PM Q-probe: A fluorescent binding protein that converts many antibodies to a fluorescent biosensor

Dong, Jinhua; Miyake, Chihiro; Yasuda, Takanobu; Oyama, Hiroyuki; Morita, Izumi; Tsukahara, Tomoya; Takahashi, Masaki; Jeong, Hee‐Jin; Kitaguchi, Tetsuya; Kobayashi, N.; Ueda, Hiroshi

doi:10.1016/j.bios.2020.112425

Cited by 21 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, we developed a probe (PM Q-probe) that can easily convert many antibodies, including commercially available ones, into fluorescent biosensors using a recently discovered antibody-binding protein Protein M (PM) [52]. The PM, isolated from human mycoplasma, was found to bind strongly (K d~l ow nM) to a wide range of antibodies at the light chain, and is considered to disrupt the immune system by structurally blocking the binding of antibodies to (protein) antigens [53].…”

Section: Q-probementioning

confidence: 99%

See 1 more Smart Citation

Recent Advances in Quenchbody, a Fluorescent Immunosensor

Dong

Ueda

2021

Sensors

Self Cite

View full text Add to dashboard Cite

The detection of viruses, disease biomarkers, physiologically active substances, drugs, and chemicals is of great significance in many areas of our lives. Immunodetection technology is based on the specificity and affinity of antigen–antibody reactions. Compared with other analytical methods such as liquid chromatography coupled with mass spectrometry, which requires a large and expensive instrument, immunodetection has the advantages of simplicity and good selectivity and is thus widely used in disease diagnosis and food/environmental monitoring. Quenchbody (Q-body), a new type of fluorescent immunosensor, is an antibody fragment labeled with fluorescent dyes. When the Q-body binds to its antigen, the fluorescence intensity increases. The detection of antigens by changes in fluorescence intensity is simple, easy to operate, and highly sensitive. This review comprehensively discusses the principle, construction, application, and current progress related to Q-bodies.

show abstract

Section: Q-probementioning

confidence: 99%

“…The normal concentration range in blood for each dose-response is shown in parentheses. Modified with permission from[52].…”

mentioning

confidence: 99%

Recent Advances in Quenchbody, a Fluorescent Immunosensor

Dong

Ueda

2021

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…The operation of the Q-body assay is simple, because only the fluorescence-quenched body immunosensor is mixed with the sample to be tested, and the determination time is tens of seconds to a few minutes 21 , 22 . To date, Q-body technology has been developed for the detection of small molecules 23 , 24 , peptides 25 , and proteins or their phosphorylation 26 – 29 , and a variety of Q-body preparation methods have been developed 30 – 32 .…”

Section: Introductionmentioning

confidence: 99%

Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensors

Zhu

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Since late 2019, the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the resultant spread of COVID-19 have given rise to a worldwide health crisis that is posing great challenges to public health and clinical treatment, in addition to serving as a formidable threat to the global economy. To obtain an effective tool to prevent and diagnose viral infections, we attempted to obtain human antibody fragments that can effectively neutralize viral infection and be utilized for rapid virus detection. To this end, several human monoclonal antibodies were isolated by bio-panning a phage-displayed human antibody library, Tomlinson I. The selected clones were demonstrated to bind to the S1 domain of the spike glycoprotein of SARS-CoV-2. Moreover, clone A7 in Fab and IgG formats were found to effectively neutralize the binding of S protein to angiotensin-converting enzyme 2 in the low nM range. In addition, this clone was successfully converted to quench-based fluorescent immunosensors (Quenchbodies) that allowed antigen detection within a few minutes, with the help of a handy fluorometer.

show abstract

“…Specific binding of the antigen to the antibody results in quenching of the fluorescent dye inside the antibody. The dye is displaced to the outside of the antibody, where de-quenching occurs, resulting in an antigen-dependent increase in fluorescence intensity ( Dong et al, 2020a ).…”

Section: Introductionmentioning

confidence: 99%

Isolation of a Monoclonal Antibody and its Derived Immunosensor for Rapid and Sensitive Detection of 17β-Estradiol

Dong

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Estrogens are effective for stimulating several functions in living organisms and for regulating cancer development by promoting cell proliferation. Estradiol can disrupt the reproductive and endocrine systems, leading to the development of various diseases. In this study, the monoclonal antibody ESC9 was developed by immunizing mice with a 17β-estradiol (E2) conjugate, preparing an antibody phage display library, and screening monoclonal antibodies from the prepared library. An antibody with the same sequence as that of ESC9 has not been reported previously. The equilibrium dissociation constant between ESC9 and E2 was found to be 43.3 nM. Additionally, we generated an ESC9-derived immunosensor named as the ESC9 Quenchbody (Q-body), which can rapidly and sensitively detect E2. The assay can be completed within 2 min with a limit of detection of 3.9 pg/ml and half-maximal effective concentration of 154.0 ng/ml. Serum E2 levels were measured using the ESC9 Q-body without pretreatment with serum and with a high recovery rate of 83.3–126.7%. The Q-body immunosensor shows potential for clinical applications based on its excellent detection speed and sensitivity.

show abstract

PM Q-probe: A fluorescent binding protein that converts many antibodies to a fluorescent biosensor

Cited by 21 publications

References 28 publications

Recent Advances in Quenchbody, a Fluorescent Immunosensor

Recent Advances in Quenchbody, a Fluorescent Immunosensor

Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensors

Isolation of a Monoclonal Antibody and its Derived Immunosensor for Rapid and Sensitive Detection of 17β-Estradiol

Contact Info

Product

Resources

About