Pollen Viability an Index of Abiotic Stresses Tolerance and Methods for the Improved Pollen Viability

Razzaq, Muhammad Khuram; Rauf, Saeed; Khurshid, Mohsin; Iqbal, Shahid; Bhat, Javid Ahmad; Farzand, Ayaz; Riaz, Adeel; GuangNan, Xing; Gai, Junyi

doi:10.17582/journal.pjar/2019/32.4.609.624

Cited by 22 publications

(17 citation statements)

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“…Some genes have been engineered to develop stress (biotic and abiotic) resistance in crop plants [ 18 , 19 , 20 , 21 , 22 ]. Numerous new candidate genes have been discovered from wild crop relative genomics for stress (abiotic and biotic) tolerance in crops [ 2 , 23 ]. For example, a high-density buckwheat complete genome sequencing genomic map, Hi-C online accessible sequencing data, and fosmid DNA libraries [ 17 ].…”

Section: Multi-omics Technologymentioning

confidence: 99%

“…The development of high-yielding varieties exposed to stress depends on direct selection for yield stability in multiple locations. Germplasm development with tolerance to biotic and abiotic factors is important for sustainable crop production [ 1 , 2 ]. The molecular term “omics” suggests a comprehensive assessment of numerous molecules [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Omics and CRISPR-Cas9 Approaches for Molecular Insight, Functional Gene Analysis, and Stress Tolerance Development in Crops

Razzaq

Aleem

Mansoor

et al. 2021

IJMS

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Plants are regularly exposed to biotic and abiotic stresses that adversely affect agricultural production. Omics has gained momentum in the last two decades, fueled by statistical methodologies, computational capabilities, mass spectrometry, nucleic-acid sequencing, and peptide-sequencing platforms. Functional genomics—especially metabolomics, transcriptomics, and proteomics—have contributed substantially to plant molecular responses to stress. Recent progress in reverse and forward genetics approaches have mediated high-throughput techniques for identifying stress-related genes. Furthermore, web-based genetic databases have mediated bioinformatics techniques for detecting families of stress-tolerant genes. Gene ontology (GO) databases provide information on the gene product’s functional features and help with the computational estimation of gene function. Functional omics data from multiple platforms are useful for positional cloning. Stress-tolerant plants have been engineered using stress response genes, regulatory networks, and pathways. The genome-editing tool, CRISPR-Cas9, reveals the functional features of several parts of the plant genome. Current developments in CRISPR, such as de novo meristem induction genome-engineering in dicots and temperature-tolerant LbCas12a/CRISPR, enable greater DNA insertion precision. This review discusses functional omics for molecular insight and CRISPR-Cas9-based validation of gene function in crop plants. Omics and CRISPR-Cas9 are expected to garner knowledge on molecular systems and gene function and stress-tolerant crop production.

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Section: Multi-omics Technologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Omics and CRISPR-Cas9 Approaches for Molecular Insight, Functional Gene Analysis, and Stress Tolerance Development in Crops

Razzaq

Aleem

Mansoor

et al. 2021

IJMS

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“…Two genotypes, EC-620376 and Pusa Ruby, exhibited non significant changes betwixt the season. Continuous exposure of tomato plants to high temperature impart differential accumulation of hexose Vs sucrose in developing pollen grains (Paupiere et al 2014) and delayed tapetum development (Alsamir et al 2017;Razzaq et al 2019), thereupon imparting different levels of tolerance. Metabolic alterations in the thermo-tolerant genotypes leads to the proline accumulation, acting as a heat shock protein (Giorno et al 2003;Sato et al 2006;Din et al 2015;Saeed et al 2017;Xu et al 2017b).…”

Section: Pollen Viability (%)mentioning

confidence: 99%

Application of stress susceptibility index and multivariate analysis of tomato (Solanum lycopersicum L.) to identify thermo-tolerant genotypes

Archana¹,

Pradeepkumar²,

Gopinath³

et al. 2021

Preprint

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Climatic parameters have become key mechanisms in controlling crop productivity worldwide. The more frequency of heat waves urges the breeding for thermo-tolerance. The motive of this study was to furnish an accurate and deep understanding on the heat tolerance of reproductive key traits (flowers with exerted stigma, pollen viability, fruits set per cent and number of fruits per cluster), along with earliness (days to first fruit set) and yield traits (average fruit weight, pericarp thickness, number of fruits per plant and yield per plant) in tomato. The study reasoned 35 genotypes employing three no-identical analysis tools, ANOVA of field assessment, stress susceptibility index and multivariate analysis based on genetic diversity. Insights of genetic architecture of the reproductive traits under heat stress might improve our core understanding, and might have applied value. The study substantiated the essence of heat tolerance for the genotypes EC-620395, EC-620401, EC-620406 and EC-620410. Divergence analysis revealed five clusters corresponding to specific characters. Therefore on the ground of the investigation new breeding lines and breeding strategies can be implemented under changing environmental conditions with special reference to elevated temperature.

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“…Exposure of 35 °C light and 25 °C dark stress for 48 h specifically at pollination stage negatively affects gas exchange and chlorophyll content in addition to pollen fertility ( Sunoj et al, 2017 ). Heat stress and low relative moisture humidity reduce pollen viability; thus they can be used to distinguish crop genotypes against various infield stresses ( Razzaq et al, 2019 ). The seed yield is affected by the extreme temperatures.…”

Section: Introductionmentioning

confidence: 99%

Morpho-physiological effects of environmental stress on yield and quality of sweet corn varieties (Zea mays L.)

Taş

Mutlu

2021

PeerJ

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Sweet corn is cultivated in different climatic regions of the world, and consumed either fresh or processed. Morpho-physiological effects of environmental stress on yield, yield components and quality of some sweet corn varieties were investigated in field experiments conducted at Southeastern Anatolia Region of Turkey during 2019 and 2020 growing seasons. The experimental lay out was randomized blocks with three replicates. Eight candidates and two control sweet corn varieties classified as moderate maturity (FAO 650–700) were used in field experiment. Mean values of pollen fertility rate (PFR, %), total soluble solids (TSS, °Brix), abscisic acid (ABA, nmol/g DW), ear length (EL, cm), plant height (PH, cm), number of grains per cob (CGN, grain) and fresh cob yield (FCY, t ha−1) were significantly different between years and sweet corn varieties. The PFR, TSS, ABA, EL, PH, CGN and FCY ranged from 40.29–67.65%, 13.24–20.09 °brix, 7.74–21.04 nmol/g DW, 9.69–15.98 cm, 97.80–171.34 cm, 289.15–420.33 grain and 4.15–10.23 t ha−1 respectively. The FCY, yield components and PFR values in the second year that had a higher temperature and lower relative humidity were lower compared to the first year, while ABA and TSS values were higher in the second year. Statistically significant correlations were recorded between the parameters investigated except FCY and TSS. The FCY and other parameters of sweet corn varieties, which produced high ABA phytohormone, were high, and the ABA hormone significantly contributed to plant growth under stress conditions. The results revealed that the PFR physiological parameter and ABA hormone in the plants provide important information about stress level and stress tolerance level of the cultivars, respectively. Despite adverse environmental stress conditions, the FCY of ŞADA-18.7 variety, one of the candidate varieties, was higher than that of the control and the mean value of the experiment.

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Pollen Viability an Index of Abiotic Stresses Tolerance and Methods for the Improved Pollen Viability

Cited by 22 publications

References 0 publications

Omics and CRISPR-Cas9 Approaches for Molecular Insight, Functional Gene Analysis, and Stress Tolerance Development in Crops

Omics and CRISPR-Cas9 Approaches for Molecular Insight, Functional Gene Analysis, and Stress Tolerance Development in Crops

Application of stress susceptibility index and multivariate analysis of tomato (Solanum lycopersicum L.) to identify thermo-tolerant genotypes

Morpho-physiological effects of environmental stress on yield and quality of sweet corn varieties (Zea mays L.)

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