Polarization-sensitive reflectance imaging in skeletal muscle

Li, Xin; Ranasinghesagara, Janaka; Yao, Gang

doi:10.1364/oe.16.009927

Cited by 34 publications

(30 citation statements)

References 25 publications

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“…2), more V-polarized incident light is backscattered along the x axis; while more H-polarized incident light is backscattered along the y axis. This phenomenon can be qualitatively explained if considering the polarization-dependent sarcomere diffraction as discussed in our recent study [18]. The sarcomeres diffract the majority of V-polarized light to the directions perpendicular to muscle fibers.…”

Section: Resultsmentioning

confidence: 67%

“…The images are labeled using two letters: the first letter indicates the incident polarization and the second letter indicates the detection polarization. The 16 images reveal the unique rhombus-shaped reflectance patterns in skeletal muscles [17,18]. The VV image has the largest intensity and the HH image shows the most significant rhombuslike shape.…”

Section: Resultsmentioning

confidence: 99%

“…The full Mueller matrices were calculated from a total of 16 polarization reflectance images recorded in the sample using the same method described before [18]. In our measurements, three different linearly polarized states were used: H, V, and P, whose polarization directions were aligned with the x axis, the y axis, and at 45°to the x axis, respectively.…”

Section: A Experimental Methodsmentioning

confidence: 99%

“…Sarcomeres are the fundamental functional unit in each muscle fiber. Our recent studies have shown that sarcomeres play important roles in modulating both unpolarized [17] and polarized [18] light propagation in muscle. However, the interpretation of the measured Mueller matrix in muscle is not fully understood.…”

Section: Introductionmentioning

confidence: 99%

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Mueller matrix decomposition of diffuse reflectance imaging in skeletal muscle

Yao

2009

Appl. Opt.

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Propagation of polarized light in skeletal muscle is significantly affected by anisotropic muscle structures. To completely characterize muscle polarization properties, we acquired the whole Mueller matrix images of the diffuse reflectance. A polar decomposition algorithm was applied to extract the individual diattenuation, retardance, and depolarization images from the measured Mueller matrix. The decomposed polarization properties in muscle show distinctly different patterns from those obtained in isotropic scattering media. Stretching the prerigor muscle sample induced clear changes in the raw polarization reflectance images. However, muscle stretching induced minimal changes in the decomposed Mueller matrix images.

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Section: Resultsmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Section: A Experimental Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mueller matrix decomposition of diffuse reflectance imaging in skeletal muscle

Yao

2009

Appl. Opt.

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“…As in any fibrous tissues, skeletal muscles show strong optical birefringence because the light experiences different optical refractive indices when propagating along and perpendicular to the muscle fiber [8]. Muscle damage disrupts the myofiber organization and thus can change the muscle birefringent properties.…”

Section: Introductionmentioning

confidence: 99%

Optical polarization tractography revealed significant fiber disarray in skeletal muscles of a mouse model for Duchenne muscular dystrophy

Wang¹,

Zhang²,

Wasala³

et al. 2015

Biomed. Opt. Express

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Optical polarization tractography (OPT) was recently developed to visualize tissue fiber architecture with cellular-level resolution and accuracy. In this study, we explored the feasibility of using OPT to study muscle disease in the mdx4cv mouse model of Duchenne muscular dystrophy. The freshly dissected tibialis anterior muscles of mdx4cv and normal mice were imaged. A "fiber disarray index" (FDI) was developed to quantify the myofiber disorganization. In necrotic muscle regions of the mdx4cv mice, the FDI was significantly elevated and can be used to segment the 3D necrotic regions for assessing the overall muscle damage. These results demonstrated the OPT's capability for imaging microscopic fiber alternations in muscle research.

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Monitoring microstructural variations of fresh skeletal muscle tissues by Mueller matrix imaging

Chang

et al. 2016

Journal of Biophotonics

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Recently many attempts have been made for extracting the structural information of myofibrils as indicators for diseases of skeletal muscle. In this paper we adopt wide-field illumination and take the backscattering Mueller matrix images of bovine skeletal muscle tissues during the 24-hour experimental time after the animal's death. The 2D images of Mueller matrix elements and their frequency distribution histograms (FDHs) reveal rich qualitative information on the changes in the microstructures of the skeletal muscle. The temporal variations of the sample are quantitatively analyzed using two Mueller matrix transformation (MMT) parameters. The characteristic features of the temporal plots are attributed to the rigor mortis and proteolysis processes. For a deeper insight on the relationship between the features of the MMT parameters and the microstructures during the rigor mortis and proteolysis processes, Monte Carlo (MC) simulations are carried out based on sphere-cylinder birefringence model (SCBM). The good agreement between the experimental and MC simulated results show that the FDHs and MMT parameters can describe more clearly the characteristic microstructural features of skeletal muscle tissues. The techniques are useful for the characterization of physiological status of tissues, or quantitative assessment of meat qualities in food industry.

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Polarization-sensitive reflectance imaging in skeletal muscle

Cited by 34 publications

References 25 publications

Mueller matrix decomposition of diffuse reflectance imaging in skeletal muscle

Mueller matrix decomposition of diffuse reflectance imaging in skeletal muscle

Optical polarization tractography revealed significant fiber disarray in skeletal muscles of a mouse model for Duchenne muscular dystrophy

Monitoring microstructural variations of fresh skeletal muscle tissues by Mueller matrix imaging

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