PMSG and HCG hormones effect on the development and growth of ovarian follicles

Zolbin, Masoumeh Majidi; Nematin, Sepidah; Daghigh, Faeze; Namdar, Fariba; Madadian, Mahmoodreza; Abbasi, Mohsen; Mogahi, Seyed Mohammad Hosein Noori; Takzaree, Nasrin

doi:10.22317/jcms.03201811

Cited by 5 publications

(1 citation statement)

References 16 publications

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“…While both PMSG and LHRHa can induce estrus synchronization, our study showed they have different effects on fetal development. PMSG has primarily FSH-like and LH-like activity in non-equids 43 , 44 .…”

Section: Discussionmentioning

confidence: 99%

Scheduled simple production method of pseudopregnant female mice for embryo transfer using the luteinizing hormone-releasing hormone agonist

Sari

Hilario

Yuri

et al. 2022

Sci Rep

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The use of mice as experimental animal models has been a practice since the development of genetically engineered mouse models (GEMMs) in the early 1980s. New technologies, including genome editing, have helped in the time- and cost-efficient generation of GEMMs. However, methods for preparing pseudopregnant females, essential for the generation of GEMMs, remain less advanced. This study proposes a new method to achieve simple production of pseudopregnant female mice using a luteinizing hormone-releasing hormone agonist (LHRHa). A 20 µg LHRHa/mouse was identified as the best dose for inducing estrus synchronization. However, the frequency of copulation was 40% on a single injection. With sequential injections of 20 µg LHRHa/mouse on Days-1 and -2, followed by pairing on Day-5, 74% of LHRHa-treated females copulated with male mice. Moreover, LHRHa treatment did not affect fetal and postnatal development. Eventually, successful generation of offspring via embryo transfer was attained using LHRHa-treated pseudopregnant females. LHRHa administration method is efficient in producing pseudopregnant female mice for the generation of GEMMs, and we expect that it will contribute towards advancing the clinical research.

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Section: Discussionmentioning

confidence: 99%

Scheduled simple production method of pseudopregnant female mice for embryo transfer using the luteinizing hormone-releasing hormone agonist

Sari

Hilario

Yuri

et al. 2022

Sci Rep

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Stereological and functional study on ovarian follicles in surri mice following soybean consumption

et al. 2022

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Angka kebuntingan sapi perah dengan korpus luteum persisten setelah pemberian kombinasi prostaglandin F2α dan gonadotropin

Azwani

Srianto²,

Madyawati³

et al. 2021

Ovz

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This case study aims to determine the effect of a combination of prostaglandin F2α (PGF2α) with Gonadotropin (PG-600), and human chorionic gonadotropin (hCG) on the estrus and pregnancy rates in Holstein Friesian (HF) cows with persistent corpus luteum (PCL). A total of 15 Holstein Friesian (HF) dairy cows aged 3-5 years with body condition score (BCS) 5-7 (scale 1-9) diagnosed with PCL, were injected with 7.5 mg PGF2α intra-vulvar submucosa. Cows were then divided into three treatment groups. Group P0 was not given the combination treatment, while P1 group was injected with a combination of 300 IU PG-600 and 150 IU hCG, meanwhile P2 group was was injected with a combination of 300 IU PG-600 and 300 IU hCG intra-muscularly. The PGF2α and PG-600 injections were carried out on the same day PCL was diagnosed. Meanwhile, the injection of hCG was carried out at the same time as the AI. The Pregnancy examination was performed 90 days after AI through rectal palpation. The results showed that all treated cows were estrus observed with the fastest onset of estrus was the P2 group, that was significantly different (p <0.05) compared to the P0 and P1 groups. All treated cows were diagnosed as pregnant. It can be concluded that the administration of PGF2α or its combination with gonadotropins in HF cows with PCL could result in 100% estrus and pregnancy rates.

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PMSG and HCG hormones effect on the development and growth of ovarian follicles

Cited by 5 publications

References 16 publications

Scheduled simple production method of pseudopregnant female mice for embryo transfer using the luteinizing hormone-releasing hormone agonist

Scheduled simple production method of pseudopregnant female mice for embryo transfer using the luteinizing hormone-releasing hormone agonist

Stereological and functional study on ovarian follicles in surri mice following soybean consumption

Angka kebuntingan sapi perah dengan korpus luteum persisten setelah pemberian kombinasi prostaglandin F2α dan gonadotropin

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