Pluripotent Stem Cell Differentiation Toward Functional Basal Stratified Epithelial Cells

Soares, Eduardo V.; Zhou, Huiqing

doi:10.1007/7651_2020_301

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…Referring to the induction procedure in the literature, 40 cells were gently resuspended with 2 mL of Essential 8 medium containing RevitaCell™ supplement. Cells were plated onto pre‐Geltrex™ LDEV‐Free growth factor‐incubated plates and maintained in Essential 8 medium.…”

Section: Methodsmentioning

confidence: 99%

“…GFP fluorescence intensity was observed under a fluorescence microscope, and transfection efficiency was calculated by randomly taking eight 200× microscopic fields. RT-qPCR and Western blot were performed to detect VEGF 165 expression in iPSCs and evaluate the overexpression efficiency.Referring to the induction procedure in the literature,40 cells were gently resuspended with 2 mL of Essential 8 medium containing RevitaCell™ supplement. Cells were plated onto pre-Geltrex™ LDEV-Free growth factor-incubated plates and maintained in Essential 8 medium.…”

mentioning

confidence: 99%

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Feasibility of repairing skin defects by VEGF₁₆₅ gene‐modified iPS‐HFSCs seeded on a 3D printed scaffold containing astragalus polysaccharide

Huang

et al. 2023

J Cellular Molecular Medi

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The preparation of biodegradable scaffolds loaded with cells and cytokine is a feature of tissue‐engineered skin. IPSCs‐based tissue‐engineered skin treatment for wound repair is worth exploring. Healthy human skin fibroblasts were collected and reprogrammed into iPSCs. After gene modification and induction, CK19+/Integrinβ1+/CD200+ VEGF165 gene‐modified iPS‐HFSCsGFP were obtained and identified by a combination of immunofluorescence and RT‐qPCR. Astragalus polysaccharide‐containing 3D printed degradable scaffolds were prepared and co‐cultured with VEGF165 gene‐modified iPS‐HFSCsGFP, and the biocompatibility and spatial structure of the tissue‐engineered skin was analysed by cell counting kit‐8 (CCK8) assay and scanning electron microscopy. Finally, the tissue‐engineered skin was transplanted onto the dorsal trauma of nude mice, and the effect of tissue‐engineered skin on the regenerative repair of total skin defects was evaluated by a combination of histology, immunohistochemistry, immunofluorescence, RT‐qPCR, and in vivo three‐dimensional reconstruction under two‐photon microscopy. CK19+/Integrinβ1+/CD200+ VEGF165 gene‐modified iPS‐HFSCsGFP, close to the morphology and phenotype of human‐derived hair follicle stem cells, were obtained. The surface of the prepared 3D printed degradable scaffold containing 200 μg/mL astragalus polysaccharide was enriched with honeycomb‐like meshwork, which was more conducive to the proliferation of the resulting cells. After tissue‐engineered skin transplantation, combined assays showed that it promoted early vascularization, collagen and hair follicle regeneration and accelerated wound repair. VEGF165 gene‐modified iPS‐HFSCsGFP compounded with 3D printed degradable scaffolds containing 200 μg/mL astragalus polysaccharide can directly and indirectly participate in vascular, collagen, and hair follicle regeneration in the skin, achieving more complete structural and functional skin regenerative repair.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Feasibility of repairing skin defects by VEGF₁₆₅ gene‐modified iPS‐HFSCs seeded on a 3D printed scaffold containing astragalus polysaccharide

Huang

et al. 2023

J Cellular Molecular Medi

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“…Induced keratinocytes (iKC) derived from gene-corrected iPSCs were grafted onto immunodeficient mice, and two months post grafting a normal expression of COL7A1 is shown (Jackow et al, 2019). Although generation of genome edited iPSCs is relatively easy, differentiation from iPSC towards iKC, especially for resembling primary keratinocytes, is less straightforward (Kogut et al, 2014, Sah et al, 2021, Soares and Zhou, 2020. In addition, iPSC-derived keratinocytes are often immature, as compared to primary keratinocytes derived from the skin, which is a common feature of many iPSC-derived cells (Friedman et al, 2018, Soares et al, 2019.…”

Section: Keratinocytes Derived From Crispr-cas9 Edited Ipscsmentioning

confidence: 99%

CRISPR-Cas9‒Based Genomic Engineering in Keratinocytes: From Technology to Application

et al. 2022

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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A Systematic Review of Stem Cell Differentiation into Keratinocytes for Regenerative Applications

Hazrati,

Davaran,

Keyhanvar

et al. 2023

Stem Cell Rev and Rep

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Pluripotent Stem Cell Differentiation Toward Functional Basal Stratified Epithelial Cells

Cited by 3 publications

References 16 publications

Feasibility of repairing skin defects by VEGF₁₆₅ gene‐modified iPS‐HFSCs seeded on a 3D printed scaffold containing astragalus polysaccharide

Feasibility of repairing skin defects by VEGF₁₆₅ gene‐modified iPS‐HFSCs seeded on a 3D printed scaffold containing astragalus polysaccharide

CRISPR-Cas9‒Based Genomic Engineering in Keratinocytes: From Technology to Application

A Systematic Review of Stem Cell Differentiation into Keratinocytes for Regenerative Applications

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Pluripotent Stem Cell Differentiation Toward Functional Basal Stratified Epithelial Cells

Cited by 3 publications

References 16 publications

Feasibility of repairing skin defects by VEGF165 gene‐modified iPS‐HFSCs seeded on a 3D printed scaffold containing astragalus polysaccharide

Feasibility of repairing skin defects by VEGF165 gene‐modified iPS‐HFSCs seeded on a 3D printed scaffold containing astragalus polysaccharide

CRISPR-Cas9‒Based Genomic Engineering in Keratinocytes: From Technology to Application

A Systematic Review of Stem Cell Differentiation into Keratinocytes for Regenerative Applications

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Product

Resources

About

Feasibility of repairing skin defects by VEGF₁₆₅ gene‐modified iPS‐HFSCs seeded on a 3D printed scaffold containing astragalus polysaccharide

Feasibility of repairing skin defects by VEGF₁₆₅ gene‐modified iPS‐HFSCs seeded on a 3D printed scaffold containing astragalus polysaccharide