Ploidy instability in long-term in vitro cultures of Coffea arabica L. monitored by flow cytometry

Clarindo, Wellington Ronildo; Carvalho, Carlos Roberto; Mendonça, Maria Andréia Corrěa

doi:10.1007/s10725-012-9740-0

Cited by 21 publications

(10 citation statements)

References 27 publications

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“…Similarly, Clarindo et al (2012) suggested a limit of less than 4 months storage of coffee cell aggregate suspensions for true-to-type mass propagation as ploidy instability was noticed in long-term in vitro culture. Similarly when Farahani et al (2011) raised olive cultivars, under in vitro conditions, through internode cuttings, significant difference was observed in morphological characters among the regenerated plants after seventh subculture, which was later confirmed by RAPD analysis.…”

Section: Sources Of Variations Detected In Plant Tissue Culturementioning

confidence: 94%

“…A wide variety of tools are available for the detection and characterization of somaclonal variants which are primarily based on the differences in morphological traits (Pérez et al 2009, 2011; Nhut et al 2013), cytogenetical analysis for the determination of numerical and structural variation in the chromosomes (Clarindo et al 2012; Currais et al 2013; Abreu et al 2014), biochemical (Vujovic et al 2010; Kar et al 2014), molecular DNA markers (Krishna and Singh 2007; Pathak and Dhawan 2012; Hossain et al 2013; Bello-Bello et al 2014) or their combinations (Horáček et al 2013; Dey et al 2015; Stanišić et al 2015). The best test for assessing somaclonal variation is to fruit out the plants and conduct an extensive horticultural evaluation, which is unfortunately a long-term endeavor with woody fruit crops, particularly (Grosser et al 1996).…”

Section: Identification Of Variation In Tissue Culturementioning

confidence: 99%

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Somaclonal variations and their applications in horticultural crops improvement

Krishna¹,

Alizadeh

Singh³

et al. 2016

3 Biotech

341

216

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The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerated plants. However, plant tissue culture may generate genetic variability, i.e., somaclonal variations as a result of gene mutation or changes in epigenetic marks. The occurrence of subtle somaclonal variation is a drawback for both in vitro cloning as well as germplasm preservation. Therefore, it is of immense significance to assure the genetic uniformity of in vitro raised plants at an early stage. Several strategies have been followed to ascertain the genetic fidelity of the in vitro raised progenies comprising morpho-physiological, biochemical, cytological and DNA-based molecular markers approaches. Somaclonal variation can pose a serious problem in any micropropagation program, where it is highly desirable to produce true-to-type plant material. On the other hand, somaclonal variation has provided a new and alternative tool to the breeders for obtaining genetic variability relatively rapidly and without sophisticated technology in horticultural crops, which are either difficult to breed or have narrow genetic base. In the present paper, sources of variations induced during tissue culture cycle and strategies to ascertain and confirm genetic fidelity in a variety of in vitro raised plantlets and potential application of variants in horticultural crop improvement are reviewed.

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Section: Sources Of Variations Detected In Plant Tissue Culturementioning

confidence: 94%

Section: Identification Of Variation In Tissue Culturementioning

confidence: 99%

Somaclonal variations and their applications in horticultural crops improvement

Krishna¹,

Alizadeh

Singh³

et al. 2016

3 Biotech

341

216

View full text Add to dashboard Cite

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“…For the same purpose, different approaches have demonstrated that FCM is practical, quick and reliable (Roy et al 2001, Clarindo et al 2012. Further, several samples can be analyzed on a single day (Doležel et al 2007).…”

Section: Nuclear 2c Value and Karyotype Of 'Santa Cruz Kada Gigante'mentioning

confidence: 99%

<i>In Vitro</i> Polyploidization in<i> Solanum lycopersicum</i> Mill. ‘Santa Cruz Kada Gigante'

et al. 2014

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Summary Solanum lycopersicum Mill. is a model plant for biological approaches and includes accessions with great agronomic relevance, such as the Santa Cruz Kada Gigante . For development of new S. lycopersicum accessions, in vitro tissue culture has proved relevant, contributing to the establishment of germplasm banks and breeding programs. In spite of the vast amount of tissue culture research already carried out in S. lycopersicum, in vitro polyploidization is still emerging. Thus, this study aimed to induce polyploid plantlets from Santa Cruz Kada Gigante from shoot tip explants. This accession presented 2n=2x=24 chromosomes and 2C=2.00 picograms of nuclear DNA. Hence, no preexisting variation was identified, and Santa Cruz Kada Gigante was considered appropriate for in vitro polyploidization. This procedure provided tetraploid (4x) and mixoploid (2x/4x and 2x/4x/8x) plantlets, which were identified by flow cytometry (FCM) and multiplied in vitro. Based on these results, the tissue culture procedure provided polyploid Santa Cruz Kada Gigante plantlets, which represent a new source of variability for breeding programs of S. lycopersicum.

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“…Cytogenetic (Mujib et al 2007;Clarindo et al 2012) and FCM (Pinto et al 2010;Clarindo et al 2012) techniques have been successfully applied to assess the karyotype and ploidy stability of in vitro regenerated plantlets in several species. FCM has been considered a rapid and efficient tool for detection of somaclonal variants, allowing to measure the ploidy level of a large number of samples in a short period of time (Bairu et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Embryogenic potential of immature zygotic embryos of Passiflora: a new advance for in vitro propagation without plant growth regulators

Ferreira

Sattler

Carvalho

et al. 2015

Plant Cell Tiss Organ Cult

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In vitro strategies for Passiflora have been developed owing to its economic and ecological importance. However, plantlet regeneration through somatic embryogenesis has presented some problems, such as the reproducibility of the protocol and formation of abnormal embryos and plantlets. Thus, this study aimed to establish a protocol exploring the embryogenic potential of immature zygotic embryos (IZE) of the wild species Passiflora miniata Vanderpl. and Passiflora speciosa Gardn. Friable calli, which formed on the abaxial surface of the cotyledons, yielded globular, heart-shaped, torpedo and cotyledonary somatic embryos, characterising the embryogenic response as asynchronous. A high percentage of normal regenerants (90 %) was obtained from IZE in media lacking 2,4-dichlorophenoxyacetic acid (2,4-D) in comparison to the value of normal plantlets (60 %) regenerated from mature zygotic embryos inoculated in media with 2,4-D. This result demonstrates that IZE of P. miniata and P. speciosa possess sufficient levels of endogenous phytohormones to trigger a high rate of indirect somatic embryogenesis. All regenerated plantlets had the same genome size and chromosome number as the explant donor plants. Therefore, the indirect embryogenic pathway, employing IZE inoculated into media free of growth regulators, did not cause changes in the karyotype and morphology. Based on these results, IZE should be considered as explant for the establishment of somatic embryogenesis in other species. Besides, a new, reliable and relatively rapid protocol to recover plantlets of P. miniata and P. speciosa yielded several plants, which were acclimatised and used for ornamental purposes and breeding programs, and for reintroduction into biological reserves.

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Ploidy instability in long-term in vitro cultures of Coffea arabica L. monitored by flow cytometry

Cited by 21 publications

References 27 publications

Somaclonal variations and their applications in horticultural crops improvement

Somaclonal variations and their applications in horticultural crops improvement

<i>In Vitro</i> Polyploidization in<i> Solanum lycopersicum</i> Mill. ‘Santa Cruz Kada Gigante'

Embryogenic potential of immature zygotic embryos of Passiflora: a new advance for in vitro propagation without plant growth regulators

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