Poly(d,l-lactic acid) surfaces modified by silk fibroin: effects on the culture of osteoblast in vitro

Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells

“…Therefore, the degraded silk fibroin fragments appear to result in an increase in the expression of ALP. Cai et al (13) have reported that a poly (D,L-lactic acid) surface modified with 30 kDa of silk fibroin showed more ALP activity than an untreated poly (D,L-lactic acid) surface. In addition, MC3T3-E1 cells cultured on a silk fibroin membrane were reported to show ALP activity levels comparable to those on a surfacetreated culture dish (14).…”

Mesopore Bioglass/Silk Composite Scaffolds for Bone Tissue Engineering

“…When applied to the MBG scaffolds this silk may provide a relatively stable surface interface to support BMSC proliferation and differentiation. It has been reported that silk-functionalized titanium surfaces can enhance osteoblast functions [30,31], and also that silk modification of poly (D,L-lactic acid) improves ostoblast differentiation [32]. Although the mechanisms underlying this stimulatory effect on cell functions remains unclear, the explanation may be related to the interaction with specific chemical groups in silk, such as amino acids [15].…”

Preparation and Characterization of Chitosan-Coated Poly(l-Lactic Acid) Fibers and Their Braided Rope

“…However, PLA also suffers some shortcomings such as low hydrolyzability and poor adhesion and growth of cells because the PLA surface is hydrophobic. Recently, some PLA surface modification techniques have been reported, including alkali treatment, enzymatic treatment, plasma techniques, coating with a hydrophilic polymer, and crosslinking with proteins, alginate, and chitosan (CS) [12][13][14][15][16][17][18][19]. In particular, alkali treatment resulted in remarkable decreases of the contact angles on the PLA film and increases in bone cell functions and chondrocyte attachment and growth [20][21][22].…”