pLDH level of clinically isolated Plasmodium vivax and detection limit of pLDH based malaria rapid diagnostic test

Jang, Jin Woo; Cho, Chi Hyun; Han, Eun Taek; An, Seong Soo A.; Lim, Chae Seung

doi:10.1186/1475-2875-12-181

Cited by 49 publications

(46 citation statements)

References 13 publications

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“…The three products with Pan-pLDH test lines showed contrasting results when tested with wild type P. vivax samples, with an overall detection limit of 10 ng/mL (RDT 13), 50 ng/mL (RDT 12), and 1000 ng/mL (RDT 10), suggesting that the sensitivity of Pan-pLDH test lines is very much product-dependent. The limit of detection of Pv-pLDH test lines of P. vivax isolates was more consistent with two products at 25 ng/mL and the third one at 50 ng/mL and in line with values reported in a previous study [16]. …”

Section: Discussionsupporting

confidence: 91%

Analytical sensitivity of current best-in-class malaria rapid diagnostic tests

Jiménez

Rees-Channer

Perera

et al. 2017

Malar J

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BackgroundRapid diagnostic tests (RDTs) are today the most widely used method for malaria diagnosis and are recommended, alongside microscopy, for the confirmation of suspected cases before the administration of anti-malarial treatment. The diagnostic performance of RDTs, as compared to microscopy or PCR is well described but the actual analytical sensitivity of current best-in-class tests is poorly documented. This value is however a key performance indicator and a benchmark value needed to developed new RDTs of improved sensitivity.MethodsThirteen RDTs detecting either the Plasmodium falciparum histidine rich protein 2 (HRP2) or the plasmodial lactate dehydrogenase (pLDH) antigens were selected from the best performing RDTs according to the WHO–FIND product testing programme. The analytical sensitivity of these products was evaluated using a range of reference materials including P. falciparum and Plasmodium vivax whole parasite samples as well as recombinant proteins.ResultsThe best performing HRP2-based RDTs could detect all P. falciparum cultured samples at concentrations as low as 0.8 ng/mL of HRP2. The limit of detection of the best performing pLDH-based RDT specifically detecting P. vivax was 25 ng/mL of pLDH.ConclusionThe analytical sensitivity of P. vivax and Pan pLDH-based RDTs appears to vary considerably from product to product, and improvement of the limit-of-detection for P. vivax detecting RDTs is needed to match the performance of HRP2 and Pf pLDH-based RDTs for P. falciparum. Different assays using different reference materials produce different values for antigen concentration in a given specimen, highlighting the need to establish universal reference assays.Electronic supplementary materialThe online version of this article (doi:10.1186/s12936-017-1780-5) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 91%

Analytical sensitivity of current best-in-class malaria rapid diagnostic tests

Jiménez

Rees-Channer

Perera

et al. 2017

Malar J

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“…Moreover, in this study most of our cases were P.vivax infected patients 64% followed by P.falciparum 36% as shown in table 2 as most of our cases were from southeast of Asia and the dominant species were P.vivax, which is similar to the results shown by Ngasala et al, [15]. The level of parasitaemia was higher in P.vivax cases as shown in figure 2, this result is matching with the finding of Jang et al, [16] as most of their cases were with high P.vivax Parasitaemia.…”

Section: Discussionsupporting

confidence: 82%

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2016

AJBVP

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Malaria presents a diagnostic challenge to laboratories especially in non-endemic malaria countries. Population were done locally and the accuracy was evaluated. In this study detected 61 (78%) malaria positive slides, while 65 (83%) and 64 (82%) positive samples were detected with Optimal-IT and OneStep Malaria Antigen Rapid Tests respectively. Optimal-IT sensitivity (98%), specificity (71%), positive predictive value (92%), negative predictive value (92%) and diagnostic accuracy (92%) whereas, one step RDT test showed 97%, 71 %, 92%, 86%, 91% respectively. However, the differences in detection rates of these diagnostic tests were not statistically significant (P>0.05). RDTs are considered the best option in health areas specially with limited laboratory services despite the problems related to their accuracy. However, this study showed that the accuracy of RDTs is not always satisfactory when performed in clinical laboratories.

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“…Another study that was performed at Combined Military Hospital Quetta by Ahmad et al (2003), concluded 100% OptiMAL sensitivity for both malaria species. Another study was performed in Belgium( non-endemic country for malaria ), reported sensitivity of 92.9% for the SD Bioline Malaria Ag Pf/Pan (Maltha et al, 2011) while in Korea, the researchers reported a sensitivity of RDT for P. vivax that ranged from 86.8% to 92.7% (Kim et al, 2011 ;Jang et al, 2013). The results of the present study are more or less similar in sensitivity to most of the above mentioned studies performed worldwide for malaria diagnosis.…”

Section: Discussionsupporting

confidence: 72%

“…The optiMAL dipstick test is a rapid immunochromatographic assay which detects P. falciparum-specific parasite Lactate Dehydragenase enzyme (pLDH) and a parasite Lactate Dehydrogenase (pLDH) common to the four Plasmodium species (Piper et al, 1999). Another RDT which is used in malaria surveys is SD Bioline Malaria Ag Pf/Pan test (Kim et al, 2011;Jang et al, 2013;Kosack et al, 2013;Mendoza et al, 2013). Recently synthesized RDTs are manufactured using different target antigens to be suitable for specific detection of malaria in different areas (Parra et al, 1991;Garcia, 1996;.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Two Rapid Diagnostic Tests with Conventional Microscopy for Detection of Malaria parasites

2017

Aust. J. Basic & Appl. Sci.

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Malaria is a major cause of death mostly in tropical and sub-tropical countries. The constant increase of population movements, leads to spread of infections previously limited to endemic areas. In this urgent situation, efficient diagnostic method is essential for the management and control of malaria. The aim of the present study was to compare two RDTs with conventional microscopy as diagnostic tools for detection of falciparum and non falciparum malaria in travelers and migrating people from malaria endemic areas to Egypt. A total of 112 blood samples were studied by light microscopy, microscopy positive malaria samples were tested by two malaria RDTs. Malarial parasites were observed in 28 cases(25%) with conventional light microscopy, of which the OptiMAL test detected 27 positive cases (96%) and only 26 cases ( 93 %) were positive by Bio Tina Pf/Pan Mal test. Microscopy showed 15 P. falciparum and 13 P. vivax cases. The OptiMAL dipstick revealed 15 positive P. falciparum and 12 positive blood samples for non falciparum malaria while the Bio Tina Pf/Pan Mal test revealed 14 P. falciparum and 12 non P. falciparum malaria. The OptiMAL sensitivities in the present study was 100% and 92% for P. falciparum and non falciparum malaria respectively while the Bio Tina Pf/Pan Mal method sensitivities was 93% and 92 % for P. falciparum and non falciparum malaria respectively. Microscopic examination for malaria parasite by thin and thick blood films is the gold standard technique for malaria diagnosis.

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pLDH level of clinically isolated Plasmodium vivax and detection limit of pLDH based malaria rapid diagnostic test

Cited by 49 publications

References 13 publications

Analytical sensitivity of current best-in-class malaria rapid diagnostic tests

Analytical sensitivity of current best-in-class malaria rapid diagnostic tests

Untitled

Comparison of Two Rapid Diagnostic Tests with Conventional Microscopy for Detection of Malaria parasites

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