POF1B Localizes to Desmosomes and Regulates Cell Adhesion in Human Intestinal and Keratinocyte Cell Lines

Crespi, Alice; Bertoni, Alessandra; Ferrari, Ilaria; Padovano, Valeria; Mina, Pamela Della; Berti, Emilio; Villa, Antonello; Pietrini, Grazia

doi:10.1038/jid.2014.327

Cited by 9 publications

(5 citation statements)

References 30 publications

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“…The gene encoding POF1B was critical for ovarian function ( 37 ). However, its impact was also confirmed in the regulation of cell adhesion in human intestinal cell lines ( 38 ). CLMN has been shown to regulate the cell cycle in a report involving neuroblastoma cells ( 39 ).…”

Section: Discussionmentioning

confidence: 93%

Identification of candidate genes and long non-coding RNAs associated with the effect of ATP5J in colorectal cancer

et al. 2018

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The incidence and development of colorectal cancer (CRC) is a process with multiple gene interactions. We have previously demonstrated that ATP synthase-coupling factor 6, mitochondrial (ATP5J) is associated with CRC migration and 5-fluorouracil resistance; nevertheless, the exact molecular mechanism remains unclear. The following study uses microarray and bioinformatics methods to identify candidate genes and long non-coding RNAs (lncRNAs) in CRC cells (two pairs) with upregulated and downregulated ATP5J. Briefly, a total of 2,190 differentially expressed mRNAs (DEmRNAs) were sorted. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed for 4 DEmRNAs to validate the results of microarray analysis. Functional annotation and pathway enrichment were analyzed for DEmRNAs using the Database for Annotation, Visualization and Integrated Discovery. Significantly enriched pathways included the regulation of gene expression and cell growth. The protein-protein interaction network was constructed, and AKT serine/threonine kinase 2 (AKT2) was considered as one of the hub genes. For further analysis, 51 DEmRNAs and 30 DElncRNAs were selected that were positively or negatively associated with the expression of ATP5J in the two cell pairs. X-inactive specific transcript (XIST), premature ovarian failure 1B (POF1B) and calmin (CLMN) were identified in the DEmRNA-DElncRNA co-expression network. The expression of AKT2 and XIST in CRC cells was confirmed by RT-qPCR. To sum up, the candidate genes and lncRNAs, as well as potential signaling pathways, which were identified using integrated bioinformatics analysis, could improve the understanding of molecular events involved in the function of ATP5J in CRC.

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Section: Discussionmentioning

confidence: 93%

Identification of candidate genes and long non-coding RNAs associated with the effect of ATP5J in colorectal cancer

et al. 2018

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“…Several brush-border membranes such as meprin A and glutamate carboxypeptidase 2 were identified in all experiments. Other proteins related with barrier integrity such as protein POF1B, an important factor in the regulation of cell adhesion, were identified in all experiments. Besides regulators of cell adhesion molecules, haptoglobin, the zonulin precursor, was only identified in the absence of tannins.…”

Section: Resultsmentioning

confidence: 99%

New-Level Insights into the Effects of Grape Seed Polyphenols on the Intestinal Processing and Transport of a Celiac Disease Immunodominant Peptide

Pérez-Gregorio

Pereira

Dias

et al. 2021

J. Agric. Food Chem.

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The effect of three dietary tannins (procyanidin B3, B6, and T2) on the bioavailability of the 32-mer gliadin-derived immunogenic peptide was evaluated. An enterocyte-like Caco-2 cell line was used to mimic the epithelial transport of the 32-mer peptide, which was modeled by kinetic parameters with a mass spectrometry approach. The hydrolysis pattern on the enterocytes was analyzed, and the released peptides were quantified during the assay. The transport flux was dose-dependent. Along with procyanidin T2 and B6, procyanidin B3 promoted a significant inhibition mainly at the 100 μM peptide concentration. The hydrolysis efficiency was affected by procyanidins, while the cleavage pattern was suggested to be promoted by brush-border membranes at the apical compartment. The ability of procyanidins to molecularly bind to immunogenic peptides able to induce an adaptive response arose as a mechanism able to modulate their bioavailability, bioaccesibility, and further T CD4+ cell activation and expansion in a celiac disease (CD) model.

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“…showed that the binding of ALDOC to GSK-β leads to the β-Catenin complex collapse and destruction, thus increasing cytoplasmic and nuclear levels of β-catenin in patients with non-small-cell lung cancer ( 35 ). POF1B was located in the critical region for normal ovarian function and participated in encoding premature ovarian failure ( 36 ). Studies predicting the functions of proteins have shown that POF1B had a tight homology relationship with the myosin tail portion of the human myosin protein ( 37 ).…”

Section: Discussionmentioning

confidence: 99%

Development and validation of cancer-associated fibroblasts-related gene landscape in prognosis and immune microenvironment of bladder cancer

et al. 2023

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BackgroundsBladder cancer (BLCA) is one of the most prevalent cancers of the genitourinary system, the clinical outcomes of patients with BLCA are bad, and the morbidity rate is high. One of the key components of the tumor microenvironment (TME) is cancer-associated fibroblasts (CAFs) which are critically involved in BLCA tumorigenesis. Previous studies have shown the involvement of CAFs in tumor growth, cancer progression, immune evasion, angiogenesis, and chemoresistance in several cancers such as breast, colon, pancreatic, ovarian, and prostate cancers. However, only a few studies have shown the role of CAFs in the occurrence and development of BLCA.MethodsWe have retrieved and merged the data on RNA-sequencing of patients with BLCA from databases including “the Cancer Genome Atlas” and “Gene Expression Omnibus.” Next, we compared the differences in CAFs-related genes (CRGs) expression between normal and BLCA tissues. Based on CRGs expression, we randomly divided patients into two groups. Next, we determined the correlation between CAFs subtypes and differentially expressed CRGs (DECRGs) between the two subtypes. Furthermore, the “Gene Ontology” and “Kyoto Encyclopedia of Genes and Genomes pathway” enrichment analyses were conducted to determine the functional characteristics between the DECRGs and clinicopathology.ResultsWe identified five genes (POF1B, ARMCX1, ALDOC, C19orf33, and KRT13) using multivariate COX regression and “Least Absolute Shrinkage and Selection Operator (LASSO) COX regression analysis” for developing a prognostic model and calculating the CRGs-risk score. The TME, mutation, CSC index, and drug sensitivity were also analyzed.ConclusionWe constructed a novel five- CRGs prognostic model, which sheds light on the roles of CAFs in BLCA.

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POF1B Localizes to Desmosomes and Regulates Cell Adhesion in Human Intestinal and Keratinocyte Cell Lines

Cited by 9 publications

References 30 publications

Identification of candidate genes and long non-coding RNAs associated with the effect of ATP5J in colorectal cancer

Identification of candidate genes and long non-coding RNAs associated with the effect of ATP5J in colorectal cancer

New-Level Insights into the Effects of Grape Seed Polyphenols on the Intestinal Processing and Transport of a Celiac Disease Immunodominant Peptide

Development and validation of cancer-associated fibroblasts-related gene landscape in prognosis and immune microenvironment of bladder cancer

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