Polarization behavior of bone marrow-derived macrophages on charged P(VDF-TrFE) coatings

Wang, Zhiying; He, Xuzhao; Tang, Bolin; Chen, Xiaoyi; Dong, Lingqing; Cheng, Kui; Weng, Wenjian

doi:10.1039/d0bm01604g

Cited by 26 publications

(24 citation statements)

References 30 publications

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“…5 c). A Study showed that when the polarized membrane is covering the bone defect area, the polarized materials created a favorable osteo-immunomodulatory environment, thus promoting osteogenic differentiation [ 37 ]. Our study shown the same results that the JEM(−) membrane effectively promoted the osteogenic differentiation of rBMSCs in vitro and the bone defect was filled with homogeneous and consecutive regenerated mature bone in vivo .…”

Section: Resultsmentioning

confidence: 99%

Janus electro-microenvironment membrane with surface-selective osteogenesis/gingival healing ability for guided bone regeneration

Lai

Cheng

Ning

et al. 2022

Materials Today Bio

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Section: Resultsmentioning

confidence: 99%

Janus electro-microenvironment membrane with surface-selective osteogenesis/gingival healing ability for guided bone regeneration

Lai

Cheng

Ning

et al. 2022

Materials Today Bio

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“…The extraction and induction of BMDMs was performed as previously described (Z. Y. Wang et al., 2021). Briefly, cells were flushed out of the femurs of C57BL/6 mice, treated with ice cold red blood cell lysate buffer (Boster) for 3 min, centrifuged, the supernatant discarded, and then resuspended in α medium (Gibco) containing 10% fetal bovine serum (CellMax), 1% antibiotic solution (10,000 units per mL penicillin and 10,000 μg/mL streptomycin, Gibco), and 30 ng/ml M‐CSF (R&D Systems) and cultured at 37°C (5% CO 2 ).…”

Section: Methodsmentioning

confidence: 99%

Effects of electrical stimulation on cytokine‐induced macrophage polarization

Chen

et al. 2022

J Tissue Eng Regen Med

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Macrophages have two functionalized phenotypes, M1 and M2, and the regulation of M1/M2 polarization of macrophages is critical for tissue repair. Tissue-derived immune factors are considered the major drivers of macrophage polarization.Based on the main cytokine-induced polarization pathways, we tested the effect of electrical stimulation (ES) of macrophages on the regulation of M1/M2 polarization and a possible synergistic effect with the cytokines. Indium tin oxide (ITO) planar microelectrodes were used to produce ES under different voltages, frequencies and waveforms. We evaluated the influence of ES on the cytokine-induced M1/M2 polarization using mouse bone marrow-derived macrophages cultured with both lipopolysaccharide (LPS)/IFN-γ factors and IL-4 factors for M1 and M2, respectively. The results showed that ES promoted the cytokine-induced macrophage polarization. Importantly, we found that stimulation with a square waveform selectively promoted LPS/IFN-γ-induced M1 polarization, while stimulation with a sinusoidal waveform promoted both LPS/IFN-γ-induced M1, and IL-4-induced M2 polarization. Mechanistically, stimulation with a square waveform affected the intracellular ion concentration, whereas stimulation with a sinusoidal waveform promoted both the intracellular ion concentration and membrane receptors. We hereby establish an ES-mediated strategy for immunomodulation via macrophage polarization.

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“…[ 98 ] Wang et al. [ 99 ] designed several charged poly(vinylidene fluoride‐trifluoroethylene) coating materials with different potential strengths to study the polarization behavior of mouse BMDMs. The charged surface not only upregulated the polarization behavior of macrophages, but also guided the polarization phenotype.…”

Section: Biomaterials Modulate Macrophage Polarization To Promote Bon...mentioning

confidence: 99%

“…The positively charged GO-PEI complexes exhibited higher intracellular delivery efficiency, whereas the negatively charged counterparts showed an enhanced immune inflammatory response in macrophages. [98] Wang et al [99] designed several charged poly(vinylidene fluoride-trifluoroethylene) coating materials with different potential strengths to study the polarization behavior of mouse BMDMs. The charged surface not only upregulated the polarization behavior of macrophages, but also guided the polarization phenotype.…”

Section: Surface Chargementioning

confidence: 99%

Bone Repair Biomaterials: A Perspective from Immunomodulation

Wang

Zhang

Yan

et al. 2022

Adv Funct Materials

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With an increasingly aging society, bone defects and fractures have become significant threats to human health and quality of life. Currently, autologous and allogeneic bone grafts remain the "gold standard" for the clinical treatment of bone repair. However, bone transplantation is still clinically insufficient, owing to its substantial limitations, such as graft rejection, donor functional defects, and risk of infection. Therefore, a variety of biomaterials have been developed to facilitate bone repair. With the advancement of bone tissue engineering, the focus of research has transitioned from bioinert to bioactive biomaterials. However, many biomaterials have not achieved satisfactory therapeutic effects. In recent years, advancements in osteoimmunology have revealed that the immune system, of which macrophages are critical components, plays an essential regulatory role in bone regeneration. In this review, the role of macrophages in bone healing is explored and recent developments in biomaterials that promote bone regeneration by modulating macrophage polarization and improving the osteoimmune microenvironment are reviewed. This review provides a reference for the development and clinical application of bone repair biomaterials from the immunomodulation perspective.

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Polarization behavior of bone marrow-derived macrophages on charged P(VDF-TrFE) coatings

Abstract: The immune response of bone implants is closely related to the interaction between macrophages and biomaterial surfaces.

Cited by 26 publications

References 30 publications

Janus electro-microenvironment membrane with surface-selective osteogenesis/gingival healing ability for guided bone regeneration

Janus electro-microenvironment membrane with surface-selective osteogenesis/gingival healing ability for guided bone regeneration

Effects of electrical stimulation on cytokine‐induced macrophage polarization

Bone Repair Biomaterials: A Perspective from Immunomodulation

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