antibacterial and wound healing applications

Green Synthesis of Platinum Nanoparticles for Biomedical Applications

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Platinum as a Novel Nanoparticle for Wound Healing Model in Drosophila melanogaster

Cited by 9 publications

References 62 publications

In Vivo Toxicological Analysis of MnFe2O4@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of MnFe2O4@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

Polymeric curcumin nanospheres for lysozyme aggregation inhibition, antibacterial and wound healing applications

Green Synthesis of Platinum Nanoparticles for Biomedical Applications

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In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use

In Vivo Toxicological Analysis of MnFe₂O₄@poly(tBGE-alt-PA) Composite as a Hybrid Nanomaterial for Possible Biomedical Use