Podosomes and invadopodia: tools to breach vascular basement membrane

Seano, Giorgio; Primo, Luca

doi:10.1080/15384101.2015.1026523

Cited by 45 publications

(40 citation statements)

References 27 publications

(33 reference statements)

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“…Podosome rosettes that are characterized by a typical circular arrangement have been implicated in angiogenesis and vascular branching (9). Because podosome rosettes were found to be most frequent in endothelial cells (13), we concentrated our studies on rosettes and their corresponding imprints in the matrix. Podosome rosettes were found to be expressed constitutively in HUVECs, which confirmed earlier observations (23, 24).…”

Section: Discussionmentioning

confidence: 99%

Impaired endothelial shear stress induces podosome assemblyviaVEGF up‐regulation

et al. 2016

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Podosomes are dynamic cytoskeletal membrane structures with local adhesive and proteolytic activity. They are critically involved in angiogenesis and vascular adaptive growth. Here, we studied in HUVECs and murine small vessels whether shear stress controls podosome assembly and local proteolytic activity. Podosomes were characterized by immunohistochemistry, and their proteolytic activity was assessed as degradation imprints in fluorescent gelatin that was used as growth substrate. Compared with controls (10 dyn/cm 2 ), the number of podosomes formed per time was doubled when cells were exposed to low shear stress (0.3 dyn/cm 2

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Section: Discussionmentioning

confidence: 99%

Impaired endothelial shear stress induces podosome assemblyviaVEGF up‐regulation

et al. 2016

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“…The formation of podosomes, which share similarity with invadopodia and are specialized adhesive and invasive structures involved in 3D migration of certain cell types (e.g. macrophages, endothelial cells), also depends on microtubules (Linder et al, 2000;Maridonneau-Parini, 2014;Seano and Primo, 2015). Together, these studies suggest a universal role for microtubules in mesenchymal cell protrusion and elongation within soft environments.…”

Section: Microtubule Requirement In Cell Morphogenesis and Motility Imentioning

confidence: 99%

Microtubules in 3D cell motility

Bouchet

Akhmanova

2017

Journal of Cell Science

104

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Three-dimensional (3D) cell motility underlies essential processes, such as embryonic development, tissue repair and immune surveillance, and is involved in cancer progression. Although the cytoskeleton is a well-studied regulator of cell migration, most of what we know about its functions originates from studies conducted in twodimensional (2D) cultures. This research established that the microtubule network mediates polarized trafficking and signaling that are crucial for cell shape and movement in 2D. In parallel, developments in light microscopy and 3D cell culture systems progressively allowed to investigate cytoskeletal functions in more physiologically relevant settings. Interestingly, several studies have demonstrated that microtubule involvement in cell morphogenesis and motility can differ in 2D and 3D environments. In this Commentary, we discuss these differences and their relevance for the understanding the role of microtubules in cell migration in vivo. We also provide an overview of microtubule functions that were shown to control cell shape and motility in 3D matrices and discuss how they can be investigated further by using physiologically relevant models.

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“…NF‐κB‐inducing kinase has similarly been found to activate Drp1 and thereby promote mitochondria redistribution to the leading edges of migrating cancer cells (Jung et al, ). Invadopodia are protrusive structures used by migrating cancer cells to penetrate tissues and establish new sites of tumor growth (Seano and Primo, ). Invadopodia share structural and molecular similarities with neuronal growth cones (Santiago‐Medina et al, ).…”

Section: Introductionmentioning

confidence: 99%

The role of mitochondria in axon development and regeneration

Smith

Gallo

2017

Developmental Neurobiology

146

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Mitochondria are dynamic organelles that undergo transport, fission, and fusion. The three main functions of mitochondria are to generate ATP, buffer cytosolic calcium, and generate reactive oxygen species. A large body of evidence indicates that mitochondria are either primary targets for neurological disease states and nervous system injury, or are major contributors to the ensuing pathologies. However, the roles of mitochondria in the development and regeneration of axons have just begun to be elucidated. Advances in the understanding of the functional roles of mitochondria in neurons had been largely impeded by insufficient knowledge regarding the molecular mechanisms that regulate mitochondrial transport, stalling, fission/fusion, and a paucity of approaches to image and analyze mitochondria in living axons at the level of the single mitochondrion. However, technical advances in the imaging and analysis of mitochondria in living neurons and significant insights into the mechanisms that regulate mitochondrial dynamics have allowed the field to advance. Mitochondria have now been attributed important roles in the mechanism of axon extension, regeneration, and axon branching. The availability of new experimental tools is expected to rapidly increase our understanding of the functions of axonal mitochondria during both development and later regenerative attempts. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 78: 221-237, 2018.

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Podosomes and invadopodia: tools to breach vascular basement membrane

Cited by 45 publications

References 27 publications

Impaired endothelial shear stress induces podosome assemblyviaVEGF up‐regulation

Impaired endothelial shear stress induces podosome assemblyviaVEGF up‐regulation

Microtubules in 3D cell motility

The role of mitochondria in axon development and regeneration

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