Pneumocystosis

Salfelder, K.; Schwarz, Jan

doi:10.1001/archpedi.1967.02090270149023

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Epidemiology1

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1971

1992

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Cited by 13 publications

(1 citation statement)

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“…In Leipzig, 1,000 cases were seen between 1945 and 1953. 9 During the period from 1953 to 1957, 2,000 cases were registered in Czechoslovakia, with 412 cases confirmed at autopsy." The European cases were primarily in debilitated or premature infants in institutions.…”

Section: Epidemiologymentioning

confidence: 99%

Current Status of Laboratory Diagnosis ofPneumocystis Carinii Pneumonitis

Hughes

1975

CRC Critical Reviews in Clinical Laboratory Sciences

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Section: Epidemiologymentioning

confidence: 99%

Current Status of Laboratory Diagnosis ofPneumocystis Carinii Pneumonitis

Hughes

1975

CRC Critical Reviews in Clinical Laboratory Sciences

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Immunology of Pneumocystis carinii

Hughes

1982

Immunology of Human Infection

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Ultrastructural detection of carbohydrates in the pellicle of Pneumocystis carinii

1988

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The presence of carbohydrates in the pellicle of Pneumocystis carinii was demonstrated by methenamine silver (MS) and thiocarbohydrazide-silver proteinate (TCH-SP) staining techniques. An intense, positive reaction with MS was observed on both the electron-dense outer layer and the electron-lucent middle layer of the pellicle, whereas in the case of TCH-SP only the outer layer stained well. The middle layer did not stain as intensely with TCH-SP as with MS. This observation indicates that the pellicle of P. carinii contains carbohydrates but that the outer and middle layers differ in carbohydrate composition. The binding sites of concanavalin A (Con A) and Macura pomifera (MPA) lectins were elucidated on the pellicle of P. carinii using colloidal gold as a marker. In a preembedding staining method using Con A and MPA lectins, the adherence of gold particles was observed on the surface of all stages of the parasite. The homogeneous distribution of the gold particles indicates that the outer electron-dense layer contains both Con A- and MPA-specific carbohydrates. In postembedding staining with Con A, the adherence of gold particles was found on both the outer and middle layers, whereas in the case of MPA only the outer layer was labeled with gold particles. These results indicate that the electron-dense outer layer contains a considerable amount of carbohydrates specific for these lectins, whereas the electron-lucent middle layer seems to lack MPA-specific carbohydrates.

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Pneumocystosis

Cited by 13 publications

References 23 publications

Current Status of Laboratory Diagnosis ofPneumocystis Carinii Pneumonitis

Current Status of Laboratory Diagnosis ofPneumocystis Carinii Pneumonitis

Immunology of Pneumocystis carinii

Ultrastructural detection of carbohydrates in the pellicle of Pneumocystis carinii

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