Poly(A) mRNA is attached to insect ovarian microtubules in vivo in a nucleotide-sensitive manner

Stephen, Susan; Talbot, Nicholas J.; Stebbings, Howard

doi:10.1002/(sici)1097-0169(1999)43:2<159::aid-cm7>3.0.co;2-u

Cited by 11 publications

(8 citation statements)

References 44 publications

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“…The nurse cell syncytium connects to all oocytes via elongated, microtubule-rich tubes called “nutritive tubes” (Hyams and Stebbings, 1979; Harrison et al, 1991) through which maternal factors, including mRNA, proteins and mitochondria, are transported to developing oocytes (Fig. 1A2,A3) (Stebbings et al, 1985; Stebbings and Hunt, 1987; Anastas et al, 1991; Hurst et al, 1999; Stephen et al, 1999). …”

Section: Resultsmentioning

confidence: 99%

Evidence against a germ plasm in the milkweed bugOncopeltus fasciatus, a hemimetabolous insect

2013

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SummaryPrimordial germ cell (PGC) formation in holometabolous insects like Drosophila melanogaster relies on maternally synthesised germ cell determinants that are asymmetrically localised to the oocyte posterior cortex. Embryonic nuclei that inherit this “germ plasm” acquire PGC fate. In contrast, historical studies of basally branching insects (Hemimetabola) suggest that a maternal requirement for germ line genes in PGC specification may be a derived character confined principally to Holometabola. However, there have been remarkably few investigations of germ line gene expression and function in hemimetabolous insects. Here we characterise PGC formation in the milkweed bug Oncopeltus fasciatus, a member of the sister group to Holometabola, thus providing an important evolutionary comparison to members of this clade. We examine the transcript distribution of orthologues of 19 Drosophila germ cell and/or germ plasm marker genes, and show that none of them localise asymmetrically within Oncopeltus oocytes or early embryos. Using multiple molecular and cytological criteria, we provide evidence that PGCs form after cellularisation at the site of gastrulation. Functional studies of vasa and tudor reveal that these genes are not required for germ cell formation, but that vasa is required in adult males for spermatogenesis. Taken together, our results provide evidence that Oncopeltus germ cells may form in the absence of germ plasm, consistent with the hypothesis that germ plasm is a derived strategy of germ cell specification in insects.

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Section: Resultsmentioning

confidence: 99%

Evidence against a germ plasm in the milkweed bugOncopeltus fasciatus, a hemimetabolous insect

2013

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“…The main molecular motors acting in insect oogenesis are myosin, dynein and kynesin, which are motor proteins associated with actin and microtubules (Stebbings et al, 1995;Jordan and Karess, 1997;Deng and Lin, 1997;Stephen et al, 1999;Geisbrecht and Montell, 2002;Pflanz et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…These movements are conducted by cytoskeletal elements that are present in the germ cells in the form of actin and microtubules and their motor proteins (Gutzeit et al, 1991;Bohrmann and Biber, 1994;Bü ning, 1994;Deng and Lin 1997;Bilinsky and Jarglaz, 1999). Therefore, in ovary germaria, the incomplete cytokinesis that originates the cytoplasmic bridges among cystocytes depends on actin filaments that act simultaneously, maintaining the ring canals open and regulating their wideness (Robinson et al, 1994;Tilney, et al, 1996;Patrício and Cruz-Landim, 2006), while the microtubules transport cell components through the canals (Stebbings and Hunt, 1987;Stebbings et al, 1995;Stephen et al, 1999;Schnorrer et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Characterization and localization of dynein and myosins V and VI in the ovaries of queen bees

Patrício

Calábria²,

Peixoto

et al. 2010

Cell Biology International

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The presence of myosin and dynein in the ovaries of both Apis mellifera and Scaptotrigona postica was investigated in extracts and in histological sections. In the ovary extracts, motor proteins, myosins V, VI and dynein were detected by Western blot. In histological sections, they were detected by immunocytochemistry, using a mouse monoclonal antibody against the intermediary chain of dynein and a rabbit polyclonal antibody against the myosin V head domain. The myosin VI tail domain was recognized by a pig polyclonal antibody. The results show that these molecular motors are expressed in the ovaries of both bee species with few differences in location and intensity, in regions where movement of substances is expected during oogenesis. The fact that antibodies against vertebrate proteins recognize proteins of bee species indicates that the specific epitopes are evolutionarily well preserved.

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“…Using hybridization techniques (Stephen et al, 1999) and antibodies (Hurst et al, 1999), mRNA and a Stau-like protein have been shown to be attached to the microtubules under nucleotide conditions which increase the affinity of motor proteins for microtubules. Using hybridization techniques (Stephen et al, 1999) and antibodies (Hurst et al, 1999), mRNA and a Stau-like protein have been shown to be attached to the microtubules under nucleotide conditions which increase the affinity of motor proteins for microtubules.…”

mentioning

confidence: 99%

“…Additional evidence for the interaction between Stau and microtubules being motor-dependent has come from studies of a different insect, Notonecta the backswimmer, which has comparable oogenesis to Drosophila, but from which large bundles of native microtubules connecting the nurse cells to the oocytes can be dissected manually. Using hybridization techniques (Stephen et al, 1999) and antibodies (Hurst et al, 1999), mRNA and a Stau-like protein have been shown to be attached to the microtubules under nucleotide conditions which increase the affinity of motor proteins for microtubules. Biochemical analysis of these native microtubule complexes showed them to comprise tubulins, microtubule-associated proteins and motor proteins, as expected, but also additional species including the Stau-like protein.…”

mentioning

confidence: 99%

Unravelling mRNA transport complexes from insect ovaries

Stebbings

2000

Biology of the Cell

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Poly(A) mRNA is attached to insect ovarian microtubules in vivo in a nucleotide-sensitive manner

Cited by 11 publications

References 44 publications

Evidence against a germ plasm in the milkweed bugOncopeltus fasciatus, a hemimetabolous insect

Evidence against a germ plasm in the milkweed bugOncopeltus fasciatus, a hemimetabolous insect

Characterization and localization of dynein and myosins V and VI in the ovaries of queen bees

Unravelling mRNA transport complexes from insect ovaries

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