Poly-3-hydroxybutyrate (PHB) production by Bacillus flexus ME-77 using some industrial wastes

El‐Sheekh, Mostafa M.; El-Abd, Mostafa A; El‐Diwany, Ahmed I.; Ismail, Abdel‐Mohsen S.; Omar, Thanaa H.

doi:10.1007/s12210-014-0368-z

Cited by 14 publications

(5 citation statements)

References 37 publications

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“…produced 56% (5.0 g/L) of PHB using sugarcane bagasse hydrolysates [ 85 ]. Bacillus flexus ME-77 produced 15% (4.5 g/L) of PHB using sugarcane molasses [ 86 ]. B. thuringiensis SBC4 produced 43.95% (0.4 g/L) of PHB using glucose and corn cob [ 87 ].…”

Section: Resultsmentioning

confidence: 99%

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

et al. 2021

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Cheese whey (CW) can be an excellent carbon source for polyhydroxyalkanoates (PHA)-producing bacteria. Most studies have used CW, which contains high amounts of lactose, however, there are no reports using raw CW, which has a relatively low amount of lactose. Therefore, in the present study, PHA production was evaluated in a two-stage process using the CW that contains low amounts of lactose. In first stage, the carbon source existing in CW was converted into acetic acid using the bacteria, Acetobacter pasteurianus C1, which was isolated from food waste. In the second stage, acetic acid produced in the first stage was converted into PHA using the bacteria, Bacillus sp. CYR-1. Under the condition of without the pretreatment of CW, acetic acid produced from CW was diluted at different folds and used for the production of PHA. Strain CYR-1 incubated with 10-fold diluted CW containing 5.7 g/L of acetic acid showed the higher PHA production (240.6 mg/L), whereas strain CYR-1 incubated with four-fold diluted CW containing 12.3 g/L of acetic acid showed 126 mg/L of PHA. After removing the excess protein present in CW, PHA production was further enhanced by 3.26 times (411 mg/L) at a four-fold dilution containing 11.3 g/L of acetic acid. Based on Fourier transform infrared spectroscopy (FT-IR), and 1H and 13C nuclear magnetic resonance (NMR) analyses, it was confirmed that the PHA produced from the two-stage process is poly-β-hydroxybutyrate (PHB). All bands appearing in the FT-IR spectrum and the chemical shifts of NMR nearly matched with those of standard PHB. Based on these studies, we concluded that a two-stage process using Acetobacter pasteurianus C1 and Bacillus sp. CYR-1 would be applicable for the production of PHB using CW containing a low amount of lactose.

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Section: Resultsmentioning

confidence: 99%

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

et al. 2021

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“…Similarly, [34] found that using corn steep liquor as source of nitrogen among others increased both growth and PHA production by Bacillus Flexus ME-77. Jangra and Nehra (2017) also informed that Bacillus and Pseudomonas species showed different preference on mustard cake and other nitrogen substrate utilized for PHA production [33].…”

Section: Discussionmentioning

confidence: 84%

“…PHA production by gram-positive bacteria such as Bacillus sp has been reported to have several advantages over others due to absence of lipopolysaccharides layer, high protein secretion and capacity to utilize cheap raw materials [33]. Amidst 50 bacteria strains examined quantitatively by [34], Bacillus flexus Azu-A2 gave maximum PHA yield of 0.880 g/l and was chosen as most efficient while work of [35] obtained a yield of 0.186 g/l by a promising producer of PHA identified as Bacillus flexus isolated from rhizopheric soil sample.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization, Optimization and Production of PHA by Indigenous Bacteria Using Alternative Nutrient Sources as Substrate

Dienye

Agwa

Abu

2022

MRJI

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Polyhydroxyalkanoates (PHA) are renewable, biodegradable biopolymer intracellularly accumulated by wide array of microorganisms as carbon reserve. This study investigates the influence of various cultural conditions on PHA production by a recently isolated local species under submerged fermentation. Six PHA producing strains were identified by 16S rDNA gene sequencing and strain Priestia flexa OWO1 showed satisfactory PHA productivity. The effects of production parameters were investigated and extraction of PHA was carried out using sodium hypochlorite method and maximum amount was detected after 72h. Maximum PHA production was obtained at incubation period of 48h, pH of 7.0 and temperature of 30oC. Amongst the hydrolysate of agro waste used, brewers spent grain (BSG) gave maximum yield of 3.01g/L while beans bran powder gave the highest PHA yields of 3.9 g/L amongst the organic nitrogen sources tested. Analysis of the crude PHA by Fourier Transform Infrared Spectroscopy (FT-IR) showed the presence of methyl, methylene as well as carbonyl functional groups. PHA production was higher after optimizing the production conditions as compared to basal medium therefore the utilization of these cheap renewable resources as alternative substrates for production of PHA make the process cost effective and sustainable.

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“…Uma cepa de Haloferax mediterranei chegou a acumular 70% de seu peso seco em PHAs tendo como substrato a vinhaça, resíduo da indústria de álcool de difícil disposição devido ao pH ácido e características poluentes (BHATTACHARYYA, et al, 2012). As cepas Bacillus flexus ME-77, Pseudomonas corrugata 388 e Bacillus subtilis OK2 obtiveram 51, 42 e 40% de PHA/célula quando melaço de cana de açúcar, resíduos da produção de biodiesel à base de soja e casca de laranja, respectivamente, foram empregados como substratos (ASHBY, et al, 2004;SUKAN, et al, 2014;EL-SHEEKH, et al, 2015).…”

Section: Fundamentação Teóricaunclassified

“…De acordo com Doudoroff e Stanier (1959) e Valappil et al (2007) este fato pode estar relacionado a liberação de compostos tóxicos liberados pela bactéria durante a produção de P(3HB).…”

Section: Triagem De Bactérias Usando Hidrolisado Da Torta De Tabacounclassified

Aproveitamento de torta do tabaco gerado na produção de biodiesel para a obtenção de bioplástico

Riça

Moraes

Souza

et al. 2016

RJP

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O poli 3-hidroxibutirato – P(3HB) – é o principal polímero da classe dos poli hidroxialcanoatos (PHAs), polímeros biodegradáveis sintetizados por microrganismos a partir de diversos substratos. O objetivo desse trabalho foi a produção de P(3HB) a partir do resíduo da extração do óleo da semente de tabaco (Nicotiana tabacum L.) como substrato alternativo e de baixo custo para triagem de microrganismos produtores de P(3HB). O resíduo foi avaliado quanto a sua composição e beneficiado através de hidrólise. O hidrolisado foi utilizado em ensaio de fermentação com cepas bacterianas isoladas da Estação de Tratamento de Efluentes (ETE) da Universidade de Santa Cruz do Sul (UNISC). Para visualização de inclusões lipofílicas nas células, indicadoras de acúmulo de P(3HB), utilizou-se coloração de Sudan Black. A extração do polímero do interior das células foi realizada utilizando-se clorofórmio e hipoclorito de sódio e o produto extraído foi analisado em espectrofotômetro. De 25 isolados, a cepa E10 foi a que apresentou melhor resultado frente à coloração de Sudan Black. A análise do produto obtido sugere que a E10 produziu PHAs utilizando o resíduo de tabaco, demonstrando seu potencial como substrato de baixo custo para crescimento microbiano e obtenção de biopolímeros.

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Poly-3-hydroxybutyrate (PHB) production by Bacillus flexus ME-77 using some industrial wastes

Cited by 14 publications

References 37 publications

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

Molecular Characterization, Optimization and Production of PHA by Indigenous Bacteria Using Alternative Nutrient Sources as Substrate

Aproveitamento de torta do tabaco gerado na produção de biodiesel para a obtenção de bioplástico

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