Pollen grains infected with apple stem grooving virus serve as a vector for horizontal transmission of the virus

Isogai, Masamichi; Shimoda, Ren; Nishimura, Honoka; Yaegashi, Hajime

doi:10.1007/s10327-021-01039-0

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…Pollinator-mediated virus transmission is an emerging field of study in which an ample amount of research is needed to understand the underlying mechanisms of pollen-borne viruses so that sustainable management strategies for viral diseases can be developed (Darzi et al 2018, Adler et al 2021, Proesmans et al 2021, Isogai et al 2022). Although other modes of direct virus transmission by arthropods such as circulative propagative (e.g., thrips and tomato spotted wilt virus), circulative non-propagative (e.g.…”

Section: Discussionmentioning

confidence: 99%

Bees and thrips carry virus-positive pollen in peach orchards in South Carolina, United States

Tayal

Wilson

Cieniewicz

2023

Journal of Economic Entomology

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Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) are pollen-borne viruses of important stone fruit crops, including peaches, which can cause substantial yield loss. Although both horizontal and vertical (i.e., seed) transmission of both viruses occurs through pollen, the role of flower-visiting insects in their transmission is not well understood. Bees and thrips reportedly spread PNRSV and PDV in orchards and greenhouse studies; however, the field spread of PNRSV and PDV in peach orchards in the southeastern United States is not explored. We hypothesized that bees and thrips may facilitate virus spread by carrying virus-positive pollen. Our 2-yr survey results show that 75% of captured bees are carrying virus-positive pollen and moving across the orchard while a subsample of thrips were also found virus positive. Based on morphology, Bombus, Apis, Andrena, Eucera, and Habropoda are the predominant bee genera that were captured in peach orchards. Understanding the role of bees and thrips in the spread of PNRSV and PDV will enhance our understanding of pollen-borne virus ecology.

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Section: Discussionmentioning

confidence: 99%

Bees and thrips carry virus-positive pollen in peach orchards in South Carolina, United States

Tayal

Wilson

Cieniewicz

2023

Journal of Economic Entomology

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“…ASGV is generally seed and mechanically transmitted, and no natural vectors have been reported ( Kim et al, 2021 ). However, a recent study revealed that Nicotiana benthamina plants were infected with ASGV through pollination with ASGV-infected pollen grains derived from Malus pumila , indicating that pollen grains possibly served as a vector for horizontal pollen transmission of ASGV ( Isogai et al, 2022 ). To date, 13 plant viruses, including CLBV and CMV, have been proven to naturally infect kiwifruit plants ( Blouin et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Metatranscriptomic Analysis of Plant Viruses in Imported Pear and Kiwifruit Pollen

Lee

Jeong

2022

Plant Pathol J

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Pollen is a vector for viral transmission. Pollen-mediated viruses cause serious economic losses in the fruit industry. Despite the commercial importance of pollen-associated viruses, the diversity of such viruses is yet to be fully explored. In this study, we performed metatranscriptomic analyses using RNA sequencing to investigate the viral diversity in imported apple and kiwifruit pollen. We identified 665 virus-associated contigs, which corresponded to four different virus species. We identified one virus, the apple stem grooving virus, from pear pollen and three viruses, including citrus leaf blotch virus, cucumber mosaic virus, and lychnis mottle virus in kiwifruit pollen. The assembled viral genome sequences were analyzed to determine phylogenetic relationships. These findings will expand our knowledge of the virosphere in fruit pollen and lead to appropriate management of international pollen trade. However, the pathogenic mechanisms of pollen-associated viruses in fruit trees should be further investigated.

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“…transmitted by seed and pollen [27,45,54,55]. Some ilarviruses can be transmitted by thrips and other flower-visiting arthropods.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Rose Virome Analysis and Identification of A Novel Ilarvirus In Taiwan

Chen¹,

Lin²,

Lin³

et al. 2022

Preprint

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Rose (Rosa spp.), especially R. hybrida, is one of the most popular ornamental plants in the world and the third largest cut flower crop in Taiwan. Rose mosaic disease (RMD), showing mosaic, line patterns and ringspots on leaves, is a common rose disease caused by complex infection of various viruses. Due to pests and diseases, the rose planting area in Taiwan has been decreasing since 2008; however, no rose virus disease has been reported in the past five decades. In the spring of 2020, rose samples showing RMD-like symptoms were observed at an organic farm in Chiayi, central Taiwan. The virome in the farm was analyzed by RNA-seq. Rose genomic sequences were filtered from the obtained reads. The remaining reads were de novo assembled to generate 294 contigs, 50 of which were annotated as viral sequences corresponding to 10 viruses. Through reverse transcription-polymerase chain reaction validation, a total of seven viruses were detected, including six known rose viruses, namely apple mosaic virus, prunus necrotic ringspot virus, rose partitivirus, apple stem grooving virus, rose spring dwarf-associated virus and rose cryptic virus 1, and a novel ilarvirus. After completing the whole genome sequencing and sequence analysis, the unknown ilarvirus was demonstrated as a new species, tentatively named rose ilarvirus 2. This is the first report of the rose viruses in Taiwan.

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Pollen grains infected with apple stem grooving virus serve as a vector for horizontal transmission of the virus

Cited by 7 publications

References 21 publications

Bees and thrips carry virus-positive pollen in peach orchards in South Carolina, United States

Bees and thrips carry virus-positive pollen in peach orchards in South Carolina, United States

Metatranscriptomic Analysis of Plant Viruses in Imported Pear and Kiwifruit Pollen

Rose Virome Analysis and Identification of A Novel Ilarvirus In Taiwan

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